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Information on EC 4.2.1.65 - 3-Cyanoalanine hydratase
for references in articles please use BRENDA:EC4.2.1.65
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
showSynonyms
beta-cyanoalanine hydratase, beta-cyanoalanine hydrolase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Beta-CNA nitrilase
-
-
-
-
Beta-CNAla hydrolase
-
-
-
-
beta-Cyanoalanine hydratase
-
-
-
-
beta-Cyanoalanine hydrolase
-
-
-
-
Hydratase, beta-cyanoalanine
-
-
-
-
Hydrolase, beta-cyanoalanine
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-Asparagine = 3-cyanoalanine + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
addition
-
-
-
-
hydrolysis
-
-
of nitrile group
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
cyanide detoxification I
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SYSTEMATIC NAME
IUBMB Comments
L-asparagine hydro-lyase (3-cyanoalanine-forming)
-
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CAS REGISTRY NUMBER
COMMENTARY
37259-64-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-Cyano-L-alanine + H2O
?
-
Substrates: cyanide assimilation
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
?
-
Substrates: cyanide assimilation
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
L-Asparagine
Substrates: -
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
L-Asparagine
Substrates: enzyme involved in the cyanide detoxification pathway
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
L-Asparagine
-
Substrates: highly specific for beta-cyano-L-alanine
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
L-Asparagine
-
Substrates: highly specific for beta-cyano-L-alanine
Products: asparagine is subsequently degraded to aspartic acid and ammonia
Products: asparagine is subsequently degraded to aspartic acid and ammonia
?
beta-Cyano-L-alanine + H2O
L-Asparagine
-
Substrates: highly specific for beta-cyano-L-alanine
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
L-Asparagine
-
Substrates: highly specific for beta-cyano-L-alanine
Products: asparagine is subsequently degraded to aspartic acid and ammonia
Products: asparagine is subsequently degraded to aspartic acid and ammonia
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-Cyano-L-alanine + H2O
L-Asparagine
Substrates: enzyme involved in the cyanide detoxification pathway
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
?
-
Substrates: cyanide assimilation
Products: -
Products: -
?
beta-Cyano-L-alanine + H2O
?
-
Substrates: cyanide assimilation
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ag+
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Cd2+
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Cu2+
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
HgCl2
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.371
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NRL4A_LUPAN
349
0
37762
Swiss-Prot
other Location (Reliability: 3)
NRL4A_TOBAC
349
0
37555
Swiss-Prot
other Location (Reliability: 3)
NRL4B_LUPAN
350
0
38055
Swiss-Prot
other Location (Reliability: 2)
NRL4_ARATH
355
0
38895
Swiss-Prot
other Location (Reliability: 3)
A0A2P6QEB8_ROSCH
351
0
37690
TrEMBL
other Location (Reliability: 4)
A0A396IC97_MEDTR
125
0
13487
TrEMBL
other Location (Reliability: 5)
A0A396I498_MEDTR
205
0
22701
TrEMBL
other Location (Reliability: 2)
A0A072UKA3_MEDTR
350
0
37868
TrEMBL
other Location (Reliability: 2)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
not stabilized by rapid freezing in liquid nitrogen, storage at 4 or -20¦C with mercaptoethanol, dithiothreitol, beta-cyano-L-alanine or asparagine
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yanase, H.; Sakai, T.; Tonomura, K.
Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13
Agric. Biol. Chem.
47
473-482
1983
Pseudomonas sp., Pseudomonas sp. 13
-
brenda
Yanase, H.; Sakai, T.; Tonomura, K.
Microbial utilization of beta-cyano-L-alanine by Pseudomonas sp. 13
Agric. Biol. Chem.
46
2925-2932
1982
Pseudomonas sp., Pseudomonas sp. 13
-
brenda
Castric, P.A.; Farnden, J.F.; Conn, E.E.
Cyanide metabolism in higher plants. V. The formation of asparagine from beta-cyanoalanine
Arch. Biochem. Biophys.
152
62-69
1972
Lupinus angustifolius, Sorghum bicolor
brenda
Castric, P.A.; Strovel, G.A.
Cyanide metabolism by Bacillus megaterium
J. Biol. Chem.
244
4089-4094
1969
Priestia megaterium
brenda
Piotrowski, M.; Volmer, J.J.
Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog
Plant Mol. Biol.
61
111-122
2006
Lupinus angustifolius (Q5QGZ8)
brenda
EXTERNAL LINKS (specific for EC-Number 4.2.1.65)
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Release 2023.1 (January 2023)
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