Literature summary for 4.1.1.101 extracted from

Schuemann, C.; Michlmayr, H.; Eder, R.; Del Hierro, A.M.; Kulbe, K.D.; Mathiesen, G.; Nguyen, T.H.
Heterologous expression of Oenococcus oeni malolactic enzyme in Lactobacillus plantarum for improved malolactic fermentation (2012), AMB Express, 2, 19.

Search for more literature for 4.1.1.101

Application

Application	Comment	Organism
food industry	expression of malolactic enzyme from Oenococcus oeni in the host strain Lactobacillus plantarum WCFS1. Under conditions with L-malic acid as the only energy source and in presence of Mn2+ and NAD+, the recombinant Lactobacillus plantarum and the wild-type strain convert 85% (2.5 g/l) and 51% (1.5 g/l), respectively, of L-malic acid in 3.5 days. The recombinant Lactobacillus plantarum cells convert in a modified wine 15% (0.4 g/l) of initial L-malic acid concentration in 2 days	Oenococcus oeni

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 62300, calculated, x * 60000, SDS-PAGE	Oenococcus oeni
62300	-	x * 62300, calculated, x * 60000, SDS-PAGE	Oenococcus oeni

Organism

Organism	UniProt	Comment	Textmining
Oenococcus oeni	Q5S3L3	-	-

Subunits

Subunits	Comment	Organism
?	x * 62300, calculated, x * 60000, SDS-PAGE	Oenococcus oeni

Synonyms

Synonyms	Comment	Organism
mleA	-	Oenococcus oeni

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Oenococcus oeni

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	-	72% of maximum activity	Oenococcus oeni

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Oenococcus oeni

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	6.5	-	Oenococcus oeni

General Information

General Information	Comment	Organism
physiological function	recombinant Lactobacillus plantarum cells expressing malolactic enzyme accelerate the malolactic fermentation	Oenococcus oeni

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Release 2023.1 (January 2023)