Literature summary for 3.8.1.5 extracted from

Stsiapanava, A.; Koudelakova, T.; Lapkouski, M.; Pavlova, M.; Damborsky, J.; Smatanova, I.K.
Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis (2008), Acta Crystallogr. Sect. F, 64, 137-140.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Rhodococcus rhodochrous

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzymes C176Y, I135F, and I135F/C176Y are crystallized by sitting drop vapour diffusion method, using either 80 mM bicine pH 9.0, 8% (v/v) PEG 8000, and 80 mM MgCl2 or 25% (v/v) PEG 4000, 8% 2-propanol, and 100 mM sodium acetate	Rhodococcus rhodochrous

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus rhodochrous	P0A3G2	NCIMB 13064	-
Rhodococcus rhodochrous NCIMB 13064	P0A3G2	NCIMB 13064	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Ni-chelating affinity resin chromatography and Superdex 75 gel filtration	Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2,3-trichloropropane + H2O	-	Rhodococcus rhodochrous	2,3-dichlorpropane-1-ol + chloride	-	ir
1,2,3-trichloropropane + H2O	-	Rhodococcus rhodochrous NCIMB 13064	2,3-dichlorpropane-1-ol + chloride	-	ir

Synonyms

Synonyms	Comment	Organism
DhaA	-	Rhodococcus rhodochrous

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Release 2023.1 (January 2023)