Any feedback?
No. of entries
Information on EC 4.2.3.114 - gamma-terpinene synthase
for references in articles please use BRENDA:EC4.2.3.114
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 4 Lyases
4.2 Carbon-oxygen lyases
4.2.3 Acting on phosphates
4.2.3.114 gamma-terpinene synthase
IUBMB Comments
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) , Citrus unshiu (satsuma) and Origanum vulgare (oregano) . Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to γ-terpinene.
The enzyme appears in viruses and cellular organisms
showSynonyms
gamma-terpinene synthase, tvtps, ovtps2, ostps24, clcts, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ClcTS
-
-
-
-
OvTPS2
-
-
-
-
TPS2
TvTPS2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = gamma-terpinene + diphosphate
-
-
-
-
geranyl diphosphate = gamma-terpinene + diphosphate
major products sabinene and gamma-terpinene are formed by a cyclization mechanism involving a 1,2-hydride shift in the alpha-terpinyl cation intermediate
geranyl diphosphate = gamma-terpinene + diphosphate
ionization of geranyl diphosphate folded in left-handed helical form gives, by syn-isomerization, (3R)-linalyl diphosphate, which by rotation about C2-C3 provides the cisoid, anti-endo conformation. Ionization and cyclization of this bound intermediate yield the (4R)-alpha-terpinyl cation that, following the 1,2-hydride shift, affords the terpinen-4-yl cation. Loss of the (5S)-proton terminates the reaction to generate gamma-terpinene
-
geranyl diphosphate = gamma-terpinene + diphosphate
mechanism involves the cyclizazion of the acyclic precursor, loss of a proton from C5 to form the delta4 double bond, and a 1,2-hydride shift fom C4 to C8 to give gamma-terpinene
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase (cyclizing, gamma-terpinene-forming)
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) [3], Citrus unshiu (satsuma) [4] and Origanum vulgare (oregano) [5]. Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
68943-55-5
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + H2O
gamma-terpinene + diphosphate
Substrates: -
Products: 63% sabinene, 21% gamma-terpinene, 7% terpinolene, 6.5% limonene, and 2.5% myrcene
Products: 63% sabinene, 21% gamma-terpinene, 7% terpinolene, 6.5% limonene, and 2.5% myrcene
?
neryl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: 75% gamma-terpinene, 20% alpha-thujene, 5% alpha-terpineol, partially purified enzyme
Products: 75% gamma-terpinene, 20% alpha-thujene, 5% alpha-terpineol, partially purified enzyme
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: plus small amounts of alpha-pinene, limonene
Products: plus small amounts of alpha-pinene, limonene
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: products are 71.4% gamma-terpinene, with lower amounts of limonene (9.1%), alpha-pinene (5.6%), beta-pinene (4.7%), alpha-terpinolene (3.7%), alpha-thujene (2.5%), alpha-terpinene (1.7%), myrcene (0.9%), sabinene (0.4%) and a trace of p-cymene
Products: products are 71.4% gamma-terpinene, with lower amounts of limonene (9.1%), alpha-pinene (5.6%), beta-pinene (4.7%), alpha-terpinolene (3.7%), alpha-thujene (2.5%), alpha-terpinene (1.7%), myrcene (0.9%), sabinene (0.4%) and a trace of p-cymene
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: products are 80% gamma-terpinene and the minor products, alpha-thujene (7.2%), alpha-terpinene (6%), myrcene (2.6%), sabinene (1.3%), (+)-R-limonene (1.2%), alpha-pinene (1%) and alpha-phellandrene (0.8%), as well as trace amounts of p-cymene and beta-pinene
Products: products are 80% gamma-terpinene and the minor products, alpha-thujene (7.2%), alpha-terpinene (6%), myrcene (2.6%), sabinene (1.3%), (+)-R-limonene (1.2%), alpha-pinene (1%) and alpha-phellandrene (0.8%), as well as trace amounts of p-cymene and beta-pinene
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
R4JHV6
Substrates: -
Products: plus minor products such as alpha-terpinene and alpha-thujene
Products: plus minor products such as alpha-terpinene and alpha-thujene
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: plus minor products alpha-thujene, alpha-pinene, sabinene, beta-myrcene, alpha-terpinene
Products: plus minor products alpha-thujene, alpha-pinene, sabinene, beta-myrcene, alpha-terpinene
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: ionization of geranyl diphosphate folded in left-handed helical form gives, by syn-isomerization, (3R)-linalyl diphosphate, which by rotation about C2-C3 provides the cisoid, anti-endo conformation. Ionization and cyclization of this bound intermediate yield the (4R)-alpha-terpinyl cation that, following the 1,2-hydride shift, affords the terpinen-4-yl cation. Loss of the (5S)-proton terminates the reaction to generate gamma-terpinene
Products: ionization of geranyl diphosphate folded in left-handed helical form gives, by syn-isomerization, (3R)-linalyl diphosphate, which by rotation about C2-C3 provides the cisoid, anti-endo conformation. Ionization and cyclization of this bound intermediate yield the (4R)-alpha-terpinyl cation that, following the 1,2-hydride shift, affords the terpinen-4-yl cation. Loss of the (5S)-proton terminates the reaction to generate gamma-terpinene
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: products are about 64% gamma-terpinen, 10% alpha-thujene, 4% myrcene, 4% alpha-terpinene, 2% limonene, 3% linalool, 2% terpinen-4-ol, 3% alpha-terpineol
Products: products are about 64% gamma-terpinen, 10% alpha-thujene, 4% myrcene, 4% alpha-terpinene, 2% limonene, 3% linalool, 2% terpinen-4-ol, 3% alpha-terpineol
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: product identification and quantification by GC-MS analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: product identification and quantification by GC-MS analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: GC-MS analysis of reaction product
Products: -
Products: -
?
additional information
?
-
Substrates: GC-MS analysis of reaction product
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate farnesyl diphosphate
Products: -
Products: -
?
additional information
?
-
Substrates: product identification and quantification by GC-MS analysis
Products: -
Products: -
?
additional information
?
-
Substrates: product identification and quantification by GC-MS analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: product identification and quantification by GC-MS analysis
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
gamma-terpinene + diphosphate
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Mg2+
or Mn2+, required. Optimum concentration of Mg2+ 0.025 mM, 44% of the activity wit Mn2+
Mg2+
required, essential for enzyme activity, best at 0.1 g/l during fermentation
Mn2+
or Mg2+, Mn2+ is preferred over Mg2+, optimum concentration 0.6 mM
Mn2+
or Mg2+, required. Mn2+ is preferred over Mg2+, optimum concentration 0.001 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Dehydration
Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8
7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
six different variants of the gene are distinguishable according to sequence characteristics in intron 3, after comprehensive analysis in Origanum dubium, Origanum majorana, Origanum onites, and Origanum syriacum. The formation of characteristic essential oil compounds is associated with the presence of variants 1 and/or 3 and/or 4 in the gene. In plants lacking these variants, the compounds are completely absent or present in trace amounts only
UniProt
brenda
carvacrol-rich plant, cloning from chemically distinct Thymus caespititius plants
R4JHV6
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
-
GC-MS analysis of plant volatiles st different developmental plant stages and varieties, overview
brenda
additional information
GC-MS analysis of plant volatiles at different developmental plant stages and varieties, overview
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
in general, monoterpene synthases contain a transit peptide for translocation to plastids and sesquiterpene synthases are localized in the cytoplasm
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
gamma-terpinene synthase (TPS) belongs to the monoterpene cyclases family
metabolism
-
the enzyme catalyzes the proposed first step of thymoquinone biosynthesis, the formation of gamma-terpinene from geranyl diphosphate (GDP)
physiological function
additional information
physiological function
The biosynthesis of gamma-terpinene, a precursor of the phenolic isomers thymol and carvacrol found in the essential oil from Thymus sp., is attributed to the activitiy of gamma-terpinene synthase (TvTPS)
physiological function
-
enzyme OsTPS24 is localized in chloroplasts and produced the monoterpene, gamma-terpinene. The amount of gamma-terpinene increased after jasmonate treatment. gamma-Terpinene has significant antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo), but it does not show significant antifungal activity against Magnaporthe oryzae. The antibacterial activity of gamma-terpinene against Xoo is caused by damage to bacterial cell membranes. These results suggest that gamma-terpinene plays an important role in jasmonate-induced resistance against Xoo, and that it functions as an antibacterial compound in rice plants
physiological function
the monoterpene synthase activity is predominantly regulated at the level of transcription, and the phenolic monoterpene alcohol thymol is derived from gamma-terpinene, the product of a single monoterpene synthase. Terpene synthase expression levels directly control the composition of the essential oils. Proportion (%) changes of essential oil components in different varieties of Origanum vulgare, GC-MS analysis of plant volatiles, overview
physiological function
-
the monoterpene synthase activity is predominantly regulated at the level of transcription, and the phenolic monoterpene alcohol thymol is derived from gamma-terpinene, the product of a single monoterpene synthase. Terpene synthase expression levels directly control the composition of the essential oils. Proportion (%) changes of essential oil components in different varieties of Origanum majorana, GC-MS analysis of plant volatiles, overview
physiological function
-
enzyme NsTPS1 elucidates the first dedicated step in the biosynthesis of thymoquinone in Nigella sativa
physiological function
gamma-terpinene is not only a monoterpene with antioxidant and antibacterial activities but also acts as a precursor of p-cymene, carvacrol, and thymol. In addition, gamma-terpinene synthase plays an essential role in the conversion of terpinen-4-yl cation to gamma-terpinene. Silver nanoparticles (AgNPs) strongly affect the secondary metabolites in cell suspension cultures of Carum carvi. 50 mg/ml AgNPs increase p-cymene and carvone contents in comparison with the control. The exposure of plants to 100 mg/l AgNPs induces the production of thymol and carvacrol. The application of AgNPs as an elicitor for 24 and 48 h significantly improves the essential oil composition and TPS2 gene expression in Carum carvi, overview
physiological function
the enzyme is responsible for the conversion of GPP into gamma-terpinene
additional information
putative active site of TvTPS is deduced from its pronounced structural similarity to enzymes from other species of the Lamiaceae family involved in terpenoid biosynthesis
additional information
putative active site of TvTPS is deduced from its pronounced structural similarity to enzymes from other species of the Lamiaceae family involved in terpenoid biosynthesis
additional information
-
putative active site of TvTPS is deduced from its pronounced structural similarity to enzymes from other species of the Lamiaceae family involved in terpenoid biosynthesis
additional information
tissue-specific expression patterns of genes associated with the terpeniod biosynthesis in two oregano species Origanum vulgare and Origanum majorana, GC-MS analysis of plant volatiles, overview
additional information
-
tissue-specific expression patterns of genes associated with the terpeniod biosynthesis in two oregano species Origanum vulgare and Origanum majorana, GC-MS analysis of plant volatiles, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GTPS1_THYVU
542
0
63274
Swiss-Prot
other Location (Reliability: 2)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
96000
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
dimer
overall structure of each TvTPS1 monomer comprises two alpha-helical domains: an N-terminal domain comprising an alpha/alpha barrel and a C-terminal orthogonal bundle domain, crystal structure analysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modeling based on gamma-terpinene synthase from Origanum vulgare. Besides the aspartate-rich region DDxxD, the sites Asp452, Thr456 and Glu460 involved on stabilization of active site by Mg2+ cofactor are also conserved
R4JHV6
purified recombinant enzyme, sitting drop method, mixing of 400-600 nl of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.8, 1 mM MgCl2, 4 mM DTT, with reservoir solution containing 0.2 M ammonium tartrate, pH 7.0, 20% w/v PEG 3350, in a 1:1 or 1:2 ratio, equilibration against 0.07 ml reservoir solution, 19°C, X-ray diffraction structure determination and analysis at 1.65-1.90 A resolution, two molecules (monomers A and B) in the asymmetric unit, molecular replacement using the crystal structure of (4S)-limonene synthase from Mentha spicata, PDB ID 2ong, model building
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D356A
site-directed mutagenesis, the activity of the mutant is completely abolished
T565A
site-directed mutagenesis, the mutation results in a 82% decrease in activity compared to wild-type
additional information
additional information
the exposure of plants to 100 mg/l silver nanoparticcles (AgNPs) causes a significant upregulation of CcTPS2 expression for 24 h. These cell suspension cultures are elicited by AgNPs, the application of which proves as an effective method to improve the production of secondary metabolites in vitro
additional information
-
the enzyme is a target for metabolic engineering for high-level thymoquinone production
additional information
production of gamma-terpinene by metabolically engineered Escherichia coli using glycerol as feedstock. The microbial production of GPP is available in Escherichia coli.Introduction of a heterologous mevalonate (MVA) pathway combined with the geranyl diphosphate synthase gene and gamma-terpinene synthase gene in to Escherichia coli, optimization of conditions to contain a titer of 19.42 mg/l. Finally, the fed-batch fermentation of gamma-terpineni is evaluated, where a maximum concentration of 275.41 mg/l with a maintainable feedstock of glycerol is achieved. Method optimization, detailed overview. Glycerol is the better carbon source during fermentation compared to glucose
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of either 20% glycerol or 1 mM substrate does not significantly improve stability
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 40 mM sodium phosphate, 5 mM MgCl2, 0.5 mM dithioerythritol, loss of 60% of activity within 1 week
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence from Escherichia coli by nickel affinity chromatography and dialysis
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds
gene gTRPS, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis
gene OsTPS24, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, functional recombinant expression of His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence in Escherichia coli, expression of GFP-tagged enzyme in Oryza sativa protoplasts chloroplasts
-
gene TPS1, DNA and amino acid sequence determination and analysis, codon-optimized gene, quantitative real-time PCR enzyme expression analysis, recombinant enzyme expression in Escherichia coli strains BL21(DE3) and CIBTS1756
gene TvTPS, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 CodonPlus
NsTPS1, cloning from seeds and functional recombinant expression in Escherichia coli
-
real-time PCR enzyme expression analysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
silver nanoparticles (AgNPs) the exposure of plants to 100 mg/l silver nanoparticcles (AgNPs) causes a significant upregulation of CcTPS2 expression for 24 h. These cell suspension cultures are elicited by AgNPs, the application of which proves as an effective method to improve the production of secondary metabolites in vitro
terpene synthase expression levels directly control the composition of the essential oil
the enzyme encoded by gene OsTPS24 is highly induced about 20fold by jasmonate, expression pattern, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
recombinant expression in Aspergillus nidulans resulting in production of gamma-terpinene. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds, e.g. gamma-terpinene
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wise, M.L.; Savage, T.J.; Katahira, E.; Croteau, R.
Monoterpene synthases from common sage (Salvia officinalis). cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1,8-cineole synthase, and (+)-bornyl diphosphate synthase
J. Biol. Chem.
273
14891-14899
1998
Salvia officinalis (O81193)
brenda
Lucker, J.; El Tamer, M.K.; Schwab, W.; Verstappen, F.W.; van der Plas, L.H.; Bouwmeester, H.J.; Verhoeven, H.A.
Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases
Eur. J. Biochem.
269
3160-3171
2000
Citrus x limon (Q8L5K4)
brenda
LaFever, R.E.; Croteau, R.
Hydride shifts in the biosynthesis of the p-menthane monoterpenes alpha-terpinene, gamma-terpinene, and beta-phellandrene
Arch. Biochem. Biophys.
301
361-366
1993
Thymus vulgaris
brenda
Poulose, A.; Croteau, R.
gamma-Terpinene synthetase: A key enzyme in the biosynthesis of aromatic monoterpenes
Arch. Biochem. Biophys.
191
400-411
1978
Thymus vulgaris
brenda
Alonso, W.R.; Croteau, R.
Purification and characterization of the monoterpene cyclase gamma-terpinene synthase from Thymus vulgaris
Arch. Biochem. Biophys.
286
511-517
1991
Thymus vulgaris
brenda
Suzuki, Y.; Sakai, H.; Shimada, T.; Omura, M.; Kumazawa, S.; Nakayama, T.
Characterization of gamma-terpinene synthase from Citrus unshiu (Satsuma mandarin)
Biofactors
21
79-82
2004
Citrus unshiu (Q6F5H1), Citrus unshiu
brenda
Lukas, B.; Samuel, R.; Novak, J.
Oregano or marjoram? The enzyme gamma-terpinene synthase affects chemotype formation in the genus Origanum
Isr. J. Plant Sci.
58
211-220
2010
Origanum syriacum (G3LTY3)
-
brenda
Crocoll, C.; Asbach, J.; Novak, J.; Gershenzon, J.; Degenhardt, J.
Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis
Plant Mol. Biol.
73
587-603
2010
Origanum vulgare (E2E2P0)
brenda
Mendes, M.D.; Barroso, J.G.; Oliveira, M.M.; Trindade, H.
Identification and characterization of a second isogene encoding alpha-terpinene synthase in Thymus caespititius
J. Plant Physiol.
171
1017-1027
2014
Thymus caespititius (A0A068EN29)
brenda
Lima, A.S.; Schimmel, J.; Lukas, B.; Novak, J.; Barroso, J.G.; Figueiredo, A.C.; Pedro, L.G.; Degenhardt, J.; Trindade, H.
Genomic characterization, molecular cloning and expression analysis of two terpene synthases from Thymus caespititius (Lamiaceae)
Planta
238
191-204
2013
Thymus caespititius (R4JHV6)
brenda
Rudolph, K.; Parthier, C.; Egerer-Sieber, C.; Geiger, D.; Muller, Y.A.; Kreis, W.; Mueller-Uri, F.
Expression, crystallization and structure elucidation of gamma-terpinene synthase from Thymus vulgaris
Acta Crystallogr. Sect. F
72
16-23
2016
Thymus vulgaris (A0A0M3Q1Q3), Thymus vulgaris (K9Y6Y9), Thymus vulgaris
brenda
Bromann, K.; Toivari, M.; Viljanen, K.; Ruohonen, L.; Nakari-Setaelae, T.
Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-terpinene production
Appl. Microbiol. Biotechnol.
100
6345-6359
2016
Citrus unshiu (Q6F5H0), Citrus unshiu
brenda
Jan, S.; Mir, J.; Shafi, W.; Faktoo, S.; Singh, D.; Wijaya, L.; Alyemeni, M.; Ahmad, P.
Divergence in tissue-specific expression patterns of genes associated with the terpeniod biosynthesis in two oregano species Origanum vulgare L., and Origanum majorana
Indust. Crops Prod.
123
546-555
2018
Origanum vulgare (E2E2P0), Origanum majorana
-
brenda
Yoshitomi, K.; Taniguchi, S.; Tanaka, K.; Uji, Y.; Akimitsu, K.; Gomi, K.
Rice terpene synthase 24 (OsTPS24) encodes a jasmonate-responsive monoterpene synthase that produces an antibacterial gamma-terpinene against rice pathogen
J. Plant Physiol.
191
120-126
2016
Oryza sativa
brenda
Dehghani-Aghchekohal, Z.; Omidi, M.; Azizinezhad, R.; Etminan, A.
Stimulation of secondary metabolites and gamma-terpinene synthase by silver nanoparticles in callus cultures of Carum carvi
Appl. Biochem. Biotechnol.
194
3228-3241
2022
Carum carvi (A0A8A9RYM4)
brenda
Elyasi, R.; Majdi, M.; Krause, S.T.; Kuecuekay, N.; Azizi, A.; Degenhardt, J.
Identification and functional characterization of a gamma-terpinene synthase in Nigella sativa L (black cumin)
Phytochemistry
202
113290
2022
Nigella sativa
brenda
Qi, C.; Zhao, H.; Li, W.; Li, X.; Xiang, H.; Zhang, G.; Liu, H.; Wang, Q.; Wang, Y.; Xian, M.; Zhang, H.
Production of gamma-terpinene by metabolically engineered Escherichia coli using glycerol as feedstock
RSC Adv.
8
30851-30859
2018
Thymus vulgaris (A0A0M3Q1Q3)
brenda
EXTERNAL LINKS (specific for EC-Number 4.2.3.114)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
