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Literature summary for 4.2.3.7 extracted from

  • Rosso, C.; Ermondi, G.; Caron, G.
    GRID/BIOCUBE4mf to rank the influence of mutations on biological processes to design ad hoc mutants (2015), Med. Chem. Res., 24, 2612-2620 .
No PubMed abstract available
Search for more literature for 4.2.3.7

Protein Variants

Protein Variants Comment Organism
D80E the mutation influences binding of the required divalent Mg2+ cofactor Streptomyces exfoliatus
D81E the mutation influences binding of the required divalent Mg2+ cofactor Streptomyces exfoliatus
D84E the mutation influences binding of the required divalent Mg2+ cofactor Streptomyces exfoliatus
F77Y the mutant exhibits nearly full wild type activity Streptomyces exfoliatus
H309A the mutant exhibits nearly full wild type activity Streptomyces exfoliatus
H309C the mutant exhibits nearly full wild type activity Streptomyces exfoliatus
H309F the mutant exhibits nearly full wild type activity Streptomyces exfoliatus
H309S the mutant exhibits nearly full wild type activity Streptomyces exfoliatus
N219D the mutation influences binding of the required divalent Mg2+ cofactor Streptomyces exfoliatus
N219L the mutation influences binding of the required divalent Mg2+ cofactor Streptomyces exfoliatus
W308F the mutant exhibits nearly full wild type activity Streptomyces exfoliatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00016
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W308F, pH and temperature not specified in the publication Streptomyces exfoliatus
0.00019
-
 (2E,6E)-farnesyl diphosphate mutant enzyme H309A, pH and temperature not specified in the publication Streptomyces exfoliatus
0.00025
-
 (2E,6E)-farnesyl diphosphate mutant enzyme H309S, pH and temperature not specified in the publication Streptomyces exfoliatus
0.0003
-
 (2E,6E)-farnesyl diphosphate mutant enzyme F77Y, pH and temperature not specified in the publication Streptomyces exfoliatus
0.00033
-
 (2E,6E)-farnesyl diphosphate mutant enzyme H309C, pH and temperature not specified in the publication Streptomyces exfoliatus
0.00143
-
 (2E,6E)-farnesyl diphosphate mutant enzyme H309F, pH and temperature not specified in the publication Streptomyces exfoliatus
0.00228
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D84E, pH and temperature not specified in the publication Streptomyces exfoliatus
0.0026
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D81E, pH and temperature not specified in the publication Streptomyces exfoliatus
0.00698
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D80E, pH and temperature not specified in the publication Streptomyces exfoliatus
0.0166
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N219D, pH and temperature not specified in the publication Streptomyces exfoliatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Streptomyces exfoliatus
-
 pentalenene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Streptomyces exfoliatus UC5319
-
 pentalenene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces exfoliatus Q55012
-
-
Streptomyces exfoliatus UC5319 Q55012
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Streptomyces exfoliatus pentalenene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Streptomyces exfoliatus UC5319 pentalenene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
PenA
-
 Streptomyces exfoliatus