Literature summary for 4.2.3.154 extracted from

Asamizu, S.; Xie, P.; Brumsted, C.J.; Flatt, P.M.; Mahmud, T.
Evolutionary divergence of sedoheptulose 7-phosphate cyclases leads to several distinct cyclic products (2012), J. Am. Chem. Soc., 134, 12219-12229.

Search for more literature for 4.2.3.154

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Nostoc punctiforme
expression in Escherichia coli	Trichormus variabilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0216	-	D-sedoheptulose 7-phosphate	pH 7.4, 25°C	Trichormus variabilis
0.0651	-	D-sedoheptulose 7-phosphate	pH 7.4, 25°C	Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-sedoheptulose 7-phosphate	Nostoc punctiforme	the enzyme is involved in the biosynthesis of the sunscreen compound shinorine	demethyl-4-deoxygadusol + phosphate + H2O	-	?
D-sedoheptulose 7-phosphate	Trichormus variabilis	the enzyme is involved in the biosynthesis of the sunscreen compound shinorine	demethyl-4-deoxygadusol + phosphate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Nostoc punctiforme	-	-	-
Trichormus variabilis	Q3M6C3	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Nostoc punctiforme
-	Trichormus variabilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-sedoheptulose 7-phosphate	-	Nostoc punctiforme	demethyl-4-deoxygadusol + phosphate + H2O	demethyl-4-deoxygadusol i.e. 2,3,5-trihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one	?
D-sedoheptulose 7-phosphate	-	Trichormus variabilis	demethyl-4-deoxygadusol + phosphate + H2O	demethyl-4-deoxygadusol i.e. 2,3,5-trihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one	?
D-sedoheptulose 7-phosphate	the enzyme is involved in the biosynthesis of the sunscreen compound shinorine	Nostoc punctiforme	demethyl-4-deoxygadusol + phosphate + H2O	-	?
D-sedoheptulose 7-phosphate	the enzyme is involved in the biosynthesis of the sunscreen compound shinorine	Trichormus variabilis	demethyl-4-deoxygadusol + phosphate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
Ava_3858	-	Trichormus variabilis
DDGS	-	Nostoc punctiforme
DDGS	-	Trichormus variabilis
desmethyl-4-deoxygadusol synthase	-	Nostoc punctiforme
desmethyl-4-deoxygadusol synthase	-	Trichormus variabilis
Npun_5600	-	Nostoc punctiforme

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Nostoc punctiforme
25	-	assay at	Trichormus variabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0533	-	D-sedoheptulose 7-phosphate	pH 7.4, 25°C	Trichormus variabilis
0.0833	-	D-sedoheptulose 7-phosphate	pH 7.4, 25°C	Nostoc punctiforme

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Nostoc punctiforme
7.4	-	assay at	Trichormus variabilis

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthesis of the sunscreen compound shinorine	Nostoc punctiforme
metabolism	the enzyme is involved in the biosynthesis of the sunscreen compound shinorine	Trichormus variabilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.2	-	D-sedoheptulose 7-phosphate	pH 7.4, 25°C	Nostoc punctiforme
2.47	-	D-sedoheptulose 7-phosphate	pH 7.4, 25°C	Trichormus variabilis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)