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Information on EC 4.2.2.17 - inulin fructotransferase (DFA-I-forming)
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EC Tree
4.2.2.17 inulin fructotransferase (DFA-I-forming)IUBMB Comments
This enzyme, like EC 4.2.2.16 [levan fructotransferase (DFA-IV-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
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Word Map
- 4.2.2.17
- arthrobacter
- difructose
-
sweetener
- globiformis
-
2.4.1.93
-
iii-producing
- nystose
-
low-calorie
-
smallest
- aurescens
-
hydrostatic
-
ultrafiltration
- methanol
- pastoris
- fructooligosaccharides
- broth
-
pichia
- nutrition
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
iftase, inulin fructotransferase (dfa i-producing), 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
EC 2.4.1.200
-
-
formerly
-
inulin D-fructosyl-D-fructosyltransferase (1,2':2',1-dianhydride-forming)
-
-
-
-
inulin fructotransferase (depolymerizing, difructofuranose-1,2':2',1-dianhydride-forming)
-
-
-
-
inulin fructotransferase (DFA I-producing)
inulin fructotransferase (DFA-I-producing)
-
-
-
-
Noca_0068
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[inulin]n = [inulin]n-2 + alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D-fructosyl-D-fructofuranosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
(2->1)-beta-D-fructan lyase (alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,1'-dianhydride-forming)
This enzyme, like EC 4.2.2.16 [levan fructotransferase (DFA-IV-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
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CAS REGISTRY NUMBER
COMMENTARY
125008-19-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
?
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
-
-
-
?
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
Streptomyces davaonensis SK39.001
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
Streptomyces davaonensis SK39.001
-
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
-
-
-
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
Streptomyces davaonensis SK39.001
-
-
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
-
i.e. DFA I
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 8
-
10% of maximal activity at pH 3.5, 25% of maximal activity at pH 8.0
4.5 - 7
-
pH 4.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
45
50
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
80% of maximal activity at 30°C, 20% of maximal activity at 65°C
30 - 60
-
30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). K4QSF6_STRDJ
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
392
0
41834
TrEMBL
-
B8HEC1_PSECP
Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6)
444
0
46997
TrEMBL
-
F9EF46_9ACTO
393
0
41999
TrEMBL
-
C6B4D5_RHILS
Rhizobium leguminosarum bv. trifolii (strain WSM1325)
457
0
49288
TrEMBL
-
B0E672_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
448
0
47471
TrEMBL
other Location (Reliability: 2)
B6A3D9_RHILW
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
453
0
48499
TrEMBL
-
A0A7U9GVM9_KLEPN
457
0
49790
TrEMBL
-
A1SCS9_NOCSJ
Nocardioides sp. (strain ATCC BAA-499 / JS614)
392
0
41971
TrEMBL
-
A0A840PHE3_9ACTN
441
1
47256
TrEMBL
-
A0A2N9B0A9_STRCX
393
0
42034
TrEMBL
-
B8HDZ1_PSECP
Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6)
445
0
47152
TrEMBL
-
A0A494WMI6_CLOS5
Clostridium scindens (strain ATCC 35704 / DSM 5676 / VPI 13733 / 19)
80
0
8771
TrEMBL
-
B0EUB5_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
448
0
47485
TrEMBL
other Location (Reliability: 2)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
monomer
trimer
monomer
1 * 41499, calculated from sequence, 1 * 41000, SDS-PAGE
trimer
Streptomyces davaonensis SK39.001
-
3 * 40000, SDS-PAGE, 3 * 40769, calculated from sequence
-
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modelling and molecular docking. Residues D183 and E194 are potential catalytic residues
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A257S
mutation of residue in central tunnel, no significant change of temperature stability
A257S/G281S G281S
mutation of residues in central tunnel, increase in temperature stability by 3 degrees
A257T
mutation of residue in central tunnel, 2.8 degrees decrease of temperature stability
G121A/T122L
148.7% of wild-type activity, about 4.5 degrees increase in temperature stability
G236S/G281S/A257S/T313S/A314S
140.5% of wild-type activity, increase in temperature stability by 3.5 degrees
G281S
mutation of residue in central tunnel, 76% of wild-type activity, increase in temperature stability by 3 degrees
G281T
mutation of residue in central tunnel, no significant change of temperature stability
I80A
11.6% of wild-type activity, about 2 degrees increase in temperature stability
I80E
15.7% of wild-type activity, about 1.5 degrees increase in temperature stability
I80S
41.4% of wild-type activity, about 1 degrees increase in temperature stability
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
70