Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Pseudomonas fluorescens |
Crystallization (Comment) | Organism |
---|---|
by the hanging-drop method of vapour diffusion using polyethylene glycol 20000 Da as the precipitant, 1.8 A resolution | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31007 | - |
6 * 31007, crystallization, calculated from amino acid sequence | Pseudomonas fluorescens |
186000 | - |
calculated from crystallization data | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-feruloyl-CoA + H2O | Pseudomonas fluorescens | - |
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
? | |
trans-feruloyl-CoA + H2O | Pseudomonas fluorescens AN103 | - |
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens AN103 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-feruloyl-CoA + H2O | - |
Pseudomonas fluorescens | 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
? | |
trans-feruloyl-CoA + H2O | - |
Pseudomonas fluorescens AN103 | 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 31007, crystallization, calculated from amino acid sequence | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxycinnamoyl-coenzyme A hydratase-lyase | - |
Pseudomonas fluorescens |
FCHL | - |
Pseudomonas fluorescens |
feruloyl-CoA hydratase-lyase | - |
Pseudomonas fluorescens |
HCHL | - |
Pseudomonas fluorescens |