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Literature summary for 4.2.3.124 extracted from
- Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans (2005), Biotechnol. Lett., 27, 465-470.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene kanA, expression in Escherichia coli strains XL1-Blue MRF and BL21 (DE3), in the latter as His6-tagged protein, using plasmid pIBR25, formation of insoluble KanA, expression in Streptomyces lividans strain TK24 using pIBR25 with a MCS is successful | Streptomyces lividans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | required | Streptomyces lividans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Streptomyces lividans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | Streptomyces lividans | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces lividans | - |
gene kanA, encoded in the kanamycin gene cluster, no activity in strain TK24 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Streptomyces lividans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | - |
Streptomyces lividans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Streptomyces lividans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DOI synthase | - |
Streptomyces lividans |
| KanA | - |
Streptomyces lividans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Streptomyces lividans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
crude enzyme, 5 min, inactivation | Streptomyces lividans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Streptomyces lividans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | required | Streptomyces lividans | |
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