Crystallization (Comment) | Organism |
---|---|
purified enzyme complex of 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating), protein solution contains 9 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 1 mM DTT, and 2 mM NAD+, mixing of equal volumes of 0.001 ml of protein and reservoir solutions, the latter contains 15% PEG 8000, 100 mM ammonium sulfate, and 100 mM PIPES, pH 7.5, 3 days, soaking of crystals in 5 mM samarium acetate and 0.25 mM PCMBS, X-ray diffraction structure determination and analysis at 2.1 A resolution | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxovalerate | Pseudomonas sp. | last but one step in meta-cleavage pathway for catechol degradation | pyruvate + acetaldehyde | - |
? | |
4-hydroxy-2-oxovalerate | Pseudomonas sp. CF 600 | last but one step in meta-cleavage pathway for catechol degradation | pyruvate + acetaldehyde | - |
? | |
additional information | Pseudomonas sp. | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | ? | - |
? | |
additional information | Pseudomonas sp. CF 600 | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase/aldehyde dehydrogenase (acylating), EC 1.2.1.10 | - |
Pseudomonas sp. CF 600 | - |
dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase/aldehyde dehydrogenase (acylating), EC 1.2.1.10 | - |
Purification (Comment) | Organism |
---|---|
co-purification with aldehyde dehydrogenase, EC 1.2.1.10, to homogeneity in a 3-step chromatographic procedure involving NAD+ affinity chromatography | Pseudomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate | reaction mechanism | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxovalerate | - |
Pseudomonas sp. | pyruvate + acetaldehyde | - |
? | |
4-hydroxy-2-oxovalerate | last but one step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. | pyruvate + acetaldehyde | - |
? | |
4-hydroxy-2-oxovalerate | - |
Pseudomonas sp. CF 600 | pyruvate + acetaldehyde | - |
? | |
4-hydroxy-2-oxovalerate | last but one step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. CF 600 | pyruvate + acetaldehyde | - |
? | |
additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA, product channeling mechanism | Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation | Pseudomonas sp. CF 600 | ? | - |
? | |
additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA, product channeling mechanism | Pseudomonas sp. CF 600 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme forms a heterodimer of 71 kDa with the aldehyde dehydrogenase, EC 1.2.1.10 | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
DmpFG | - |
Pseudomonas sp. |