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Literature summary for 4.2.1.116 extracted from
- Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea (2016), Metab. Eng., 38, 446-463 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene hpcd, recombinant expression of His-tagged enzyme HPCD in Escherichi coli strain Rosetta 2 (DE3), coexpression with 3-hydroxypropionyl-CoA synthetase, acryloyl-CoA reductase, and succinic semialdehyde reductase. Yeast two-hybrid assay for protein interaction analysis of 3-hydroxypropionyl-CoA dehydratase and 3-hydroxypropionyl-CoA synthetase (HPCS-HPCD) | Metallosphaera sedula |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic modelling, overview | Metallosphaera sedula | |
| 0.0251 | - |
3-hydroxypropanoyl-CoA | recombinant enzyme, pH 6.5, 65°C | Metallosphaera sedula |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 220000 | - |
recombinant enzyme, gel filtration | Metallosphaera sedula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropanoyl-CoA | Metallosphaera sedula | - |
acryloyl-CoA + H2O | - |
r | |
| 3-hydroxypropanoyl-CoA | Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | - |
acryloyl-CoA + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metallosphaera sedula | A4YI89 | - |
- |
| Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | A4YI89 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme HPCD from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography | Metallosphaera sedula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropanoyl-CoA | - |
Metallosphaera sedula | acryloyl-CoA + H2O | - |
r | |
| 3-hydroxypropanoyl-CoA | - |
Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | acryloyl-CoA + H2O | - |
r | |
| additional information | coupled assay of enzyme HPCD with 3-hydroxypropionyl-CoA synthetase (HPCS) and acryloyl-CoA reductase (ACR) | Metallosphaera sedula | ? | - |
? | |
| additional information | coupled assay of enzyme HPCD with 3-hydroxypropionyl-CoA synthetase (HPCS) and acryloyl-CoA reductase (ACR) | Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | 8 * 28000, recombinant enzyme, SDS-PAGE | Metallosphaera sedula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPCD | - |
Metallosphaera sedula |
| Msed_2001 | - |
Metallosphaera sedula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
coupled assay at | Metallosphaera sedula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
coupled assay at | Metallosphaera sedula |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | yeast two-hybrid assay for protein interaction analysis of 3-hydroxypropionyl-CoA dehydratase and 3-hydroxypropionyl-CoA synthetase (HPCS-HPCD), method optimization, metabolic engineering, overview | Metallosphaera sedula |
| physiological function | 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO2 in extremely thermoacidophilic archaea and holds promise for metabolic engineering because of its thermostability and potentially rapid pathway kinetics. Modelling of the reactions and kinetics of five of the cycle enzymes: malonyl-CoA/succinyl-CoA reductase, 3-hydroxypropionyl-CoA synthetase, 3-hydroxypropionyl-CoA dehydratase, acryloyl-CoA reductase, and succinic semialdehyde reductase, that are recombinantly expressed in Escherichia coli. Reaction kinetics model of the 3HP/4HB cycle, overview | Metallosphaera sedula |
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