Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl compounds | Fe2+ and sulfhydryl compound required, cysteine, Na-thioglycollate, and glutathione equally effective | Clostridium aceticum |
Application | Comment | Organism |
---|---|---|
synthesis | utilization for fermentation 2-keto-3-deoxy-D-gluconate proposed, conditions for fermentation | Clostridium aceticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace | Clostridium aceticum | |
Fe2+ | Fe2+ and sulfhydryl compound required | Clostridium aceticum | |
Mg2+ | Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace | Clostridium aceticum | |
Mn2+ | Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace | Clostridium aceticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | Clostridium aceticum | first enzyme in modified Entner-Doudoroff pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium aceticum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
cell-free extract, fructose grown | Clostridium aceticum |
10 | - |
cell-free extract, gluconate grown | Clostridium aceticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | first enzyme in modified Entner-Doudoroff pathway | Clostridium aceticum | ? | - |
? | |
D-mannonate | 16% of activity with D-gluconate | Clostridium aceticum | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
enzyme assay at | Clostridium aceticum |