Cloned (Comment) | Organism |
---|---|
gene AcC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli | Aquilaria crassna |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme has no N-terminal transit peptide | Aquilaria crassna | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Aquilaria crassna | - |
alpha-guaiene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Aquilaria crassna | - |
delta-guaiene + diphosphate | - |
? | |
additional information | Aquilaria crassna | the enzyme catalyzes the production of three sesquiterpenes, alpha-guaiene, alpha-humulene, and delta-guaiene | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquilaria crassna | D0VMR6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme has no N-terminal transit peptide | Aquilaria crassna |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Aquilaria crassna | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | alpha-guaiene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | delta-guaiene + diphosphate | - |
? | |
additional information | the enzyme catalyzes the production of three sesquiterpenes, alpha-guaiene, alpha-humulene, and delta-guaiene | Aquilaria crassna | ? | - |
? | |
additional information | solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS) analysis of the enzyme reaction products | Aquilaria crassna | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACC2 | - |
Aquilaria crassna |
delta-guaiene synthase 1 | - |
Aquilaria crassna |
Organism | Comment | Expression |
---|---|---|
Aquilaria crassna | methyl jasmonate induces the expression of enzyme delta-guaiene synthase, at 6 h after methyl jasmonate treatment, the expression of the delta-guaiene synthase gene has increased approximately 150fold resulting in 240 pmol/mg protein/h delta-guaiene production | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthetic pathways for sesquiterpenes found in agarwood and cell suspension cultures, putative pathway, overview. Various post-transcriptional, translational, or post-translational regulatory mechanisms affecting the expression or activity of alpha-humulene and delta-guaiene synthases are possible | Aquilaria crassna |
additional information | the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site | Aquilaria crassna |
physiological function | the enzyme is involved in the biosynthesis of the three sesquiterpenes, alpha-guaiene (cf. EC 4.2.3.87), alpha-humulene (cf. EC 4.2.3.104), and delta-guaiene, only very small amounts of alpha-humulene are produced | Aquilaria crassna |