Cloned (Comment) | Organism |
---|---|
wild-type and variant carbonic anhydrases heterologously produced in Escherichia coli BL21(DE3) | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
D34A | site-directed mutagenesis | Methanothermobacter thermautotrophicus |
D34A/R36A | site-directed mutagenesis | Methanothermobacter thermautotrophicus |
D34E | site-directed mutagenesis | Methanothermobacter thermautotrophicus |
R36A | site-directed mutagenesis | Methanothermobacter thermautotrophicus |
R36K | site-directed mutagenesis | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
CO2 | pH 8.5, 25°C, mutant D34E | Methanothermobacter thermautotrophicus | |
2.7 | - |
CO2 | pH 8.5, 25°C, wild-type, MOPS buffer | Methanothermobacter thermautotrophicus | |
2.9 | - |
CO2 | pH 8.5, 25°C, wild-type | Methanothermobacter thermautotrophicus | |
3.5 | - |
CO2 | pH 8.5, 25°C, mutant D34A, MOPS buffer | Methanothermobacter thermautotrophicus | |
3.6 | - |
CO2 | pH 8.5, 25°C, mutant D34A | Methanothermobacter thermautotrophicus | |
4.2 | - |
CO2 | pH 8.5, 25°C, wild-type, imidazole buffer | Methanothermobacter thermautotrophicus | |
5.8 | - |
CO2 | pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer | Methanothermobacter thermautotrophicus | |
7.2 | - |
CO2 | pH 8.5, 25°C, mutant R36K | Methanothermobacter thermautotrophicus | |
9.2 | - |
CO2 | pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer | Methanothermobacter thermautotrophicus | |
15.3 | - |
CO2 | pH 8.5, 25°C, mutant D34A, imidazole buffer | Methanothermobacter thermautotrophicus | |
35.3 | - |
CO2 | pH 8.5, 25°C, mutant R36A, MOPS buffer | Methanothermobacter thermautotrophicus | |
36.2 | - |
CO2 | pH 8.5, 25°C, mutant R36A | Methanothermobacter thermautotrophicus | |
47.1 | - |
CO2 | pH 8.5, 25°C, mutant R36A, imidazole buffer | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-hydroxide mechanism of catalysis | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Methanothermobacter thermautotrophicus | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Methanothermobacter thermautotrophicus | CO2 + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
beta-class carbonic anhydrase | - |
Methanothermobacter thermautotrophicus |
Cab | - |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
CO2 | pH 8.5, 25°C, mutant R36A, MOPS buffer | Methanothermobacter thermautotrophicus | |
30 | - |
CO2 | pH 8.5, 25°C, mutant R36A, imidazole buffer | Methanothermobacter thermautotrophicus | |
46 | - |
CO2 | pH 8.5, 25°C, mutant R36A | Methanothermobacter thermautotrophicus | |
92 | - |
CO2 | pH 8.5, 25°C, mutant R36K | Methanothermobacter thermautotrophicus | |
260 | - |
CO2 | pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer | Methanothermobacter thermautotrophicus | |
520 | - |
CO2 | pH 8.5, 25°C, mutant D34A/R36A | Methanothermobacter thermautotrophicus | |
1100 | - |
CO2 | pH 8.5, 25°C, mutant D34A, MOPS buffer | Methanothermobacter thermautotrophicus | |
1500 | - |
CO2 | pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer | Methanothermobacter thermautotrophicus | |
3600 | - |
CO2 | pH 8.5, 25°C, mutant D34E | Methanothermobacter thermautotrophicus | |
4200 | - |
CO2 | pH 8.5, 25°C, mutant D34A | Methanothermobacter thermautotrophicus | |
9100 | - |
CO2 | pH 8.5, 25°C, mutant D34A, imidazole buffer | Methanothermobacter thermautotrophicus | |
11000 | - |
CO2 | pH 8.5, 25°C, wild-type, MOPS buffer | Methanothermobacter thermautotrophicus | |
13000 | - |
CO2 | pH 8.5, 25°C, wild-type, imidazole buffer | Methanothermobacter thermautotrophicus | |
24000 | - |
CO2 | pH 8.5, 25°C, wild-type | Methanothermobacter thermautotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 9 | - |
Methanothermobacter thermautotrophicus |