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Literature summary for 4.2.1.84 extracted from

  • Nagasawa, T.; Takeuchi, K.; Yamada, H.
    Occurrence of a cobalt-induced and cobalt-containing nitrile hydratase in Rhodococcus rhodochrous J1 (1988), Biochem. Biophys. Res. Commun., 155, 1008-1016.
    View publication on PubMed
Search for more literature for 4.2.1.84

Application

Application Comment Organism
synthesis
-
 Rhodococcus rhodochrous
synthesis
-
 Pseudomonas chlororaphis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5.7 atoms cobalt/mol enzyme, tightly bound to the protein Rhodococcus rhodochrous
Co2+ indispensable for high enzyme activity Rhodococcus rhodochrous
Co2+ 0.1% w/v, greatly increases the enzyme activity. Different Co2+ compounds increase the enzyme activity Rhodococcus rhodochrous
Co2+ enzyme of J1 strain contains cobalt Rhodococcus rhodochrous
Fe2+ at 0.1% w/v does not enhance the enzyme activity Rhodococcus rhodochrous
additional information CuSO4, CaCl2, ZnSO4, MnCl2, NaMnO4, FeCl3, FeSO4, AlCl3, BaCl2, SrCl2, NaWO4, SnCl2, BeSO4, NiCl2, PbCl2, LiCl and CoCl2 at 0.1% w/v neither inhibit nor enhance the enzyme activity Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
29000
-
 x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
505000 530000 sedimentation equilibrium method, gel permeation HPLC Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous J1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus rhodochrous
ammonium sulfate fractionation, chromatography on DEAE-Sephacel, octyl-Sepharose CL-4B and phenyl-Sepharose CL-4B, 4.91fold purification Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodococcus rhodochrous
additional information
-
-
 Pseudomonas chlororaphis
0.084
-
-
 Rhodococcus rhodochrous
5.21
-
 with the addition of 0.1% w/v CoCl2 Rhodococcus rhodochrous

Subunits

Subunits Comment Organism
? x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous