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Literature summary for 4.1.1.15 extracted from

  • Yang, S.; Lin, Q.; Lu, Z.-X.; Lu, F.-X.; Bie, X.-M.; Zou, X.-K.; Sun, L.-J.
    Characterization of a novel glutamate decarboxylase from Streptococcus salivarius ssp. thermophilus Y2 (2008), J. Chem. Technol. Biotechnol., 83, 855-861 .
No PubMed abstract available
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene gadB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) Streptococcus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 complete inhibition at 5 mM Streptococcus thermophilus
CaCl2 95.4% remaining activity at 5 mM Streptococcus thermophilus
CoCl2 37.9% remaining activity at 5 mM; 62.1% inhibition at 5 mM Streptococcus thermophilus
CuSO4 92.4% inhibition at 5 mM Streptococcus thermophilus
FeSO4 91.9% inhibition at 5 mM Streptococcus thermophilus
KCl 5.8% inhibition at 5 mM; 94.2% remaining activity at 5 mM Streptococcus thermophilus
LiCl 6.1% inhibition at 5 mM; 93.9% remaining activity at 5 mM Streptococcus thermophilus
MnSO4 87.9% inhibition at 5 mM Streptococcus thermophilus
Na2SO4 9.8% inhibition at 5 mM Streptococcus thermophilus
ZnSO4 80.4% inhibition at 5 mM Streptococcus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Lineweaver-Burk plots Streptococcus thermophilus
2.3
-
 L-glutamate pH 4.0, 55°C Streptococcus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
BaCl2 activates to 122.5% at 5 mM Streptococcus thermophilus
MgCl2 98.7% of control activity at 5 mM Streptococcus thermophilus
NaCl 101.6% of control activity at 5 mM Streptococcus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103570
-
 gel filtration Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Streptococcus thermophilus
-
 4-aminobutanoate + CO2
-
 ir
L-glutamate Streptococcus thermophilus DSM 20617
-
 4-aminobutanoate + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus Q0GE18 i.e. Streptococcus thermophilus
-
Streptococcus thermophilus DSM 20617 Q0GE18 i.e. Streptococcus thermophilus
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme GAD 22fold by ammonium sulfate fractionation, dialysis, precipitation in N-methylpiperazine, anion exchange and hydrophobic interaction chromatography, and gel filtration Streptococcus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
103.5
-
 purified native enzyme, pH 4.0, 55°C Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Streptococcus thermophilus 4-aminobutanoate + CO2
-
 ir
L-glutamate irreversible alpha-decarboxylation of L-glutamate Streptococcus thermophilus 4-aminobutanoate + CO2
-
 ir
L-glutamate
-
 Streptococcus thermophilus DSM 20617 4-aminobutanoate + CO2
-
 ir
L-glutamate irreversible alpha-decarboxylation of L-glutamate Streptococcus thermophilus DSM 20617 4-aminobutanoate + CO2
-
 ir
additional information the enzyme is highly substrate specific. No activity with D-glutamate and no activity with L-Asp, L-Ser, L-His, Gly, L-Thr, L-Ala, L-Arg, L-Tyr, L-Cys, L-Val, L-Met, L-Trp, L-Phe, L-Ile, L-Leu, L-Lys, and L-Pro Streptococcus thermophilus ?
-
 ?
additional information the enzyme is highly substrate specific. No activity with D-glutamate and no activity with L-Asp, L-Ser, L-His, Gly, L-Thr, L-Ala, L-Arg, L-Tyr, L-Cys, L-Val, L-Met, L-Trp, L-Phe, L-Ile, L-Leu, L-Lys, and L-Pro Streptococcus thermophilus DSM 20617 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 46850, SDS-PAGE Streptococcus thermophilus
More the N-terminal amino acid sequence of GAD is NH2-MNEKLFREI Streptococcus thermophilus

Synonyms

Synonyms Comment Organism
GAD
-
 Streptococcus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Streptococcus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Streptococcus thermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Streptococcus thermophilus

General Information

General Information Comment Organism
physiological function gamma-aminobutyric acid with several physiological functions is biosynthesized via the irreversible alpha-decarboxylation of L-glutamate catalysed by glutamate decarboxylase (GAD). Streptococcus salivarius ssp. thermophilus is widely applied to the dairy Streptococcus thermophilus