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Literature summary for 4.1.1.61 extracted from

  • Gallert, C.; Winter, J.
    Comparison of 4-hydroxybenzoate decarboxylase, and phenol carboxylase activities in cell-free extracts of a defined 4-hydroxybenzoate and phenol-degrading anaerobic consortium (1992), Appl. Microbiol. Biotechnol., 37, 119-124.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2,3,4-Trihydroxybenzoate complete inhibition of carboxylation of phenol Desulfovibrio sp.
2,3,4-Trihydroxybenzoate complete inhibition of carboxylation of phenol Methanospirillum hungatei
2,4,6-Trihydroxybenzoate complete inhibition of carboxylation of phenol Desulfovibrio sp.
2,4,6-Trihydroxybenzoate complete inhibition of carboxylation of phenol Methanospirillum hungatei
2,4-Dihydroxybenzoate
-
Desulfovibrio sp.
2,4-Dihydroxybenzoate
-
Methanospirillum hungatei
3,4,5-Trihydroxybenzoate slight inhibition of carboxylation of phenol Desulfovibrio sp.
3,4,5-Trihydroxybenzoate slight inhibition of carboxylation of phenol Methanospirillum hungatei
3-Chloro-4-hydroxybenzoate
-
Desulfovibrio sp.
3-Chloro-4-hydroxybenzoate
-
Methanospirillum hungatei
Co2+
-
Desulfovibrio sp.
Co2+
-
Methanospirillum hungatei
EDTA
-
Desulfovibrio sp.
EDTA
-
Methanospirillum hungatei
m-Hydroxybenzoylhydrazine dichloride
-
Desulfovibrio sp.
m-Hydroxybenzoylhydrazine dichloride
-
Methanospirillum hungatei
Sodium dithionite
-
Desulfovibrio sp.
Sodium dithionite
-
Methanospirillum hungatei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
4-hydroxybenzoate carboxylase with pH-optimum 7.8 Methanospirillum hungatei
0.004
-
4-hydroxybenzoate carboxylase with pH-optimum 7.8 Desulfovibrio sp.
0.02
-
4-hydroxybenzoate carboxylase with pH-optimum 6.4 Methanospirillum hungatei
0.02
-
4-hydroxybenzoate carboxylase with pH-optimum 6.4 Desulfovibrio sp.
0.125
-
phenol
-
Methanospirillum hungatei
0.125
-
phenol
-
Desulfovibrio sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane loosely associated Methanospirillum hungatei 16020
-
membrane loosely associated Desulfovibrio sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on Methanospirillum hungatei
K+ phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on Desulfovibrio sp.
Mn2+ restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8 Methanospirillum hungatei
Mn2+ restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8 Desulfovibrio sp.
Na+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Methanospirillum hungatei
Na+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Desulfovibrio sp.
NH4+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Methanospirillum hungatei
NH4+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Desulfovibrio sp.
Rb+ can replace K+ in the phenol carboxylase and partially in both decarboxylases Methanospirillum hungatei
Rb+ can replace K+ in the phenol carboxylase and partially in both decarboxylases Desulfovibrio sp.
Zn2+ compensates the loss of activity by EDTA Methanospirillum hungatei
Zn2+ compensates the loss of activity by EDTA Desulfovibrio sp.

Organism

Organism UniProt Comment Textmining
Desulfovibrio sp.
-
in an anaerobic consortium with Methanospirillum hungatei, one phenol carboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
Methanospirillum hungatei
-
in an anaerobic consortium with Desulfovibrio sp., one phenol decarboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-

Storage Stability

Storage Stability Organism
-20┬░C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months Methanospirillum hungatei
-20┬░C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months Desulfovibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,5-Trihydroxybenzoate
-
Methanospirillum hungatei Pyrogallol + CO2
-
?
3,4,5-Trihydroxybenzoate
-
Desulfovibrio sp. Pyrogallol + CO2
-
?
3,5-Dihydroxybenzoate
-
Methanospirillum hungatei 1,3-Dihydroxybenzene + CO2
-
?
3,5-Dihydroxybenzoate
-
Desulfovibrio sp. 1,3-Dihydroxybenzene + CO2
-
?
3,5-Dimethoxy-4-hydroxybenzoate
-
Methanospirillum hungatei 1,3-Dimethoxy-2-hydroxybenzene + CO2
-
?
3,5-Dimethoxy-4-hydroxybenzoate
-
Desulfovibrio sp. 1,3-Dimethoxy-2-hydroxybenzene + CO2
-
?
3-Chloro-4-hydroxybenzoate
-
Methanospirillum hungatei 1-Chloro-2-hydroxybenzene + CO2
-
?
3-Chloro-4-hydroxybenzoate
-
Desulfovibrio sp. 1-Chloro-2-hydroxybenzene + CO2
-
?
4-Hydroxybenzoate
-
Methanospirillum hungatei Phenol + CO2
-
?
4-Hydroxybenzoate
-
Desulfovibrio sp. Phenol + CO2
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
enzyme with pH-optimum of pH 7.8 Methanospirillum hungatei
37
-
enzyme with pH-optimum of pH 7.8 Desulfovibrio sp.
41
-
enzyme with pH-optimum of pH 6.4 Methanospirillum hungatei
41
-
enzyme with pH-optimum of pH 6.4 Desulfovibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
Methanospirillum hungatei
6.4
-
-
Desulfovibrio sp.
6.5
-
phenol carboxylation Methanospirillum hungatei
6.5
-
phenol carboxylation Desulfovibrio sp.
7.8
-
-
Methanospirillum hungatei
7.8
-
-
Desulfovibrio sp.