BRENDA - Enzyme Database show
show all sequences of 4.2.1.84

Characterization of a new cobalt-containing nitrile hydratase purified from urea-induced cells of Rhodococcus rhodochrous J1

Nagasawa, T.; Takeuchi, K.; Yamada, H.; Eur. J. Biochem. 196, 581-589 (1991)

Data extracted from this reference:

General Stability
General Stability
Organism
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
0.01-1 mM AgNO3
Rhodococcus rhodochrous
diethyldithiocarbamate
0.1 mM
Rhodococcus rhodochrous
Hg2+
HgCl2, 0.01-1 mM
Rhodococcus rhodochrous
hydroxylamine
1 mM
Rhodococcus rhodochrous
KCN
0.01 mM
Rhodococcus rhodochrous
Na2S2O4
1 mM
Rhodococcus rhodochrous
phenylhydrazine
1 mM
Rhodococcus rhodochrous
Semicarbazide
1 mM, weak inhibition
Rhodococcus rhodochrous
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.85
-
Isobutyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
1.89
-
acrylonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
1.92
-
Propionitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.88
-
Crotononitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
6.76
-
Methacrylonitrile
pH 7.0, 20ºC
Rhodococcus rhodochrous
10
-
Cyanopyrazine
pH 7.0, 20ºC
Rhodococcus rhodochrous
12.2
-
Chloroacetonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
18.5
-
4-cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
21.7
-
2-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
21.7
-
n-butyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
28.6
-
Benzonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
200
-
3-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
contains approximately 11-12 mol cobalt/mol enzyme
Rhodococcus rhodochrous
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
29000
-
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
505000
530000
sedimentation equilibrium method, gel permeation HPLC
Rhodococcus rhodochrous
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus rhodochrous
-
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase
-
Rhodococcus rhodochrous J1
-
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase
-
Purification (Commentary)
Commentary
Organism
; ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rhodococcus rhodochrous
371
-
purified enzyme
Rhodococcus rhodochrous
Storage Stability
Storage Stability
Organism
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyanopyridine + H2O
-
648439
Rhodococcus rhodochrous
pyridine-2-carbamide
-
-
-
?
3-cyanopyridine + H2O
-
648439
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648439
Rhodococcus rhodochrous
?
4-cyanopyridine + H2O
-
648439
Rhodococcus rhodochrous
pyridine-4-carbamide
-
-
-
?
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous
acetamide
-
-
-
?
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous J1
acetamide
-
-
-
?
acrylonitrile + H2O
-
648439
Rhodococcus rhodochrous
2-propenoic acid amide
i.e. acrylic acid amide
648439
Rhodococcus rhodochrous
?
benzonitrile + H2O
-
648439
Rhodococcus rhodochrous
benzoic acid amide
-
-
-
?
chloroacetonitrile + H2O
-
648439
Rhodococcus rhodochrous
chloroacetamide
-
-
-
?
chloroacetonitrile + H2O
-
648439
Rhodococcus rhodochrous J1
chloroacetamide
-
-
-
?
crotononitrile + H2O
19% of the activity with propionitrile
648439
Rhodococcus rhodochrous
(E)-2-butenoic acid amide
-
-
-
?
cyanopyrazine + H2O
-
648439
Rhodococcus rhodochrous
pyrazincarbamide
-
-
-
?
cyclopropylcyanide + H2O
-
648439
Rhodococcus rhodochrous
?
-
-
-
?
ethylene cyanhydrine + H2O
-
648439
Rhodococcus rhodochrous
?
-
-
-
?
methacrylonitrile + H2O
-
648439
Rhodococcus rhodochrous
methylacrylic acid amide
-
-
-
?
additional information
specificity overview
648439
Rhodococcus rhodochrous
?
-
-
-
-
additional information
specificity overview
648439
Rhodococcus rhodochrous J1
?
-
-
-
-
n-butyronitrile + H2O
-
648439
Rhodococcus rhodochrous
n-butyric acid amide
-
-
-
?
n-butyronitrile + H2O
-
648439
Rhodococcus rhodochrous J1
n-butyric acid amide
-
-
-
?
propionitrile + H2O
-
648439
Rhodococcus rhodochrous
propionic acid amide
-
-
-
?
propionitrile + H2O
-
648439
Rhodococcus rhodochrous J1
propionic acid amide
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Rhodococcus rhodochrous
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to, 30 min, pH 7.0, 44 mM n-butyric acid
Rhodococcus rhodochrous
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
6.8
-
Rhodococcus rhodochrous
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8
about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8
Rhodococcus rhodochrous
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
20°C, 60 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0; 25°C, 10 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0
Rhodococcus rhodochrous
General Stability (protein specific)
General Stability
Organism
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
0.01-1 mM AgNO3
Rhodococcus rhodochrous
diethyldithiocarbamate
0.1 mM
Rhodococcus rhodochrous
Hg2+
HgCl2, 0.01-1 mM
Rhodococcus rhodochrous
hydroxylamine
1 mM
Rhodococcus rhodochrous
KCN
0.01 mM
Rhodococcus rhodochrous
Na2S2O4
1 mM
Rhodococcus rhodochrous
phenylhydrazine
1 mM
Rhodococcus rhodochrous
Semicarbazide
1 mM, weak inhibition
Rhodococcus rhodochrous
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.85
-
Isobutyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
1.89
-
acrylonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
1.92
-
Propionitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.88
-
Crotononitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
6.76
-
Methacrylonitrile
pH 7.0, 20ºC
Rhodococcus rhodochrous
10
-
Cyanopyrazine
pH 7.0, 20ºC
Rhodococcus rhodochrous
12.2
-
Chloroacetonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
18.5
-
4-cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
21.7
-
2-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
21.7
-
n-butyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
28.6
-
Benzonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
200
-
3-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
contains approximately 11-12 mol cobalt/mol enzyme
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
29000
-
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
505000
530000
sedimentation equilibrium method, gel permeation HPLC
Rhodococcus rhodochrous
Purification (Commentary) (protein specific)
Commentary
Organism
; ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rhodococcus rhodochrous
371
-
purified enzyme
Rhodococcus rhodochrous
Storage Stability (protein specific)
Storage Stability
Organism
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyanopyridine + H2O
-
648439
Rhodococcus rhodochrous
pyridine-2-carbamide
-
-
-
?
3-cyanopyridine + H2O
-
648439
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648439
Rhodococcus rhodochrous
?
4-cyanopyridine + H2O
-
648439
Rhodococcus rhodochrous
pyridine-4-carbamide
-
-
-
?
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous
acetamide
-
-
-
?
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous J1
acetamide
-
-
-
?
acrylonitrile + H2O
-
648439
Rhodococcus rhodochrous
2-propenoic acid amide
i.e. acrylic acid amide
648439
Rhodococcus rhodochrous
?
benzonitrile + H2O
-
648439
Rhodococcus rhodochrous
benzoic acid amide
-
-
-
?
chloroacetonitrile + H2O
-
648439
Rhodococcus rhodochrous
chloroacetamide
-
-
-
?
chloroacetonitrile + H2O
-
648439
Rhodococcus rhodochrous J1
chloroacetamide
-
-
-
?
crotononitrile + H2O
19% of the activity with propionitrile
648439
Rhodococcus rhodochrous
(E)-2-butenoic acid amide
-
-
-
?
cyanopyrazine + H2O
-
648439
Rhodococcus rhodochrous
pyrazincarbamide
-
-
-
?
cyclopropylcyanide + H2O
-
648439
Rhodococcus rhodochrous
?
-
-
-
?
ethylene cyanhydrine + H2O
-
648439
Rhodococcus rhodochrous
?
-
-
-
?
methacrylonitrile + H2O
-
648439
Rhodococcus rhodochrous
methylacrylic acid amide
-
-
-
?
additional information
specificity overview
648439
Rhodococcus rhodochrous
?
-
-
-
-
additional information
specificity overview
648439
Rhodococcus rhodochrous J1
?
-
-
-
-
n-butyronitrile + H2O
-
648439
Rhodococcus rhodochrous
n-butyric acid amide
-
-
-
?
n-butyronitrile + H2O
-
648439
Rhodococcus rhodochrous J1
n-butyric acid amide
-
-
-
?
propionitrile + H2O
-
648439
Rhodococcus rhodochrous
propionic acid amide
-
-
-
?
propionitrile + H2O
-
648439
Rhodococcus rhodochrous J1
propionic acid amide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to, 30 min, pH 7.0, 44 mM n-butyric acid
Rhodococcus rhodochrous
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
6.8
-
Rhodococcus rhodochrous
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8
about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8
Rhodococcus rhodochrous
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
20°C, 60 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0; 25°C, 10 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0
Rhodococcus rhodochrous
Other publictions for EC 4.2.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746567
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1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
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1
-
7
-
-
3
-
-
-
4
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
3
-
-
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
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3
3
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-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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1
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
747556
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1
1
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3
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4
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-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
4
4
-
-
-
-
-
-
-
-
1
1
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
4
4
-
-
-
-
-
-
-
-
4
4
747559
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Modulation of nitrile hydrata ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
ChemCatChem
10
449-458
2018
-
-
1
-
3
-
-
8
-
-
-
-
-
4
-
-
1
-
-
-
-
-
17
-
1
-
-
8
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
8
-
-
-
-
-
-
-
1
-
-
-
-
17
-
1
-
-
8
1
-
-
-
-
-
-
-
7
7
747123
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56
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-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747192
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Monosiga brevicollis
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-
1
-
-
-
-
2
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1
1
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
749108
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-
1
1
-
-
-
2
-
-
1
2
-
-
9
-
-
1
-
-
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2
1
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1
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2
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4
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2
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2
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4
2
2
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2
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2
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-
746788
Sun
The stability enhancement of ...
Bordetella petrii, Bordetella petrii DSM 128043, Pseudonocardia thermophila
Appl. Biochem. Biotechnol.
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2016
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2
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3
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Zheng
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Rhodococcus aetherivorans, Rhodococcus aetherivorans JB1208
J. Chem. Technol. Biotechnol.
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2016
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2
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Tian
Overproduction of the Escheri ...
Rhodococcus ruber, Rhodococcus ruber TH3G
J. Microbiol. Biotechnol.
26
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2016
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749286
Sun
-
Characterization of a versati ...
Sinorhizobium meliloti, Sinorhizobium meliloti CGMCC 7333
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6
15501-15508
2016
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1
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14
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1
1
2
5
1
1
1
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1
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9
-
5
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1
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1
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14
-
1
1
2
5
1
1
1
-
-
-
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5
5
747361
Guo
-
Overexpression of a nitrile h ...
Klebsiella oxytoca, Klebsiella oxytoca DSM 4798
Biotechnol. Bioprocess Eng.
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2015
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1
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1
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19
1
1
1
4
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1
1
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1
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1
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1
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1
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1
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1
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1
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19
1
1
1
4
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1
1
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748242
Martinez
Analyzing the catalytic role ...
Comamonas testosteroni, Comamonas testosteroni Ni1
J. Biol. Inorg. Chem.
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2015
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2
1
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7
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1
5
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7
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1
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1
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2
1
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7
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728981
Rucka
Expression control of nitrile ...
Rhodococcus erythropolis, Rhodococcus erythropolis A4, Rhodococcus erythropolis CCM 2595
Antonie van Leeuwenhoek
105
1179-1190
2014
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1
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1
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4
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4
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2
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4
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4
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1
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2
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729043
Duca
Characterization of a nitrilas ...
Pseudomonas sp.
Appl. Environ. Microbiol.
80
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2014
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1
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2
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1
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2
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4
1
1
2
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1
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2
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2
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2
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2
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4
2
2
3
-
-
2
-
-
-
-
1
2
-
-
-
729148
Cui
Improvement of stability of ni ...
Comamonas testosteroni, Comamonas testosteroni 5-MGAM-4D, Pseudomonas putida, Pseudomonas putida NRRL-18668, Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
Biochem. Biophys. Res. Commun.
450
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2014
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3
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3
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3
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10
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3
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3
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5
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5
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1
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3
-
10
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
729257
Nelp
The alpha subunit of nitrile h ...
Streptomyces rimosus
Biochemistry
53
3990-3994
2014
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1
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5
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1
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3
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4
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9
1
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4
1
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3
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15
-
3
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3
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9
3
-
-
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12
3
-
-
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3
9
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4
12
729825
Hopmann
Full reaction mechanism of nit ...
Rhodococcus erythropolis
Inorg. Chem.
53
2760-2762
2014
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-
-
-
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1
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1
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1
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-
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-
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729896
Martinez
The active site sulfenic acid ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
J. Am. Chem. Soc.
136
1186-1189
2014
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1
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3
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14
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1
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4
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1
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1
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2
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2
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6
4
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2
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4
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2
-
-
-
2
-
-
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1
2
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-
730077
Liu
Enhancement of thermo-stabilit ...
Pseudomonas putida, Pseudomonas putida NRRL-18668
J. Biosci. Bioeng.
118
249-252
2014
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1
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7
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4
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1
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1
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1
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1
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1
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1
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1
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7
-
4
-
1
-
1
-
1
-
-
-
-
-
-
-
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730224
Pei
-
Discovery of a new Fe-type nit ...
Pseudomonas putida
J. Mol. Catal. B
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26-33
2014
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1
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1
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5
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1
1
2
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1
1
1
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1
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1
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1
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1
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5
-
10
-
1
1
2
-
1
1
1
-
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1
1
-
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-
746877
Kang
High-level expression in Cory ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous M33
Appl. Microbiol. Biotechnol.
98
4379-4387
2014
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1
1
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5
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1
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2
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1
1
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1
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2
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-
-
-
-
-
-
-
-
-
-
-
-
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728998
Zhang
Enzyme-substrate binding lands ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095, Rhodococcus erythropolis, Rhodococcus erythropolis AJ270
Appl. Biochem. Biotechnol.
170
1614-1623
2013
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2
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2
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4
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13
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-
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30
-
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-
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-
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-
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-
-
-
-
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2
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2
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4
-
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-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
730013
Gumataotao
Identification of an active si ...
Rhodococcus hoagii, Rhodococcus hoagii TG328-2
J. Biol. Chem.
288
15532-15536
2013
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1
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1
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1
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5
-
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1
-
-
-
-
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2
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1
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-
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1
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-
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-
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-
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1
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-
-
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-
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1
-
1
-
-
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1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
730311
Lipowicz
An aeroplysinin-1 specific nit ...
Aplysina cavernicola
Mar. Drugs
11
3046-3067
2013
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1
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-
-
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1
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4
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3
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1
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1
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2
2
1
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-
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1
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-
-
-
-
-
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1
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-
-
-
-
-
-
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1
-
4
4
-
-
-
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1
-
-
1
-
2
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
715804
Lin
Enzymatic production of 2-amin ...
Alcaligenes faecalis, Alcaligenes faecalis CCTCC M 208168, Bacillus subtilis, Bacillus subtilis CCTCC M 206038, Rhodococcus boritolerans, Rhodococcus boritolerans CCTCC M 208108, Rhodococcus ruber, Rhodococcus ruber CCTCC M 206040, Rhodococcus sp., Rhodococcus sp. N595, Serratia marcescens, Serratia marcescens CCTCC M 208231
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39
133-141
2012
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6
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-
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6
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12
-
14
-
-
-
-
-
-
-
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12
-
6
-
-
-
-
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-
-
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-
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6
-
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-
-
-
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6
-
-
-
-
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12
-
-
-
-
-
-
-
-
12
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
730090
Chen
Improving stability of nitrile ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) SC-105-1, Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095, Rhodococcus ruber, Rhodococcus ruber TH
J. Biotechnol.
164
354-362
2012
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-
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4
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17
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-
-
-
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8
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3
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
4
-
1
-
4
-
-
-
-
-
-
-
-
-
730132
Vesela
Biotransformation of benzonitr ...
Rhodococcus erythropolis, Rhodococcus erythropolis A4, Rhodococcus rhodochrous, Rhodococcus rhodochrous PA-34
J. Ind. Microbiol. Biotechnol.
39
1811-1819
2012
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2
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8
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-
-
-
-
-
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25
-
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-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
25
-
-
-
-
-
-
-
-
-
-
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-
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714254
Brodkin
Evidence of the participation ...
Pseudomonas putida, Pseudomonas putida NRRL-18668
Biochemistry
50
4923-4935
2011
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1
1
8
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12
-
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1
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6
-
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1
-
-
-
-
-
4
1
1
-
-
11
1
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
12
-
-
1
-
-
-
-
1
-
-
-
-
4
1
1
-
-
11
1
-
-
-
-
-
-
-
11
11
714580
Lin
Biosynthesis of 2-amino-2,3-di ...
Rhodococcus qingshengii, Rhodococcus qingshengii ZA0707
Biotechnol. Lett.
33
1809-1813
2011
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-
-
-
-
-
-
-
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12
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3
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-
-
-
-
-
-
12
-
1
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1
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
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12
-
-
-
-
-
-
-
-
12
-
1
-
1
-
-
-
-
-
-
-
-
-
-
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714861
Cantarella
High-yield continuous producti ...
Microbacterium imperiale, Microbacterium imperiale CBS 498-74
Enzyme Microb. Technol.
48
345-350
2011
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1
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2
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6
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4
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1
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1
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-
-
-
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1
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-
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-
-
-
-
-
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-
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2
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-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
715958
Vejvoda
-
Biotransformation of nitriles ...
Klebsiella oxytoca, Klebsiella oxytoca 38.1.2, Raoultella terrigena, Raoultella terrigena 77.1, Rhodococcus erythropolis, Rhodococcus erythropolis A4
J. Mol. Catal. B
71
51-55
2011
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3
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31
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7
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-
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37
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3
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-
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3
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-
-
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-
-
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3
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-
-
-
-
-
-
-
-
-
-
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31
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-
-
-
-
-
-
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37
-
3
-
-
-
3
-
-
-
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716057
Raczynska
Crystallographic analysis of a ...
Pyrococcus abyssi
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173
294-302
2011
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1
1
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1
1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
1
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-
-
-
-
-
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3
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1
-
-
-
1
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730543
van Pelt
Probing the enantioselectivity ...
Rhodococcus erythropolis, Rhodococcus erythropolis AJ270, Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009, Rhodopseudomonas palustris HaA2
Org. Biomol. Chem.
9
3011-3019
2011
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2
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19
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-
-
-
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16
-
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-
-
-
-
-
-
-
-
-
-
-
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-
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2
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-
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-
-
-
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16
-
-
-
-
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-
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701856
Rzeznicka
Cloning and functional express ...
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85
1417-1425
2010
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1
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1
2
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7
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1
-
-
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2
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19
1
1
-
1
-
1
-
-
1
-
-
-
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-
1
1
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1
2
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1
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2
-
19
1
1
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1
-
1
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-
702718
Ma
Identification of nitrile hydr ...
Rhodococcus ruber, Rhodococcus ruber TH
Biores. Technol.
101
285-291
2010
-
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1
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1
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5
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1
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2
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1
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1
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704017
Rose
Binding of nitric oxide to a s ...
Rhodococcus sp.
Inorg. Chem.
49
1854-1864
2010
1
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704716
Yamanaka
Kinetic and structural studies ...
Rhodococcus erythropolis, Rhodococcus erythropolis N771
J. Biol. Inorg. Chem.
15
655-665
2010
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1
1
2
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1
2
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2
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2
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705203
Chiyanzu
-
Immobilization of Geobacillus ...
Aeribacillus pallidus, Aeribacillus pallidus RAPc8
J. Mol. Catal. B
63
109-115
2010
-
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1
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1
4
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9
5
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1
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1
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4
1
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1
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9
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1
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6
-
1
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1
4
1
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706803
Black
-
Biotransformation of nitriles ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009
Tetrahedron Lett.
51
1639-1641
2010
-
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1
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2
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8
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58
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1
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1
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58
-
1
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1
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-
-
706917
Jin
-
Identification and characteriz ...
Serratia marcescens, Serratia marcescens ZJB-09104
World J. Microbiol. Biotechnol.
26
817-823
2010
-
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2
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1
-
1
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1
-
1
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714229
Zhou
Unique biogenesis of high-mole ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
Biochemistry
49
9638-9648
2010
-
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1
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1
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1
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2
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16
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1
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4
1
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1
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1
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4
-
4
1
1
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1
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1
1
-
-
-
714858
Cantarella
-
Application of continuous stir ...
Microbacterium imperiale, Microbacterium imperiale CBS 498-74
Enzyme Microb. Technol.
47
64-70
2010
-
1
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4
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1
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1
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716075
Kamble
Effect of agitation and aerati ...
Rhodococcus erythropolis, Rhodococcus erythropolis MTCC 1526
Lett. Appl. Microbiol.
51
413-420
2010
-
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5
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1
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1
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1
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1
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-
698273
O'Toole
Substrate binding preferences ...
Homo sapiens
Inorg. Chem.
48
2300-2308
2009
-
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1
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1
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701585
Van Pelt
-
Synthesis of aliphatic (S)-alp ...
Nitriliruptor alkaliphilus
Adv. Synth. Catal.
351
397-404
2009
-
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1
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1
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5
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1
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1
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-
701594
Zhang
-
Isolation and characterization ...
Rhodococcus ruber, Rhodococcus ruber CGMCC3090
Afr. J. Biotechnol.
8
5467-5475
2009
-
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2
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13
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1
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13
-
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-
703230
Sun
-
Deactivation kinetics of nitri ...
Rhodococcus sp.
Chin. J. Chem. Engin.
17
822-828
2009
-
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1
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1
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1
-
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1
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-
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-
-
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-
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-
703995
Prasad
Purification of a hyperactive ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous PA-34
Indian J. Microbiol.
49
237-242
2009
-
-
-
-
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19
1
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3
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6
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1
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1
2
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2
1
1
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1
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1
1
1
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19
-
1
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3
-
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1
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1
2
-
2
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
704478
Zhou
Self-subunit swapping chaperon ...
Rhodococcus fascians, Rhodococcus fascians DSM 43985
J. Biol. Chem.
284
14930-14938
2009
-
-
-
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1
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4
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1
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1
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1
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1
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2
-
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-
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695669
Kamble
Optimization of crucial reacti ...
Rhodococcus erythropolis, Rhodococcus erythropolis MTCC 1526
Appl. Biochem. Biotechnol.
151
143-150
2008
-
1
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4
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4
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1
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1
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1
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4
-
1
-
-
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1
-
-
-
-
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-
696768
Kubiak
Molecular dynamics simulations ...
Rhodococcus sp.
Biophys. J.
94
3824-3838
2008
1
1
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1
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1
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2
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1
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1
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1
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2
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1
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1
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1
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2
-
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-
-
-
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-
-
-
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-
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-
-
697296
Yano
-
Structural and functional mode ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
Chem. Lett.
37
672-677
2008
-
-
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1
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3
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7
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1
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4
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1
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3
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4
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
697305
Peplowski
-
Mechanical aspects of nitrile ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
Chem. Phys. Lett.
467
144-149
2008
-
1
-
1
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-
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1
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2
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7
-
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6
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1
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1
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2
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-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697734
Hopmann
-
On the role of tyrosine as cat ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095, Rhodococcus erythropolis, Rhodococcus erythropolis N-771
Eur. J. Inorg. Chem.
2008
3452-3459
2008
-
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2
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2
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4
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9
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2
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8
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2
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2
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4
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698138
van Pelt
-
Nitrile hydratase CLEAs: The i ...
Rhodococcus sp., Rhodococcus sp. J1
Green Chem.
10
395-400
2008
-
-
-
-
-
1
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2
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2
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1
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1
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1
1
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1
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-
-
-
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
698583
Sakashita
Transcriptional regulation of ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
J. Bacteriol.
190
4210-4217
2008
1
-
1
-
-
-
-
-
-
-
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2
-
15
-
-
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-
1
2
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4
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1
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1
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2
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1
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699044
Taniguchi
Novel catalytic activity of ni ...
Rhodococcus sp., Rhodococcus sp. N771
J. Biosci. Bioeng.
106
174-179
2008
-
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1
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4
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10
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1
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1
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2
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-
-
-
-
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
699597
Choi
-
Biochemical characterization o ...
Rhodococcus erythropolis, Rhodococcus erythropolis N4
J. Mol. Catal. B
55
157-163
2008
-
1
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1
14
-
-
1
5
-
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2
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-
1
-
-
-
1
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13
1
1
1
1
-
1
1
1
-
-
-
-
-
1
-
-
-
-
1
-
14
-
-
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1
5
-
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1
-
-
1
-
13
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
699611
Yu
Modeling catalytic mechanism o ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
J. Mol. Graph. Model.
27
522-528
2008
-
-
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1
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9
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1
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2
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1
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-
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699612
Liu
Insights into thermal stabilit ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) SC-105-1, Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
J. Mol. Graph. Model.
27
529-535
2008
-
-
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2
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10
-
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2
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2
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2
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2
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-
-
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2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
700890
Foerstner
A nitrile hydratase in the euk ...
Monosiga brevicollis
PLoS ONE
3
e3976
2008
-
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1
-
-
-
-
-
-
-
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5
-
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1
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1
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-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701026
Feng
-
Nitrile hydratase from Mesorhi ...
Mesorhizobium sp., Mesorhizobium sp. BNC1
Process Biochem.
43
1391-1397
2008
-
-
-
-
-
-
10
1
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1
-
6
-
3
-
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1
-
-
1
2
-
14
-
1
1
1
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1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
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1
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6
-
-
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1
-
1
2
-
14
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
671557
Kohyama
Remaining acetamide in acetoni ...
Rhodococcus pyridinivorans, Rhodococcus pyridinivorans S82-2
Appl. Microbiol. Biotechnol.
74
829-835
2007
-
1
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3
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2
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1
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2
-
-
-
-
-
-
-
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-
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-
-
-
-
671808
Song
High resolution X-ray molecula ...
Rhodococcus erythropolis, Rhodococcus erythropolis AJ270
Biochem. Biophys. Res. Commun.
362
319-324
2007
-
-
-
1
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5
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1
1
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1
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1
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1
1
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1
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-
672727
Song
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. AJ270
Biotechnol. J.
2
717-724
2007
-
-
-
-
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-
13
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4
2
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3
1
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1
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-
-
2
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24
1
1
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1
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13
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4
2
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1
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1
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-
2
-
24
1
1
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-
-
-
1
-
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-
-
-
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-
-
672758
Song
Efficient expression in E. col ...
Rhodococcus erythropolis, Rhodococcus erythropolis AJ270
Biotechnol. Lett.
30
755-762
2007
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-
1
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7
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1
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4
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1
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1
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4
-
2
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-
673098
Wang
-
Inducing expression and reacti ...
Rhodococcus sp., Rhodococcus sp. SHZ-1
Chin. J. Chem. Engin.
15
573-578
2007
2
-
-
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2
1
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-
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2
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8
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2
-
2
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1
-
1
1
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1
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2
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2
1
1
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8
-
2
-
2
-
1
-
1
1
-
-
-
-
-
-
-
-
673399
Yeom
-
Enantioselective production of ...
Comamonas oleophilus, Nocardia transvalensis, Rhodococcus erythropolis, Rhodococcus rhodochrous
Enzyme Microb. Technol.
41
842-848
2007
-
1
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-
-
-
-
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4
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4
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4
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1
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1
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1
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4
-
4
-
1
-
-
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1
-
-
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-
-
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674012
Agerbirk
Host plant-dependent metabolis ...
no activity in Arabidopsis thaliana, Sinapis alba, Sinapis arvensis
Insect Biochem. Mol. Biol.
37
1119-1130
2007
-
-
-
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2
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8
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2
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2
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2
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2
-
2
-
-
-
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675242
Sari
Influence of cobalt substituti ...
Comamonas testosteroni
J. Inorg. Biochem.
101
614-622
2007
-
-
1
-
1
-
11
2
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-
3
-
-
3
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1
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5
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1
1
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1
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7
-
16
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1
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-
1
-
16
11
7
2
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-
3
-
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1
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-
5
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
675502
Peplowski
Insights into catalytic activi ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
J. Mol. Model.
13
725-730
2007
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-
-
1
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8
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1
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8
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1
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8
-
-
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-
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-
-
-
-
676087
Okamoto
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. RHA1
Mol. Microbiol.
65
828-838
2007
-
1
-
-
-
-
6
4
-
5
3
-
-
6
-
-
1
-
-
-
4
-
10
2
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-
4
1
1
-
-
-
-
-
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1
-
-
-
-
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6
-
4
-
5
3
-
-
-
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1
-
-
4
-
10
2
-
-
-
4
1
1
-
-
-
-
-
-
-
-
677265
Wang
-
Characterization of nitrile hy ...
Nocardia sp., Nocardia sp. 108
World J. Microbiol. Biotechnol.
23
355-362
2007
-
1
-
-
-
-
-
-
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1
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3
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-
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13
-
1
1
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2
2
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1
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1
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-
-
-
13
-
1
1
-
-
2
2
-
-
-
-
-
-
-
-
663744
Kohyama
Convenient treatment of aceton ...
Brevundimonas diminuta, Rhodococcus pyridinivorans, Rhodococcus pyridinivorans S85-2
Appl. Microbiol. Biotechnol.
72
600-606
2006
-
2
-
-
-
-
-
-
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2
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8
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1
2
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2
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2
2
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2
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2
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-
1
2
-
2
-
2
2
-
-
-
-
-
-
-
-
663896
Tastan Bishop
A new approach to possible sub ...
Pseudonocardia thermophila
Biochem. Biophys. Res. Commun.
343
319-325
2006
-
-
-
1
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2
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1
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1
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-
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-
-
-
-
-
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-
-
-
-
671402
Wang
-
Stability study on the nitrile ...
Nocardia sp., Nocardia sp. 108
Appl. Biochem. Microbiol.
42
384-387
2006
-
-
-
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1
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3
-
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1
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-
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1
2
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1
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1
1
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-
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-
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1
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1
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-
-
1
2
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
672689
Takarada
Mutational study on alphaGln90 ...
Rhodococcus sp., Rhodococcus sp. N771
Biosci. Biotechnol. Biochem.
70
881-889
2006
-
-
-
1
3
1
1
4
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
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4
-
-
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-
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1
3
1
-
1
4
4
-
-
-
-
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1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
675472
Yu
-
An over expression and high ef ...
Nocardia sp., Nocardia sp. YS-2002
J. Mol. Catal. B
43
80-85
2006
-
-
1
-
4
-
1
2
-
-
-
-
-
4
-
-
-
-
-
-
7
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
2
-
-
-
-
-
-
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-
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-
7
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
663536
Hourai
Cloning, purification, crystal ...
Bacillus smithii, Bacillus smithii SC-J05-1
Acta Crystallogr. Sect. F
61
974-977
2005
-
-
-
1
-
-
-
-
-
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3
-
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4
-
-
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-
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1
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-
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1
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3
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663731
Petrillo
Over-expression in Escherichia ...
Comamonas testosteroni, Comamonas testosteroni 5-MGAM-4D
Appl. Microbiol. Biotechnol.
67
664-670
2005
-
-
1
-
-
-
-
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2
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5
-
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-
-
-
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13
1
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-
-
-
-
-
-
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-
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-
1
-
-
-
-
-
-
-
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-
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2
-
-
-
-
-
-
-
-
-
13
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648457
Miyanaga
Mutational and structural anal ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
Eur. J. Biochem.
271
429-438
2004
1
-
1
1
5
-
3
15
-
2
-
-
-
22
-
-
1
-
-
-
-
2
20
-
1
-
-
14
1
-
-
-
4
-
-
2
-
2
-
2
8
-
-
7
8
30
-
4
-
-
-
-
-
2
-
-
-
2
20
-
2
-
-
28
2
-
-
-
-
1
2
-
14
28
654314
Brady
Characterisation of nitrilase ...
Pseudomonas fluorescens, Rhodococcus sp., Rhodococcus sp. Novo SP361
Appl. Microbiol. Biotechnol.
64
76-85
2004
-
1
-
-
-
-
-
-
-
-
-
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4
-
-
-
-
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
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-
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-
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664408
Precigou
Rhodococcus pyridinovorans MW3 ...
Rhodococcus pyridinivorans, Rhodococcus pyridinivorans MW3
Biotechnol. Lett.
26
1379-1384
2004
1
-
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2
-
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1
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7
-
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-
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4
-
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-
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-
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2
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-
-
2
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-
-
2
-
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-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664412
Reisinger
Enzymatic hydrolysis of cyanoh ...
Rhodococcus erythropolis
Biotechnol. Lett.
26
1675-1680
2004
-
-
-
-
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-
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3
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4
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-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665421
Suzuki
Thermal equilibrium of two con ...
Rhodococcus sp.
J. Biochem.
136
115-121
2004
1
-
-
-
-
-
1
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1
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2
-
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1
-
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666088
Cantarella
-
A study in UF-membrane reactor ...
Microbacterium imperiale, Microbacterium imperiale CBS 498-74
J. Mol. Catal. B
29
105-113
2004
-
1
-
-
-
-
-
1
-
-
-
-
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5
-
-
-
-
-
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1
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2
-
-
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4
-
-
-
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-
-
1
-
-
-
-
-
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-
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1
-
-
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-
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-
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-
-
-
1
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
666090
Kashiwagi
-
Control of the nitrile-hydroly ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous IFO 15564
J. Mol. Catal. B
29
249-258
2004
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
11
-
1
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
11
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
666211
Brandao
-
Bioconversion of D,L-tert-leuc ...
Rhodococcus erythropolis, Rhodococcus erythropolis 870-AN019
Life Sci.
4
547-556
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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1
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-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
666930
Tsekoa
-
Purification, crystallization ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) RAPc8
South Afr. J. Sci.
100
488-490
2004
-
-
-
1
-
-
-
-
-
-
-
-
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2
-
-
1
-
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-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
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-
-
-
-
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-
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-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648443
Stevens
Chaperone-assisted expression, ...
Comamonas testosteroni
Protein Expr. Purif.
29
70-76
2003
-
-
1
-
-
-
-
4
-
1
3
-
-
3
-
-
1
-
-
-
4
1
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
3
-
-
-
-
1
-
-
4
1
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729123
Miyanaga
Crystal structure of cobalt-co ...
Pseudonocardia thermophila, Pseudonocardia thermophila JCM 3095
Biochem. Biophys. Res. Commun.
288
1169-1174
2001
-
-
1
1
-
-
1
-
-
3
-
-
-
14
-
-
1
-
-
-
1
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
6
-
-
-
-
-
2
-
-
2
-
4
2
2
-
-
-
-
-
-
-
-
1
2
-
-
-
648442
Murakami
Post-translational modificatio ...
Rhodococcus sp.
Protein Sci.
9
1024-1030
2000
-
1
1
-
-
-
-
-
-
1
1
-
-
2
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648456
Tauber
Nitrile hydratase and amidase ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 11216
Appl. Environ. Microbiol.
66
1634-1638
2000
1
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648445
Kaufmann
Nitrile hydratase from Rhodoco ...
Rhodococcus erythropolis, Rhodococcus rhodochrous
Steroids
64
535-540
1999
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648446
Nojiri
Functional expression of nitri ...
Rhodococcus sp.
J. Biochem.
125
696-704
1999
2
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1
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648449
Nakasako
Tertiary and quaternary struct ...
Rhodococcus sp.
Biochemistry
38
9887-9898
1999
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658870
Petersen
-
Biocatalysis - Preparation and ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
Green Chem.
4
99-106
1999
-
1
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14
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2
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648448
Wieser
-
Low-molecular-mass nitrile hyd ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
FEMS Microbiol. Lett.
169
17-22
1998
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1
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3
24
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1
4
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14
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1
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16
1
1
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1
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1
1
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-
648453
Pereira
A novel thermostable nitrile h ...
Bacillus sp. (in: Bacteria)
Extremophiles
2
347-357
1998
-
-
-
-
-
-
-
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1
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1
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1
1
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1
2
-
1
-
-
-
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-
-
-
-
648454
Kobayashi
Metalloenzyme nitrile hydratas ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23, Pseudomonas putida, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus sp., Rhodococcus sp. N-774, Rhodococcus sp. R312
Nat. Biotechnol.
16
733-736
1998
1
-
1
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2
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36
-
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11
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11
2
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648451
Payne
A stereoselective cobalt-conta ...
Pseudomonas putida, Pseudomonas putida NRRL-18668
Biochemistry
36
5447-5454
1997
-
-
1
-
-
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-
1
-
1
4
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7
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1
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2
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4
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1
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1
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4
2
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1
-
1
-
-
-
-
-
-
-
-
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648452
Wu
Over-production of stereoselec ...
Pseudomonas putida 5B, Pseudomonas putida
Appl. Microbiol. Biotechnol.
48
704-708
1997
1
-
1
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1
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5
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6
1
-
-
-
-
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-
-
-
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-
-
-
-
648444
Yamada
Nitrile hydratase and its appl ...
Agrobacterium tumefaciens, Albifimbria verrucaria, Arthrobacter sp., Pseudomonas chlororaphis, Pseudomonas chlororaphis B23, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus sp.
Biosci. Biotechnol. Biochem.
60
1391-1400
1996
4
-
3
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-
1
1
3
-
6
3
2
-
31
-
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-
-
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-
3
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36
2
1
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1
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1
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1
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4
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3
1
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1
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1
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6
3
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3
-
36
2
1
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1
-
1
-
-
-
-
-
-
-
-
-
648447
Kopf
Key role of alkanoic acids on ...
Brevibacterium sp., Brevibacterium sp. R312
Eur. J. Biochem.
240
239-244
1996
-
-
-
-
-
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5
1
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1
1
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13
-
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1
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2
1
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5
1
1
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1
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1
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2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648450
Kobayashi
-
Hyperinduction of nitrile hydr ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens B-261
Appl. Microbiol. Biotechnol.
45
176-181
1996
-
-
-
-
-
-
2
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2
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1
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7
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1
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1
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2
-
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-
-
-
-
-
-
1
-
7
-
1
-
1
-
-
-
-
-
-
-
718334
Kobayashi
Occurrence of enzymes involved ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens IAM B-261, Mesorhizobium loti 02-10055, Mesorhizobium loti 02-10056, Mesorhizobium loti 02-10101, Mesorhizobium loti, Rhizobium leguminosarum 02-03119, Rhizobium leguminosarum 02-10041, Rhizobium leguminosarum 02-10230, Rhizobium leguminosarum, Sinorhizobium meliloti 03-03046, Sinorhizobium meliloti
Proc. Natl. Acad. Sci. USA
92
714-718
1995
-
-
-
-
-
-
-
2
-
3
2
12
-
16
-
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1
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12
-
18
1
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4
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2
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3
2
12
-
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1
-
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12
-
18
1
-
-
-
-
4
-
-
-
3
-
-
3
-
-
648435
Moreau
-
Purification and characterizat ...
Brevibacterium sp., Brevibacterium sp. R312
J. Basic Microbiol.
33
323-329
1993
-
-
-
-
-
-
16
9
-
-
3
-
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12
-
-
1
-
-
-
1
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10
1
1
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1
-
1
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13
-
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-
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-
-
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16
13
9
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3
-
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1
-
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1
-
10
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
648429
Li
-
Formation and purification of ...
Corynebacterium pseudodiphtheriticum, Corynebacterium pseudodiphtheriticum ZBB-41
Appl. Biochem. Biotechnol.
36
171-181
1992
-
1
-
-
-
-
8
2
-
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3
-
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2
-
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1
-
-
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2
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2
1
1
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2
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1
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1
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1
1
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1
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8
1
2
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3
-
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1
-
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2
-
2
1
1
-
2
-
1
-
1
1
-
-
-
-
-
-
648436
Duran
The N-terminal amino acid sequ ...
Arthrobacter sp., Brevibacterium sp., Brevibacterium sp. R312, Corynebacterium sp., Pseudomonas chlororaphis, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus sp., Rhodococcus sp. 7, Rhodococcus sp. N-774
J. Basic Microbiol.
32
13-19
1992
-
-
-
-
-
-
-
-
-
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18
-
-
39
-
-
1
-
-
-
6
-
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7
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1
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-
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-
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18
-
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1
-
-
6
-
-
7
-
-
-
-
-
-
-
1
-
-
-
-
-
-
648428
Kobayashi
Cloning, nucleotide sequence a ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus sp., Rhodococcus sp. N-774
Biochim. Biophys. Acta
1129
23-33
1991
-
-
1
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-
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-
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4
-
-
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24
-
-
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-
-
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1
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4
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648430
Nagamune
Crystallization of a photosens ...
Rhodococcus sp., Rhodococcus sp. N-774
J. Mol. Biol.
220
221-222
1991
1
-
-
1
-
-
-
-
-
-
-
-
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7
-
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1
1
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-
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648431
Nelson
-
A novel iron-sulfur center in ...
Brevibacterium sp., Brevibacterium sp. R312
J. Am. Chem. Soc.
113
7072-7073
1991
-
-
-
-
-
-
-
-
-
1
1
-
-
12
-
-
-
-
-
-
-
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1
-
-
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-
-
-
-
-
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-
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-
-
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1
1
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-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648434
Kaakeh
-
Purification and properties of ...
Rhodococcus sp., Rhodococcus sp. 7
Zentralbl. Mikrobiol.
146
89-98
1991
-
-
-
1
-
-
6
6
1
-
3
-
-
3
-
-
1
-
-
-
4
-
11
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
6
1
-
3
-
-
-
-
1
-
-
4
-
11
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
648439
Nagasawa
Characterization of a new coba ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
Eur. J. Biochem.
196
581-589
1991
-
-
-
-
-
1
8
12
-
1
3
-
-
16
-
-
1
-
-
-
2
1
20
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
8
-
12
-
1
3
-
-
-
-
1
-
-
2
1
20
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
648425
Hjort
-
Isolation and characterization ...
Rhodococcus sp.
J. Chem. Technol. Biotechnol.
48
217-226
1990
-
1
-
-
-
-
8
3
-
-
3
-
-
1
-
-
1
-
-
-
1
-
6
1
-
-
2
-
1
1
1
-
2
-
-
-
1
-
-
-
-
-
-
8
2
3
-
-
3
-
-
-
-
1
-
-
1
-
6
1
-
-
2
-
1
1
1
-
-
-
-
-
-
-
648423
Amarant
-
Substrates and inhibitors of t ...
Corynebacterium nitrilophilus
Biotechnol. Appl. Biochem.
11
49-59
1989
1
-
-
-
-
-
5
-
1
-
-
-
-
1
-
-
1
-
-
-
-
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1
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-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
648437
Tani
-
Characterization of nitrile hy ...
Corynebacterium sp., Corynebacterium sp. C5
Agric. Biol. Chem.
53
3151-3158
1989
-
-
-
-
-
1
13
1
-
1
2
-
-
3
-
-
1
-
-
-
1
-
21
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
13
-
1
-
1
2
-
-
-
-
1
-
-
1
-
21
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
648441
Endo
Nitrile hydratase of Rhodococc ...
Rhodococcus sp., Rhodococcus sp. N-774
FEBS Lett.
243
61-64
1989
-
1
-
1
-
-
-
-
-
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3
-
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7
-
-
1
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
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1
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1
-
-
-
-
-
-
-
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3
-
-
-
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1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648433
Nagasawa
Occurrence of a cobalt-induced ...
Pseudomonas chlororaphis, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
Biochem. Biophys. Res. Commun.
155
1008-1016
1988
-
2
-
-
-
-
-
-
-
3
3
-
-
19
-
-
1
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
-
-
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3
3
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1
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648424
Nakajima
-
Photoactivation of nitrile hyd ...
Corynebacterium sp., Corynebacterium sp. N-774
Chem. Lett.
9
1767-1770
1987
-
-
-
-
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1
-
-
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2
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648427
Sugiura
-
Nitrile hydratase: The first n ...
Brevibacterium sp., Brevibacterium sp. R312, Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
J. Am. Chem. Soc.
109
5848-5850
1987
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-
-
2
-
-
-
-
-
2
3
-
-
25
-
-
-
-
-
-
-
-
4
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
4
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648438
Nagasawa
Nitrile hydratase is a quinopr ...
Brevibacterium sp., Brevibacterium sp. R312
Biochem. Biophys. Res. Commun.
147
701-709
1987
-
1
-
-
-
-
5
-
-
1
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648440
Nagasawa
Nitrile hydratase of Pseudomon ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
Eur. J. Biochem.
162
691-698
1987
-
1
-
1
-
1
18
6
-
1
2
-
-
15
-
-
1
-
-
-
2
1
16
1
1
2
6
1
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
18
-
6
-
1
2
-
-
-
-
1
-
-
2
1
16
1
1
2
6
1
1
-
-
-
-
-
-
-
-
-
648432
Nagasawa
Nitrile hydratase of Brevibact ...
Brevibacterium sp., Brevibacterium sp. R312
Biochem. Biophys. Res. Commun.
139
1305-1312
1986
-
-
-
1
-
-
1
7
-
1
6
-
-
13
-
-
1
-
-
-
2
-
21
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
1
7
-
1
6
-
-
-
-
1
-
-
2
-
21
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
657500
Yamada
-
Optimum culture conditions for ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
Agric. Biol. Chem.
50
2859-2865
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
648426
Bui
-
A nitrile hydratase with a wid ...
Brevibacterium sp., Brevibacterium sp. R312
J. Gen. Microbiol.
130
89-93
1984
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-
-
-
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-
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2
-
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12
-
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1
-
-
-
-
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19
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
19
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
648422
Asano
-
Aliphatic nitrile hydratase fr ...
Arthrobacter sp., Arthrobacter sp. J-1
Agric. Biol. Chem.
46
1165-1174
1982
1
-
-
-
-
1
9
6
1
-
3
-
-
3
-
-
1
-
-
-
1
2
13
1
1
-
2
-
1
-
-
-
1
1
-
1
-
-
-
-
-
1
-
9
1
6
1
-
3
-
-
-
-
1
-
-
1
2
13
1
1
-
2
-
1
-
-
1
-
-
-
-
-
-