Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidaminococcus fermentans | P11569 and P11570 and P11568 | P11569 i.e. subunit alpha, P11570 i.e. subunit beta, P11568 i.e. activator protein | - |
Acidaminococcus fermentans DSM 20731 | P11569 and P11570 and P11568 | P11569 i.e. subunit alpha, P11570 i.e. subunit beta, P11568 i.e. activator protein | - |
Storage Stability | Organism |
---|---|
homodimeric activator, in the presence of 5 mM MgCl2 and 1 mM ADP or ATP, can be stabilized and stored for 4 days at 4°C without loss of activity | Acidaminococcus fermentans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
riboflavin | presence of trace amounts | Acidaminococcus fermentans | |
riboflavin 5'-phosphate | 1 mol per mol of heterodimeric dehydratase | Acidaminococcus fermentans | |
[4Fe-4S]-center | each active component contains an oxygen sensitive diamagnetic [4Fe-4S]2+ cluster. Reduction of the [4Fe-4S]2+ cluster of the activator protein with dithionite yields a paramagnetic [4Fe-4S]1+ cluster with the unusual electron spin ground state S=3/2. Under air the activator protein looses its activity within seconds due to irreversible degradation of its [4Fe-4S]2+ cluster to a [2Fe-2S]2+ cluster. The [4Fe-4S]2+ cluster of the heterodimeric dehydratase cannot be reduced to a [4Fe-4S]1+ cluster | Acidaminococcus fermentans |