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Literature summary for 4.1.2.63 extracted from

  • Foulon, V.; Antonenkov, V.D.; Croes, K.; Waelkens, E.; Mannaerts, G.P.; Van Veldhoven, P.P.; Casteels, M.
    Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids (1999), Proc. Natl. Acad. Sci. USA, 96, 10039-10044 .
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 2-hydroxy-3-methylhexadecanoyl-CoA at pH 7.5 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Rattus norvegicus 5777
-
peroxisome
-
 Homo sapiens 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum activity in the presence of 0.8 mM Mg2+ Rattus norvegicus
Mg2+ optimum activity in the presence of 0.8 mM Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxyphytanoyl-CoA Rattus norvegicus
-
 pristanal + formyl-CoA
-
 ?
2-hydroxyphytanoyl-CoA Homo sapiens
-
 pristanal + formyl-CoA
-
 ?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA Rattus norvegicus
-
 a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA Homo sapiens
-
 a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UJ83
-
-
Rattus norvegicus Q8CHM7
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, phosphocellulose chromatography, and Ultrogel AcA-34 gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney highest expression in liver and kidney Homo sapiens
-
liver
-
 Rattus norvegicus
-
liver highest expression in liver and kidney Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-3-methylhexadecanoyl-CoA
-
 Rattus norvegicus 2-methylpentadecanal + formyl-CoA
-
 ?
2-hydroxy-3-methylhexadecanoyl-CoA
-
 Homo sapiens 2-methylpentadecanal + formyl-CoA
-
 ?
2-hydroxyphytanoyl-CoA
-
 Rattus norvegicus pristanal + formyl-CoA
-
 ?
2-hydroxyphytanoyl-CoA
-
 Homo sapiens pristanal + formyl-CoA
-
 ?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA
-
 Rattus norvegicus a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA
-
 Homo sapiens a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 63000, SDS-PAGE Rattus norvegicus
homotetramer 4 * 63000, SDS-PAGE Homo sapiens
homotetramer 4 * 63732, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
2-HPCL
-
 Rattus norvegicus
2-HPCL
-
 Homo sapiens
2-hydroxyphytanoyl-CoA lyase
-
 Rattus norvegicus
2-hydroxyphytanoyl-CoA lyase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 in Tris buffer Rattus norvegicus
7.5 8 in Tris buffer Homo sapiens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Rattus norvegicus
thiamine diphosphate dependent on Homo sapiens