Any feedback?
Literature summary for 4.1.1.15 extracted from
- Cloning and characterization of a maize cDNA encoding glutamate decarboxylase (2010), Plant Mol. Biol. Rep., 28, 620-626.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Zea mays |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56200 | - |
x * 56200, calculated from amino acid sequence | Zea mays |
| 61000 | - |
x * 61000, His-tagged enzyme, SDS-PAGE | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
inbred line DH4866 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His binding resin column chromatography | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corn ear | the enzyme is most abundantly expressed in the corn ears | Zea mays | - |
| leaf | - |
Zea mays | - |
| root | - |
Zea mays | - |
| stem | - |
Zea mays | - |
| tassel | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Zea mays | 4-aminobutanoate + CO2 | - |
ir |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56200, calculated from amino acid sequence | Zea mays |
| ? | x * 61000, His-tagged enzyme, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD1 | - |
Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | - |
Zea mays | |
| pyridoxal 5'-phosphate | - |
Zea mays | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Zea mays | calculated from amino acid sequence | - |
5.4 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Zea mays | GAD1 gene is upregulated and GAD activity is increased in the leaves and roots after treatment with 0.1 mM ABA, 0.1 mM methyl jasmonate, 0.8% (w/v) NaCl, 20% (w/v) polyethylene glycol, or cold stress | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | GAD1 plays an important role in responses to abiotic factors and hormone treatments | Zea mays |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
