Cloned (Comment) | Organism |
---|---|
gene Hyl, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus suis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001 ml of protein in 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM mercaptoethanol, and 1 mM EDTA, is mixed with 0.001 ml of reservoir solution, and equilibrated against 0.25 ml of reservoir solution, a few weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution, molecular-replacement method | Streptococcus suis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus suis | - |
gene Hyl | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chomatography, dialysis, gel filtration and ultrafiltration | Streptococcus suis |