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Literature summary for 4.2.1.9 extracted from
- Dihydroxy acid dehydratase from spinach contains a [2Fe-2S] cluster (1988), J. Biol. Chem., 263, 3558-3564.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
2,3-dihydroxyisovalerate | - |
Spinacia oleracea |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains a catalytically active [2Fe-2S] cluster | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
2 * 63000, SDS-PAGE | Spinacia oleracea |
| 105000 | - |
gel filtration | Spinacia oleracea |
| 110000 | - |
native PAGE | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Spinacia oleracea | third enzyme in the branched-chain amino acid biosynthetic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxy-3-methylbutanoate | - |
Spinacia oleracea | 2-oxoisovalerate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylpentanoate | - |
Spinacia oleracea | 3-methyl-2-oxopentanoate + H2O | - |
? | |
| additional information | third enzyme in the branched-chain amino acid biosynthetic pathway | Spinacia oleracea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 63000, SDS-PAGE | Spinacia oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25 | - |
2,3-dihydroxyisovalerate | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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