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beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
additional information
?
-
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
Substrates: -
Products: -
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
Substrates: -
Products: -
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
Substrates: -
Products: -
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
Substrates: -
Products: -
?
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
Substrates: -
Products: -
?
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
Substrates: -
Products: -
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: -
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: -
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
Substrates: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: 1-kestose
Products: -
?
additional information
?
-
-
Substrates: no substrate: 1-kestose
Products: -
?
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A257S
mutation of residue in central tunnel, no significant change of temperature stability
A257S/G281S G281S
mutation of residues in central tunnel, increase in temperature stability by 3 degrees
A257T
mutation of residue in central tunnel, 2.8 degrees decrease of temperature stability
D183A
-
complete loss of activity
D183N
-
complete loss of activity
E194A
-
complete loss of activity
E194N
-
complete loss of activity
G121A/T122L
148.7% of wild-type activity, about 4.5 degrees increase in temperature stability
G236S/G281S/A257S/T313S/A314S
140.5% of wild-type activity, increase in temperature stability by 3.5 degrees
G281S
mutation of residue in central tunnel, 76% of wild-type activity, increase in temperature stability by 3 degrees
G281T
mutation of residue in central tunnel, no significant change of temperature stability
I80A
11.6% of wild-type activity, about 2 degrees increase in temperature stability
I80E
15.7% of wild-type activity, about 1.5 degrees increase in temperature stability
I80S
41.4% of wild-type activity, about 1 degrees increase in temperature stability
D183A
-
complete loss of activity
-
D183N
-
complete loss of activity
-
E194A
-
complete loss of activity
-
E194N
-
complete loss of activity
-
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