Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as glutathione S-transferase fusion protein | Paramecium bursaria Chlorella virus-1 |
expressed in Escherichia coli as glutathione S-transferase fusion protein | Acanthocystis turfacea chlorella virus 1 |
expression in Escherichia coli | Paramecium bursaria Chlorella virus-1 |
expression in Escherichia coli | Acanthocystis turfacea chlorella virus 1 |
General Stability | Organism |
---|---|
NADPH is essential for stabilization and function of the enzyme | Paramecium bursaria Chlorella virus-1 |
NADPH is essential for stabilization and function of the enzyme | Acanthocystis turfacea chlorella virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | conversion of NADPH to NADP+ results in complete inactivation of the enzyme | Acanthocystis turfacea chlorella virus 1 | |
NADP+ | conversion of NADPH to NADP+ results in complete inactivation of the enzyme | Paramecium bursaria Chlorella virus-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
gel filtration | Paramecium bursaria Chlorella virus-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthocystis turfacea chlorella virus 1 | - |
- |
- |
Paramecium bursaria Chlorella virus-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paramecium bursaria Chlorella virus-1 |
- |
Acanthocystis turfacea chlorella virus 1 |
GSH-Sepharose 4B column chromatography | Paramecium bursaria Chlorella virus-1 |
GSH-Sepharose 4B column chromatography | Acanthocystis turfacea chlorella virus 1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.48 | - |
- |
Paramecium bursaria Chlorella virus-1 |
0.63 | - |
- |
Acanthocystis turfacea chlorella virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-D-mannose | - |
Paramecium bursaria Chlorella virus-1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
GDP-D-mannose | - |
Acanthocystis turfacea chlorella virus 1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
GDP-mannose | ATCV-1GMD has no reductase activity | Paramecium bursaria Chlorella virus-1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
GDP-mannose | ATCV-1GMD has no reductase activity | Acanthocystis turfacea chlorella virus 1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | NADPH is essential for maintaining the oligomerization status | Paramecium bursaria Chlorella virus-1 |
More | NADPH is essential for maintaining the oligomerization status | Acanthocystis turfacea chlorella virus 1 |
Synonyms | Comment | Organism |
---|---|---|
ATCV-1GMD | - |
Acanthocystis turfacea chlorella virus 1 |
GDP-D-mannose 4,6-dehydratase | - |
Paramecium bursaria Chlorella virus-1 |
GDP-D-mannose 4,6-dehydratase | - |
Acanthocystis turfacea chlorella virus 1 |
GMD | - |
Paramecium bursaria Chlorella virus-1 |
GMD | - |
Acanthocystis turfacea chlorella virus 1 |
PBCV-1 GMD | - |
Paramecium bursaria Chlorella virus-1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme has a very low affinity for NADP+ | Paramecium bursaria Chlorella virus-1 | |
additional information | the enzyme has a very low affinity for NADP+ | Acanthocystis turfacea chlorella virus 1 | |
NADPH | dependent on | Paramecium bursaria Chlorella virus-1 | |
NADPH | dependent on | Acanthocystis turfacea chlorella virus 1 | |
NADPH | the enzymatically active proteins contain tightly bound NADPH that essential for maintaining the oligomerization status as well as for the stabilization and function of the enzyme | Paramecium bursaria Chlorella virus-1 | |
NADPH | the enzymatically active proteins contain tightly bound NADPH that is essential for maintaining the oligomerization status as well as for the stabilization and function of the enzyme | Acanthocystis turfacea chlorella virus 1 |