Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.5 extracted from

  • Pries, F.; Kingma, J.; Krooshof, G.H.; Jeronimus-Stratingh, C.M.; Bruins, A.; Janssen, D.B.
    Histidine 289 is essential for hydrolysis of the alkyl-enzyme intermediate of haloalkane dehalogenase (1995), J. Biol. Chem., 270, 10405-10411.
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
GJ10 mutant H289Q, expression in Escherichia coli Xanthobacter autotrophicus

Protein Variants

Protein Variants Comment Organism
H289Q GJ10, mutant with 660fold reduced activity with substrate 1,2-dibromoethane Xanthobacter autotrophicus

Inhibitors

Inhibitors Comment Organism Structure
halide GJ10 mutant H289Q blocked by bromide in intermediate state Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 1,2-Dibromoethane GJ10 Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-dichloroethane + H2O Xanthobacter autotrophicus
-
 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O Xanthobacter autotrophicus GJ10
-
 2-chloroethanol + chloride
-
 ?
n-haloalkanes + H2O Xanthobacter autotrophicus broad substrate range primary alcohols + halide
-
 ?
n-haloalkanes + H2O Xanthobacter autotrophicus GJ10 broad substrate range primary alcohols + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
 GJ10
-
Xanthobacter autotrophicus GJ10
-
 GJ10
-

Purification (Commentary)

Purification (Comment) Organism
GJ10 mutant H289Q Xanthobacter autotrophicus

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction Xanthobacter autotrophicus
a
1-haloalkane + H2O = a primary alcohol + halide two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis Xanthobacter autotrophicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-bromoethanol + bromide
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus alcohol + halide
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus GJ10 alcohol + halide
-
 ?
n-haloalkanes + H2O broad substrate range Xanthobacter autotrophicus primary alcohols + halide
-
 ?
n-haloalkanes + H2O broad substrate range Xanthobacter autotrophicus GJ10 primary alcohols + halide
-
 ?