Literature summary for 4.2.2.17 extracted from

Yu, S.; Zhang, Y.; Zhu, Y.; Zhang, T.; Jiang, B.; Mu, W.
Improving the catalytic behavior of DFA I-forming inulin fructotransferase from Streptomyces davawensis with site-directed mutagenesis (2017), J. Agric. Food Chem., 65, 7579-7587 .

Search for more literature for 4.2.2.17

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces davaonensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure	Streptomyces davaonensis

Protein Variants

Protein Variants	Comment	Organism
A257S	mutation of residue in central tunnel, no significant change of temperature stability	Streptomyces davaonensis
A257S/G281S G281S	mutation of residues in central tunnel, increase in temperature stability by 3 degrees	Streptomyces davaonensis
A257T	mutation of residue in central tunnel, 2.8 degrees decrease of temperature stability	Streptomyces davaonensis
G121A/T122L	148.7% of wild-type activity, about 4.5 degrees increase in temperature stability	Streptomyces davaonensis
G236S/G281S/A257S/T313S/A314S	140.5% of wild-type activity, increase in temperature stability by 3.5 degrees	Streptomyces davaonensis
G281S	mutation of residue in central tunnel, 76% of wild-type activity, increase in temperature stability by 3 degrees	Streptomyces davaonensis
G281T	mutation of residue in central tunnel, no significant change of temperature stability	Streptomyces davaonensis
I80A	11.6% of wild-type activity, about 2 degrees increase in temperature stability	Streptomyces davaonensis
I80E	15.7% of wild-type activity, about 1.5 degrees increase in temperature stability	Streptomyces davaonensis
I80S	41.4% of wild-type activity, about 1 degrees increase in temperature stability	Streptomyces davaonensis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces davaonensis	K4QSF6	-	-

Synonyms

Synonyms	Comment	Organism
IFTase	-	Streptomyces davaonensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
72.8	-	melting temperature of mutant A257T	Streptomyces davaonensis
75.6	-	melting temperature of wild-type and mutant A257S	Streptomyces davaonensis
78.8	-	melting temperature of mutant G236S/G281S/A257S/T313S/A314S	Streptomyces davaonensis
80.1	-	melting temperature of mutant G121A/T122L	Streptomyces davaonensis

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Release 2023.1 (January 2023)