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Literature summary for 4.2.1.39 extracted from

  • Matsubara, K.; Koehling, R.; Schoenenberger, B.; Kouril, T.; Esser, D.; Braesen, C.; Siebers, B.; Wohlgemuth, R.
    One-step synthesis of 2-keto-3-deoxy-D-gluconate by biocatalytic dehydration of D-gluconate (2014), J. Biotechnol., 191, 69-77 .
    View publication on PubMed

Application

Application Comment Organism
synthesis one-step biocatalytic synthesis procedure for 2-keto-3-deoxy-D-gluconate from D-gluconate using recombinant gluconate dehydratase. The D-gluconate is completely converted to stereochemically pure D-2-keto-3-deoxy-D-gluconate without side-product formation, the final 2-keto-3-deoxy-D-gluconate yield is approximately 90%, the quantitative and qualitative LC-MS analysis method enables the simultaneous detection of D-gluconate and 2-keto-3-deoxy-D-gluconate and the enzyme can be provided by a simple and rapid procedure involving only two precipitation steps Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax G4RJQ1
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Thermoproteus tenax DSM 2078 G4RJQ1
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Purification (Commentary)

Purification (Comment) Organism
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Thermoproteus tenax

Synonyms

Synonyms Comment Organism
TTX_1156
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Thermoproteus tenax