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Information on EC 4.2.1.93 - ATP-dependent NAD(P)H-hydrate dehydratase for references in articles please use BRENDA:EC4.2.1.93Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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ATP-dependent NAD(P)H-hydrate dehydratase
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ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH
also acts on hydrated NADPH
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ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH
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C-O bond cleavage by elimination of water
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(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase (ATP-hydrolysing; NADH-forming)
Acts equally well on hydrated NADH and hydrated NADPH. NAD(P)H spontaneously hydrates to both the (6S)- and (6R)- isomers, and these are interconverted by EC 5.1.99.6, NAD(P)H-hydrate epimerase, to a 60:40 ratio [4]. Hence EC 4.2.1.93 together with EC 5.1.99.6 can restore the mixture of hydrates into NAD(P)H [3,4]. The enzyme from eukaryotes has no activity with ADP, contrary to the enzyme from bacteria (cf. EC 4.2.1.136, ADP-dependent NAD(P)H-hydrate dehydratase) [4].
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ATP-dependent H4NAD(P)OH dehydratase
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reduced nicotinamide adenine dinucleotide hydrate dehydratase
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(S)-NAD(P)HX dehydratase
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(S)-NAD(P)HX dehydratase
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(S)-NAD(P)HX dehydratase
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(S)-NAD(P)HX dehydratase
K7TRV3;
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ATP-dependent NNRD
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NAD(P)X dehydratase
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NNRD
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gene At5g19150
UniProt
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gene At5g19150
UniProt
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yeast
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K7TRV3
UniProt
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evolution
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domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants
evolution
K7TRV3;
domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants
evolution
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domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants
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evolution
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domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants
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metabolism
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the stereospecific dehydratase is involved in a potential NAD(P)H repair pathway in plants. Dehydratase NNRD and an epimerase (NNRE), fused to a vitamin B6 salvage enzyme, act concomitantly to restore NAD(P)HX to NAD(P)H, but the proteins do not physically interact
metabolism
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the stereospecific dehydratase is involved in a potential NAD(P)H repair pathway in plants. Dehydratase NNRD and an epimerase (NNRE), fused to a vitamin B6 salvage enzyme, act concomitantly to restore NAD(P)HX to NAD(P)H, but the proteins do not physically interact
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physiological function
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the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites
physiological function
K7TRV3;
the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites
physiological function
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the enzyme is involved in the NAD(P)H repair pathway but is dispensable for growth and development under standard conditions
physiological function
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the enzyme is involved in the NAD(P)H repair pathway but is dispensable for growth and development under standard conditions; the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites
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physiological function
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the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites
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additional information
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homology models of the three-dimensional structures of NAD(P)X dehydratase and NAD(P)X epimerase
additional information
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homology models of the three-dimensional structures of NAD(P)X dehydratase and NAD(P)X epimerase
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ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
additional information
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no reaction occurs in absence of ATP, AMP is completely inactive, with ADP an initial lag period is observed, but the reaction then proceeds to completion, ADP appears to be active as source of ATP
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ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
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?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
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stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
stereospecific for (S)-NAD(P)HX epimer
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
-
-
-
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?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
-
stereospecific for (S)-NAD(P)HX epimer
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
-
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
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-
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ir
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
K7TRV3;
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
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-
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
stereospecific for (S)-NAD(P)HX epimer
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
-
stereospecific for (S)-NAD(P)HX epimer
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
stereospecific for (S)-NAD(P)HX epimer
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
-
-
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
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stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
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stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
K7TRV3;
stereospecific for (S)-NAD(P)HX epimer
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-
?
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ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
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?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
Q94AF2
stereospecific for (S)-NAD(P)HX epimer
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?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
Q94AF2
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
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-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
-
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
K7TRV3
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
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?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
Q94AF2
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
Q94AF2
stereospecific for (S)-NAD(P)HX epimer
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-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
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-
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
-
stereospecific for (S)-NAD(P)HX epimer
-
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
K7TRV3
stereospecific for (S)-NAD(P)HX epimer
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?
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ATP
K7TRV3;
dependent on
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additional information
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Ca2+ has no effect
Mg2+
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0.00086 - 0.00115
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
0.00069 - 0.00089
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
0.00086
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
K7TRV3;
pH 8.0, 22°C
0.00115
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
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pH 8.0, 22°C
0.00069
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
pH 8.0, 22°C
0.00089
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
K7TRV3;
pH 8.0, 22°C
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0.26 - 0.3
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
0.34 - 0.38
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
0.26
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
pH 8.0, 22°C
0.3
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
K7TRV3;
pH 8.0, 22°C
0.34
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
pH 8.0, 22°C
0.38
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
K7TRV3;
pH 8.0, 22°C
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262 - 358
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
509 - 524
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
262
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
pH 8.0, 22°C
358
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
K7TRV3;
pH 8.0, 22°C
509
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
pH 8.0, 22°C
524
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
K7TRV3;
pH 8.0, 22°C
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6
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assay at
8
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assay at
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22
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assay at
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weak expression
brenda
additional information
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tissue expression profile, overview
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rosette and cauline leaves, weak expression
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rosette and cauline leaves, weak expression
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weak expression
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weak expression
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dry, main expression
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dry, main expression
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weak expression
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; weak expression
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weak expression
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weak expression
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import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
brenda
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brenda
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; import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
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brenda
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import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
-
brenda
K7TRV3;
import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
brenda
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brenda
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brenda
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K7TRV3;
-
brenda
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import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
brenda
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brenda
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; import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
-
brenda
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import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
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brenda
K7TRV3;
import assays with purified pea (Pisum sativum) chloroplasts and mitochondria
brenda
additional information
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the enzyme is predominantly (if not exclusively) found in chloroplasts and mitochondria
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brenda
additional information
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the enzyme is predominantly (if not exclusively) found in chloroplasts and mitochondria
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brenda
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Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487);
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
J9VIT7
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487);
J9VIT7
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Q9X024
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
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35701
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4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering
132700
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about, gel filtration coupled to static light scattering
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tetramer
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4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering
tetramer
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4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering
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recombinant C-terminally His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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gene At5g19150, sequence comparisons, recombinant expression of C-terminally His6-tagged in Escherichia coli strain BL21(DE3), expression of YFP-tagged enzyme in Arabidopsis thaliana cells
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recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts
recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts
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recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts
K7TRV3;
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Meinhart, J.O.; Chaykin, S.; Krebs, E.G.
Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide
J. Biol. Chem.
220
821-829
1956
Saccharomyces cerevisiae
brenda
Acheson, S.A.; Kirkman, H.N.; Wolfenden, R.
Equilibrium of 5,6-hydration of NADH and mechanism of ATP-dependent dehydration
Biochemistry
27
7371-7375
1988
Saccharomyces cerevisiae
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Niehaus, T.D.; Richardson, L.G.; Gidda, S.K.; Elbadawi-Sidhu, M.; Meissen, J.K.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.
Plants utilize a highly conserved system for repair of NADH and NADPH hydrates
Plant Physiol.
165
52-61
2014
Arabidopsis thaliana (Q94AF2), Arabidopsis thaliana Col-0 (Q94AF2), Zea mays (K7TRV3)
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Colinas, M.; Shaw, H.; Loubery, S.; Kaufmann, M.; Moulin, M.; Fitzpatrick, T.
A pathway for repair of NAD(P)H in plants
J. Biol. Chem.
289
14692-14706
2014
Arabidopsis thaliana (Q94AF2), Arabidopsis thaliana Col-0 (Q94AF2)
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