Literature summary for 4.1.2.63 extracted from

Fraccascia, P.; Casteels, M.; De Schryver, E.; Van Veldhoven, P.P.
Role of thiamine pyrophosphate in oligomerisation, functioning and import of peroxisomal 2-hydroxyacyl-CoA lyase (2011), Biochim. Biophys. Acta, 1814, 1226-1233 .

Search for more literature for 4.1.2.63

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in CHO cells and Saccharomyces cerevisiae strain CB80	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D455S	the mutation within the thiamine diphosphate binding domain of the enzyme does not affect the targeting upon expression in transfected CHO cells, although enzyme activity is reduced to 5.38% of wild type activity	Homo sapiens
D455S/S456R	the mutation within the thiamine diphosphate binding domain of the enzyme does not affect the targeting upon expression in transfected CHO cells, although enzyme activity is reduced to 2.43% of wild type activity	Homo sapiens
S456R	the mutation within the thiamine diphosphate binding domain of the enzyme does not affect the targeting upon expression in transfected CHO cells, although enzyme activity is reduced to 2.17% of wild type activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Homo sapiens	5777	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA	Homo sapiens	-	a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UJ83	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
500	-	recombinant wild type enzyme, with 2-hydroxy-3-methylhexadecanoyl-CoA as substrate, pH and temperature not specified in the publication	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxy-3-methylhexadecanoyl-CoA	-	Homo sapiens	3-methylpentadecanal + formyl-CoA	-	?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA	-	Homo sapiens	a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA	-	?

Subunits

Subunits	Comment	Organism
?	x * 64000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
2-hydroxyacyl-CoA lyase	-	Homo sapiens
HACL1	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Homo sapiens

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Release 2023.1 (January 2023)