Cloned (Comment) | Organism |
---|---|
wild type (HsMFE-2) and its variants are expressed in Saccharomyces cerevisiae, the recombinant HsMFE-2(dhdelta) and its variants are expressed in Escherichia coli BL21(DE3)pLysS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D370A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
D490A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
D510A | inactive mutant enzyme | Homo sapiens |
D517A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
E366A | kcat/Km 100times lower than that of the wild type | Homo sapiens |
E408A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
G16S | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
H406A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
H515A | inactive mutant enzyme | Homo sapiens |
H532A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
additional information | mutant constructs are tested for complementation in Saccharomyces cerevisiae | Homo sapiens |
Y347A | inactive mutant enzyme | Homo sapiens |
Y410A | reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae | Homo sapiens |
Y505A | inactive mutant enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0081 | - |
(2E)-2-decenoyl-CoA | wild type, pH 8 | Homo sapiens | |
0.0089 | - |
(2E)-2-decenoyl-CoA | wild type, pH 5 | Homo sapiens | |
0.0092 | - |
(2E)-2-decenoyl-CoA | mutant enzyme E366A, pH 5 | Homo sapiens | |
0.0131 | - |
(2E)-2-decenoyl-CoA | mutant enzyme E366A, pH 8 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | peroxisomal hydratase 2 together with (3R)-hydroxyacyl-CoA dehydrogenase, and hydratase 1 together with (3S)-hydroxyacyl-CoA dehydrogenase, are present as multifunctional enzymes. When present simultaneously in peroxisomes, beta-oxidation has two stereochemical possibilities | Homo sapiens | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, HsMFE-2(dhdelta), HsMFE-2(dhdelta, E366A), HsMFE-2(dhdelta, D510A), SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | peroxisomal hydratase 2 together with (3R)-hydroxyacyl-CoA dehydrogenase, and peroxisomal hydratase 1 together with (3S)-hydroxyacyl-CoA dehydrogenase, are present as multifunctional enzymes. When present simultaneously in peroxisomes, beta-oxidation has two stereochemical possibilities | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
strain HsMFE-2(D490A) | Homo sapiens |
0.16 | - |
strain HsMFE-2(G16S) | Homo sapiens |
0.2 | - |
strain HsMFE-2(H532A) | Homo sapiens |
0.21 | - |
strain HsMFE-2(D370A), strain HsMFE-2(H406A) | Homo sapiens |
0.24 | - |
strain HsMFE-2(Y410A) | Homo sapiens |
0.26 | - |
strain HsMFE-2(D517A), strain HsMFE-2(E408A) | Homo sapiens |
0.4 | - |
strain UTL-7A | Homo sapiens |
0.54 | - |
strain HsMFE-2 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | peroxisomal hydratase 2 together with (3R)-hydroxyacyl-CoA dehydrogenase, and peroxisomal hydratase 1 together with (3S)-hydroxyacyl-CoA dehydrogenase, are present as multifunctional enzymes. When present simultaneously in peroxisomes, beta-oxidation has two stereochemical possibilities | Homo sapiens | ? | - |
? | |
trans-2-decenoyl-CoA + H2O | - |
Homo sapiens | (3R)-3-hydroxydecanoyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, HsMFE-2(dhdelta), HsMFE-2(dhdelta, E366A), HsMFE-2(dhdelta, D510A), SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
2-enoyl-CoA hydratase 2 | - |
Homo sapiens |
2-enoyl-CoA hydratase 2 | domain in human MFE-2 | Homo sapiens |
MFE-2 | peroxisomal hydratase 2 together with (3R)-hydroxyacyl-CoA dehydrogenase is present as multifunctional enzyme | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
(2E)-2-decenoyl-CoA | mutant enzyme E366A, pH 5 | Homo sapiens | |
17.9 | - |
(2E)-2-decenoyl-CoA | mutant enzyme E366A, pH 8 | Homo sapiens | |
105 | - |
(2E)-2-decenoyl-CoA | wild type enzyme, pH 5 | Homo sapiens | |
196 | - |
(2E)-2-decenoyl-CoA | wild type enzyme, pH 8 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH dependence experiment is performed in 200 mM potassium phosphate buffer at pH values varying from 5 to 10 | Homo sapiens |