Any feedback?
Literature summary for 4.2.1.84 extracted from
- Enantioselective production of 2,2-dimethylcyclopropane carboxylic acid from 2,2-dimethylcyclopropane carbonitrile using the nitrile hydratase and amidase of Rhodococcus erythropolis ATCC 25544 (2007), Enzyme Microb. Technol., 41, 842-848.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | industrial production of (S)-2,2-dimethylcyclopropanecarboxylic acid | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas oleophilus | - |
strain ATCC 15963 | - |
| Nocardia transvalensis | - |
strain ATCC 12485 | - |
| Rhodococcus erythropolis | - |
strain ATCC 25544 | - |
| Rhodococcus rhodochrous | - |
strain ATCC 12674 | - |
| Rhodococcus rhodochrous | - |
strain ATCC 33278 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.078+/-0.003 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase | Nocardia transvalensis |
| additional information | - |
0.096 +/- 0.003 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropane carbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase | Rhodococcus erythropolis |
| additional information | - |
0.109 +/- 0.002 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase | Rhodococcus rhodochrous |
| additional information | - |
0.220 +/- 0.006 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase | Comamonas oleophilus |
| additional information | - |
0.438 +/- 0.01 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase | Rhodococcus rhodochrous |
| additional information | - |
optimization of reaction conditions (pH, temperature, organic solvent) for conversion of 2,2-dimethylcyclopropanecarbonitrile into (S)-2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase enzyme system | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2-dimethylcyclopropanecarbonitrile + H2O | - |
Rhodococcus rhodochrous | 2,2-dimethylcyclopropanecarboxamide | - |
? |  |
| 2,2-dimethylcyclopropanecarbonitrile + H2O | - |
Rhodococcus erythropolis | 2,2-dimethylcyclopropanecarboxamide | - |
? | |
| 2,2-dimethylcyclopropanecarbonitrile + H2O | - |
Nocardia transvalensis | 2,2-dimethylcyclopropanecarboxamide | - |
? | |
| 2,2-dimethylcyclopropanecarbonitrile + H2O | - |
Comamonas oleophilus | 2,2-dimethylcyclopropanecarboxamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrile hydratase | - |
Rhodococcus rhodochrous |
| nitrile hydratase | - |
Rhodococcus erythropolis |
| nitrile hydratase | - |
Nocardia transvalensis |
| nitrile hydratase | - |
Comamonas oleophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
substrate: 2,2-dimethylcyclopropanecarbonitrile | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
substrate: 2,2-dimethylcyclopropanecarbonitrile | Rhodococcus erythropolis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
