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Literature summary for 4.1.1.15 extracted from

  • Okai, N.; Takahashi, C.; Hatada, K.; Ogino, C.; Kondo, A.
    Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase (2014), AMB Express, 4, 20 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene gadB, recombinant expression in Corynebacterium glutamicum strain ATCC 13032 with or without gene pknG knockout Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the Escherichia coli glutamate decarboxylase is expressed in Corynebacterium glutamicum for production of gamma-aminobutyric acid (GABA), a building block of the biodegradable plastic polyamide 4. Disruption of gene pknG, encoding serine/threonine protein kinase G (EC 2.7.11.1), enhances recombinant production of gamma-aminobutyric acid through reduction of the 2-oxoglutarate dehydrogenase complex (ODHC) activity regulation, PknG catalyzes the phosphorylation of OdhI, a 15 kDa subunit of ODHC, which reverses the inhibition of the ODHC activity. Strain GAD produces 13.06 g/l of GABA in 120 hours, consuming 83.62 g/l of glucose, strain GADDELTApknG produces 0.272 g/l of GABA in 120 hours, consuming less glucose Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Escherichia coli
-
 4-aminobutanoate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli A5YKJ2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Escherichia coli 4-aminobutanoate + CO2
-
 ?
L-glutamate alpha-decarboxylation Escherichia coli 4-aminobutanoate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
GAD B
-
 Escherichia coli
GadB
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli