Crystallization (Comment) | Organism |
---|---|
purified native isozyme alphaCA1, 0.002 ml of 20 mg/ml protein in 25 mM Tris-HCl, pH 7.5, is mixed with 0.002 ml of reservoir solution, containing 1.0 M ammonium sulfate and 100 mM Tris-HCl, pH 8.5, and equilibrated against 0.7 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.88 A resolution, MAD method | Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | two Zn atoms in the alphaCA1 crystal asymmetric unit | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
isozyme alphaCA1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange and hydrophobic interaction chromatography, followed by gel filtration | Chlamydomonas reinhardtii |
Subunits | Comment | Organism |
---|---|---|
dimer | most alphaCA enzymes are monomeric, but alphaCA1 from Chlamydomonas reinhardtii is a dimer that is uniquely stabilized by disulfide bonds, crystal structure determination and analysis | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
alphaCA1 | - |
Chlamydomonas reinhardtii |
carbonic anhydrase | - |
Chlamydomonas reinhardtii |