Cloned (Comment) | Organism |
---|---|
alpha-carbonic anhydrase gene cloned and sequenced, truncated gene expressed in Escherichia coli BL21(DE3) | Helicobacter pylori |
General Stability | Organism |
---|---|
enzyme appears to denature with a midpoint of a structural transition near 2.1 M denaturant guanidine hydrochloride | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-acetylamido-1,3,4-thiadiazole-5-sulfonamide | acetazolamide | Helicobacter pylori | |
thiocyanate | - |
Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
CO2 | pH 8.9, 25°C | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28280 | - |
amino acid sequence | Helicobacter pylori |
29000 | - |
SDS-PAGE | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Helicobacter pylori | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
ATCC type strain 628572 | - |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl acetate + H2O | - |
Helicobacter pylori | 2-nitrophenol + acetate | - |
r | |
4-nitrophenyl acetate + H2O | - |
Helicobacter pylori | 4-nitrophenol + acetate | - |
r | |
H2CO3 | - |
Helicobacter pylori | CO2 + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
alpha-carbonic anhydrase | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240000 | - |
CO2 | pH 8.9, 25°C | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9 | - |
Helicobacter pylori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
thiocyanate | pH 7.5, 25°C | Helicobacter pylori |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Helicobacter pylori | calculated from amino acid composition | - |
8.8 |
Helicobacter pylori | isoelectric focusing | - |
9 |