Application | Comment | Organism |
---|---|---|
synthesis | recombinant expression in Aspergillus nidulans resulting in production of gamma-terpinene. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds, e.g. gamma-terpinene | Citrus unshiu |
Cloned (Comment) | Organism |
---|---|
gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds | Citrus unshiu |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Citrus unshiu |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Citrus unshiu | - |
gamma-terpinene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus unshiu | Q6F5H0 | i.e. Citrus nobilis var. unshiu | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Citrus unshiu | gamma-terpinene + diphosphate | - |
? | |
additional information | product identification and quantification by GC-MS analysis | Citrus unshiu | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CitMTSL61 | - |
Citrus unshiu |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Citrus unshiu |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Citrus unshiu |