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Literature summary for 4.2.1.93 extracted from

  • Marbaix, A.Y.; Tyteca, D.; Niehaus, T.D.; Hanson, A.D.; Linster, C.L.; Van Schaftingen, E.
    Occurrence and subcellular distribution of the NADPHX repair system in mammals (2014), Biochem. J., 460, 49-58 .
    View publication on PubMed
Search for more literature for 4.2.1.93

Cloned(Commentary)

Cloned (Comment) Organism
gene Carkd, recombinant expression of Myc-His-tagged mitochondrial isozyme mCarkd, the enzyme contains a mitochondrial propeptide, in HEK-293T cells, the recombinant enzyme is not secreted (not due to dysfunction of the HEK-293T cells) Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cCARKD Mus musculus 5829
-
endoplasmic reticulum spCARKD Mus musculus 5783
-
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion mCARKD Mus musculus 5739
-
additional information subcellular localization study of Myc-tagged enzyme, the Carkd gene encodes proteins with a predicted mitochondrial propeptide (mCARKD), a signal peptide (spCARKD) or neither of them (cCARKD) Mus musculus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Mus musculus
-
 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Rattus norvegicus
-
 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Mus musculus
-
 ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Rattus norvegicus
-
 ADP + phosphate + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9CZ42
-
-
Rattus norvegicus D4AAT7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein spCARKD comprises two putative N-glycosylation sites at positions 236-238 (236NIT238) and 293-295 (293NGS295) in the mouse protein, deglycosylation by PNGase F Mus musculus
no glycoprotein cCARKD is not glycosylated Mus musculus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from rat liver mitochondria by anion exchange chromatography, solubilization with 0.1% Triton X-100, desalting gel filtration, and Blue Trisacryl affinity chromatography Rattus norvegicus
recombinant Myc-His-tagged mCarkd from HEK-293T cells by affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
heart lowest enzyme content Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Mus musculus
-
liver highest enzyme content Rattus norvegicus
-
lung
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Mus musculus ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Rattus norvegicus ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Mus musculus ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Rattus norvegicus ADP + phosphate + NADPH
-
 ?
additional information NAD(P)HX dehydratase is specific for the (S)-epimer of NAD(P)HX Mus musculus ?
-
 ?
additional information NAD(P)HX dehydratase is specific for the (S)-epimer of NAD(P)HX Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, glycosylated spCARKD, SDS-PAGE, x * 38000, deglycosylated spCARKD, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
Carkd
-
 Mus musculus
Carkd
-
 Rattus norvegicus
NAD(P)HX dehydratase
-
 Mus musculus
NAD(P)HX dehydratase
-
 Rattus norvegicus
NAXD
-
 Mus musculus
NAXD
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Mus musculus
ATP dependent on Rattus norvegicus

General Information

General Information Comment Organism
metabolism hydration of NAD(P)H to NAD(P)HX, which inhibits several dehydrogenases, is corrected by an ATP-dependent dehydratase and an epimerase recently identified as the products of the vertebrate Carkd (carbohydrate kinase domain) and Aibp (apolipoprotein AI-binding protein) genes respectively. The NAD(P)HX epimerase, encoded by the Aibp gene, catalyses the R to S epimerization of NAD(P)HX Mus musculus
metabolism hydration of NAD(P)H to NAD(P)HX, which inhibits several dehydrogenases, is corrected by an ATP-dependent dehydratase and an epimerase recently identified as the products of the vertebrate Carkd (carbohydrate kinase domain) and Aibp (apolipoprotein AI-binding protein) genes respectively. The NAD(P)HX epimerase, encoded by the Aibp gene, catalyses the R to S epimerization of NAD(P)HX Rattus norvegicus
physiological function the enzyme is part of the NADPHX repair system catalyzing hydration of inhibitory NAD(P)HX to the enzyme cofactor NAD(P)H Mus musculus
physiological function the enzyme is part of the NADPHX repair system catalyzing hydration of inhibitory NAD(P)HX to the enzyme cofactor NAD(P)H Rattus norvegicus