Cloned (Comment) | Organism |
---|---|
bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a | Paraburkholderia xenovorans |
Protein Variants | Comment | Organism |
---|---|---|
G322A | channels acetaldehyde with similar efficiency to wild-type, 30% lowered efficiency of isobutyraldehyde channeling | Paraburkholderia xenovorans |
G322F | unable to channel either acetaldehyde or propionaldehyde | Paraburkholderia xenovorans |
G322L | unable to channel either acetaldehyde or propionaldehyde | Paraburkholderia xenovorans |
G323A | channels acetaldehyde with similar efficiency to wild-type, unable to channel isobutyraldehyde | Paraburkholderia xenovorans |
G323F | unable to channel either acetaldehyde or propionaldehyde | Paraburkholderia xenovorans |
G323L | 63% channeling efficiency for acetaldehyde, unable to channel propionaldehyde | Paraburkholderia xenovorans |
H20A | reduction of acetaldehyde and propionaldehyde channeling by more than 70% | Paraburkholderia xenovorans |
L89A | reduction in channeling efficiency of 30% for all aldehydes tested | Paraburkholderia xenovorans |
additional information | all mutations created by site specific mutagenesis | Paraburkholderia xenovorans |
Y290F | reduction in channeling efficiency of 30% for all aldehydes tested | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
4-hydroxy-2-oxopentanoate | G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.06 | - |
4-hydroxy-2-oxopentanoate | G323A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.081 | - |
4-hydroxy-2-oxopentanoate | G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.089 | - |
4-hydroxy-2-oxopentanoate | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.09 | - |
4-hydroxy-2-oxopentanoate | G322F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.117 | - |
4-hydroxy-2-oxohexanoate | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.13 | - |
4-hydroxy-2-oxopentanoate | G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.14 | - |
4-hydroxy-2-oxopentanoate | G322L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.158 | - |
4-hydroxy-2-oxopentanoate | wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.16 | - |
4-hydroxy-2-oxopentanoate | G323L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.18 | - |
4-hydroxy-2-oxopentanoate | G322A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.216 | - |
4-hydroxy-2-oxopentanoate | G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.3 | - |
4-hydroxy-2-oxohexanoate | G322F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.35 | - |
4-hydroxy-2-oxohexanoate | G322L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.36 | - |
4-hydroxy-2-oxohexanoate | G323A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.37 | - |
4-hydroxy-2-oxohexanoate | G323L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.52 | - |
4-hydroxy-2-oxohexanoate | G322A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.94 | - |
4-hydroxy-2-oxopentanoate | G323F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.1 | - |
4-hydroxy-2-oxohexanoate | G323F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxohexanoate | 4-hydroxy-2-oxohexanoate i.e. HOHA | Paraburkholderia xenovorans | propionaldehyde + pyruvate | - |
? | |
4-hydroxy-2-oxopentanoate | 4-hydroxy-2-oxopentanoate i.e. HOPA | Paraburkholderia xenovorans | acetaldehyde + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BphI | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-hydroxy-2-oxopentanoate | G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.27 | - |
4-hydroxy-2-oxohexanoate | G323L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.286 | - |
4-hydroxy-2-oxopentanoate | G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.34 | - |
4-hydroxy-2-oxopentanoate | G323A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.45 | - |
4-hydroxy-2-oxopentanoate | G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.46 | - |
4-hydroxy-2-oxohexanoate | G323A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.64 | - |
4-hydroxy-2-oxopentanoate | G323L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.72 | - |
4-hydroxy-2-oxopentanoate | G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.79 | - |
4-hydroxy-2-oxopentanoate | wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C | Paraburkholderia xenovorans | |
0.94 | - |
4-hydroxy-2-oxohexanoate | G322L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.05 | - |
4-hydroxy-2-oxohexanoate | G322F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.06 | - |
4-hydroxy-2-oxopentanoate | G322F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.09 | - |
4-hydroxy-2-oxopentanoate | G322L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.3 | - |
4-hydroxy-2-oxohexanoate | G323F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
1.9 | - |
4-hydroxy-2-oxohexanoate | G322A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
2.4 | - |
4-hydroxy-2-oxopentanoate | G322A mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
3.9 | - |
4-hydroxy-2-oxohexanoate | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.07 | - |
4-hydroxy-2-oxopentanoate | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.3 | - |
4-hydroxy-2-oxopentanoate | G323F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is part of the BphI-BphJ-complex, an aldolase-dehydrogenase complex from the polychlorinated biphenyl degradation pathway | Paraburkholderia xenovorans |