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IUBMB CommentsThis enzyme, like EC 4.2.2.16 [levan fructotransferase (DFA-IV-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
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beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
additional information
?
-
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside

di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
fructofuranosyl nystose

di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
-
?
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
-
?
inulin

3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
-
-
?
inulin

di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
-
-
-
?
inulin

di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
nystose

di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
-
?
additional information

?
-
-
no substrate: 1-kestose
-
-
?
additional information
?
-
-
no substrate: 1-kestose
-
-
?
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inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
inulin

3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
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?
x * 41422, calculated from sequence, x * 41000, SDS-PAGE
dimer

-
2 * 38000, SDS-PAGE
dimer
-
2 * 37000, SDS-PAGE
dimer
-
2 * 38000, SDS-PAGE
-
dimer
-
2 * 37000, SDS-PAGE
-
monomer

-
1 * 40000, gel filtration
monomer
-
1 * 46000, gel filtration
monomer
-
1 * 46000, gel filtration
-
monomer
-
1 * 40000, gel filtration
-
monomer
1 * 41499, calculated from sequence, 1 * 41000, SDS-PAGE
monomer
1 * 41971, calculated from sequence, 1 * 42000, SDS-PAGE
monomer
-
1 * 41971, calculated from sequence, 1 * 42000, SDS-PAGE
-
trimer

-
3 * 40000, SDS-PAGE, 3 * 40769, calculated from sequence
trimer
-
3 * 40000, SDS-PAGE, 3 * 40769, calculated from sequence
-
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A257S
mutation of residue in central tunnel, no significant change of temperature stability
A257S/G281S G281S
mutation of residues in central tunnel, increase in temperature stability by 3 degrees
A257T
mutation of residue in central tunnel, 2.8 degrees decrease of temperature stability
D183A
-
complete loss of activity
D183N
-
complete loss of activity
E194A
-
complete loss of activity
E194N
-
complete loss of activity
G121A/T122L
148.7% of wild-type activity, about 4.5 degrees increase in temperature stability
G236S/G281S/A257S/T313S/A314S
140.5% of wild-type activity, increase in temperature stability by 3.5 degrees
G281S
mutation of residue in central tunnel, 76% of wild-type activity, increase in temperature stability by 3 degrees
G281T
mutation of residue in central tunnel, no significant change of temperature stability
I80A
11.6% of wild-type activity, about 2 degrees increase in temperature stability
I80E
15.7% of wild-type activity, about 1.5 degrees increase in temperature stability
I80S
41.4% of wild-type activity, about 1 degrees increase in temperature stability
D183A
-
complete loss of activity
-
D183N
-
complete loss of activity
-
E194A
-
complete loss of activity
-
E194N
-
complete loss of activity
-
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