BRENDA - Enzyme Database show
show all sequences of 4.2.1.65

Microbial utilization of beta-cyano-L-alanine by Pseudomonas sp. 13

Yanase, H.; Sakai, T.; Tonomura, K.; Agric. Biol. Chem. 46, 2925-2932 (1982)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
not stabilized by rapid freezing in liquid nitrogen, storage at 4 or -20¦C with mercaptoethanol, dithiothreitol, beta-cyano-L-alanine or asparagine
Pseudomonas sp.
stabilized as ammonium sulfate precipitate
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
13
-
Pseudomonas sp. 13
-
13
-
Storage Stability
Storage Stability
Organism
-20°C, 10% glycerol, 10-15% loss of activity after 5 days
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5825
Pseudomonas sp.
L-Asparagine
asparagine is subsequently degraded to aspartic acid and ammonia
5825
Pseudomonas sp.
-
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5825
Pseudomonas sp. 13
L-Asparagine
asparagine is subsequently degraded to aspartic acid and ammonia
5825
Pseudomonas sp. 13
-
General Stability (protein specific)
General Stability
Organism
not stabilized by rapid freezing in liquid nitrogen, storage at 4 or -20¦C with mercaptoethanol, dithiothreitol, beta-cyano-L-alanine or asparagine
Pseudomonas sp.
stabilized as ammonium sulfate precipitate
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10% glycerol, 10-15% loss of activity after 5 days
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5825
Pseudomonas sp.
L-Asparagine
asparagine is subsequently degraded to aspartic acid and ammonia
5825
Pseudomonas sp.
-
beta-Cyano-L-alanine + H2O
highly specific for beta-cyano-L-alanine
5825
Pseudomonas sp. 13
L-Asparagine
asparagine is subsequently degraded to aspartic acid and ammonia
5825
Pseudomonas sp. 13
-
Other publictions for EC 4.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670571
Piotrowski
Cyanide metabolism in higher p ...
Lupinus angustifolius
Plant Mol. Biol.
61
111-122
2006
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1
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5824
Yanase
-
Metabolism of nitriles in micr ...
Pseudomonas sp., Pseudomonas sp. 13
Agric. Biol. Chem.
47
473-482
1983
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1
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1
12
1
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2
2
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3
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4
1
1
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1
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1
1
1
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1
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1
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12
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1
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2
2
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1
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1
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4
1
1
1
1
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1
1
1
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5825
Yanase
-
Microbial utilization of beta- ...
Pseudomonas sp., Pseudomonas sp. 13
Agric. Biol. Chem.
46
2925-2932
1982
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2
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5826
Castric
Cyanide metabolism in higher p ...
Lupinus angustifolius, Sorghum bicolor
Arch. Biochem. Biophys.
152
62-69
1972
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6
1
1
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1
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2
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1
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4
2
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2
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1
1
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6
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1
1
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1
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1
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4
2
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2
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1
1
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5827
Castric
Cyanide metabolism by Bacillus ...
Bacillus megaterium
J. Biol. Chem.
244
4089-4094
1969
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