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Literature summary for 4.2.1.9 extracted from
- Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice (2011), Microbiology, 157, 38-46.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | DHAD can be a potential drug/vaccine target, as it is absent in mammals | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Rv0189c or ilvD, overexpression of thioredoxin-His-tagged IlvD in Escherichia coli strain OrigamiBL21(DE3). Transcript levels of Rv0189c in sense and antisense transformants of Mycobacterium tuberculosis, phenotype, overview | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of strain BL21(DE3) DELTAilvD knockout mutant, functional complementation by Mycobacterium tuberculosis IlvD | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NO | inactivation of Rv0189c by NO probably inhibits bacterial growth | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.7 | - |
DL-2,3-dihydroxyisovalerate | pH 8.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78300 | - |
2 * 78300, recombinant thioredoxin-His-tagged IlvD, SDS-PAGE | Mycobacterium tuberculosis |
| 155000 | - |
gel filtration, recombinant thioredoxin-His-tagged IlvD | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene ilvD | - |
| Mycobacterium tuberculosis | - |
gene Rv0189c or ilvD | - |
| Mycobacterium tuberculosis H37Rv | - |
gene Rv0189c or ilvD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant thioredoxin-His-tagged IlvD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | upregulation of Rv0189c occurs during the early exponential phase of growth | Mycobacterium tuberculosis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.024 | - |
purified native enzyme, pH 8.0, temperature not specified in the publication | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-2,3-dihydroxyisovalerate | - |
Mycobacterium tuberculosis | ? | - |
? | |
| DL-2,3-dihydroxyisovalerate | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 78300, recombinant thioredoxin-His-tagged IlvD, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHAD | - |
Escherichia coli |
| DHAD | - |
Mycobacterium tuberculosis |
| dihydroxyacid dehydratase | - |
Escherichia coli |
| dihydroxyacid dehydratase | - |
Mycobacterium tuberculosis |
| IlvD | - |
Escherichia coli |
| IlvD | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mycobacterium tuberculosis |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mycobacterium tuberculosis | upregulation of Rv0189c occurs during the early exponential phase of growth, under acid stress and ex vivo. Rv0189c expression is upregulated by 1.72fold at pH 4.5 and 1.42fold at pH 5.5, compared to that at pH 7.2, relative gene expression profiles of Rv0189c starved in PBS, overview | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | dihydroxyacid dehydratase, a key enzyme involved in branched-chain amino acid biosynthesis, catalyses the synthesis of 2-oxoacids from dihydroxyacids | Escherichia coli |
| metabolism | dihydroxyacid dehydratase, a key enzyme involved in branched-chain amino acid biosynthesis, catalyses the synthesis of 2-oxoacids from dihydroxyacids | Mycobacterium tuberculosis |
| physiological function | Rv0189c has a role in the survival of Mycobacterium tuberculosis during normal and stress conditions | Mycobacterium tuberculosis |
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