Any feedback?
Literature summary for 3.6.1.74 extracted from
- A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme (1999), Nucleic Acids Res., 27, 2181-2188 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CET1, recombinant expression of wild-type His7-tagged Cet1 and of C-terminally truncated mutant Cet1 (265-549) in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
| gene CTL1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of the GFP-tagged enzyme in Saccharomyces cerevisiae strain YSB613 cells in nucleus and cytoplasm, recombinant expression of His7-tagged Cet1 in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | enzyme CTL1 is completely inactive on trimer RNA in presence of Mn2+, and enzyme CTL1 exhibits ATPase activity only in presence of Mn2+ | Saccharomyces cerevisiae |  |
| additional information | no inhibition pf Cet1 by 10 mM sodium vanadate; no inhibition pf CTL1 by 10 mM sodium vanadate | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates optimally at 5 mM Mg2+, inhibition above | Saccharomyces cerevisiae | |
| Mg2+ | activates optimally at up to 30 mM | Saccharomyces cerevisiae | |
| Mn2+ | enzyme CTL1 is completely inactive on trimer RNA in presence of Mn2+, and enzyme CTL1 exhibits ATPase activity only in presence of Mn2+ | Saccharomyces cerevisiae | |
| additional information | Mg2+ and Mn2+ can alter the enzyme substrate specificity, overview | Saccharomyces cerevisiae | |
| additional information | the substrate specificity of Cet1 is unaltered by Mg2+ or Mn2+ | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a 5'-triphospho-[mRNA] + H2O | Saccharomyces cerevisiae | - |
a 5'-diphospho-[mRNA] + phosphate | - |
? | |
| a 5'-triphospho-[mRNA] + H2O | Saccharomyces cerevisiae ATCC 204508 | - |
a 5'-diphospho-[mRNA] + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | O13297 | - |
- |
| Saccharomyces cerevisiae | Q03220 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | O13297 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | Q03220 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His7-tagged Cet1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, cation exchange chromatography, dialysis, and anion exchange chromatography | Saccharomyces cerevisiae |
| recombinant His7-tagged wild-type and truncated mutant CTL1s from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and cation exchange chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a 5'-triphospho-[mRNA] + H2O | - |
Saccharomyces cerevisiae | a 5'-diphospho-[mRNA] + phosphate | - |
? | |
| a 5'-triphospho-[mRNA] + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | a 5'-diphospho-[mRNA] + phosphate | - |
? | |
| additional information | enzyme Cet1 releases the gamma-phosphate from the terminal ATP of RNA | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | recombinant CTL1 releases the gamma-phosphate from the 5'-end of RNA to produce a diphosphate terminus. The enzyme is specific for polynucleotide RNA in the presence of magnesium, but becomes specific for nucleotide triphosphates in the presence of manganese. No activity with [gamma32P]ATP-terminated pppA | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | enzyme Cet1 releases the gamma-phosphate from the terminal ATP of RNA | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
| additional information | recombinant CTL1 releases the gamma-phosphate from the 5'-end of RNA to produce a diphosphate terminus. The enzyme is specific for polynucleotide RNA in the presence of magnesium, but becomes specific for nucleotide triphosphates in the presence of manganese. No activity with [gamma32P]ATP-terminated pppA | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
| pppApCpC + H2O | [gamma32P]ATP-terminated trimeric RNA or [alpha32P]ATP-terminated trimeric RNA | Saccharomyces cerevisiae | ppApCpC + phosphate | - |
? | |
| pppApCpC + H2O | [gamma32P]ATP-terminated trimeric RNA or [alpha32P]ATP-terminated trimeric RNA | Saccharomyces cerevisiae ATCC 204508 | ppApCpC + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, about, enzyme CTL1, sequence calculation | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| capping enzyme RNA 5'-triphosphatase-like 1 | - |
Saccharomyces cerevisiae |
| CET1 | - |
Saccharomyces cerevisiae |
| Cet1 RNA triphosphatase | - |
Saccharomyces cerevisiae |
| Ctl1 | - |
Saccharomyces cerevisiae |
| mRNA capping enzyme | - |
Saccharomyces cerevisiae |
| RNA 5'-triphosphatase | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | inactive below pH 7.0 | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | CTL1 is the second member of the yeast RNA triphosphatase family. Enzyme CTL1 resembles the C-terminus of Cet1, which has RNA triphosphatase activity | Saccharomyces cerevisiae |
| evolution | RNA 5'-triphosphatase activity is widely distributed among prokaryotes, eukaryotes, and viruses | Saccharomyces cerevisiae |
| additional information | Mg2+ and Mn2+ can alter the enzyme substrate specificity, overview | Saccharomyces cerevisiae |
| physiological function | capping enzyme RNA 5'-triphosphatase-like 1 (CTL1) is not essential for cell viability and no genetic or physical interactions with the capping enzyme genes are observed. Enzyme CTL1 is probably involved in an RNA processing event other than mRNA capping. CTL1 has no obvious role in 5' mRNA cap formation | Saccharomyces cerevisiae |
| physiological function | cellular capping enzymes are bifunctional. The mRNA triphosphatase Cet1 part removes the gamma-phosphate from the 5'-end of RNA, and the Ceg1 part shows RNA guanylyltransferase activity and adds GMP to the resulting diphosphate end in a 5'-5'-orientation. The cap structure is then modified by one or more methyltransferases | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
