Literature summary for 3.6.1.56 extracted from

Nissink, J.W.; Bista, M.; Breed, J.; Carter, N.; Embrey, K.; Read, J.; Winter-Holt, J.J.
MTH1 substrate recognition - an example of specific promiscuity (2016), PLoS ONE, 11, e0151154 .

Search for more literature for 3.6.1.56

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Gold BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with substrates, hanging drop vapor diffusion method, using 25% (w/v) PEG3350 as precipitant	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2-(dimethylamino)-9-methyl-1,9-dihydro-6H-purin-6-one	-	Homo sapiens
2-amino-9-methyl-1,9-dihydro-6H-purin-6-one	-	Homo sapiens
2-amino-9-methyl-7,9-dihydro-1H-purine-6,8-dione	-	Homo sapiens
3-[(2-amino-6-methylpyrimidin-4-yl)oxy]propan-1-ol	-	Homo sapiens
6-(methylamino)pyrimidine-2,4(1H,3H)-dione	-	Homo sapiens
9-methyl-2-(methylamino)-1,9-dihydro-6H-purin-6-one	-	Homo sapiens
N-(1-methyl-1H-benzimidazol-5-yl)acetamide	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0076	-	8-oxo-dATP	at pH 7.4, temperature not specified in the publication	Homo sapiens
0.0113	-	8-oxo-dGTP	at pH 7.4, temperature not specified in the publication	Homo sapiens
0.0134	-	2-hydroxy-rATP	at pH 7.4, temperature not specified in the publication	Homo sapiens
0.014	-	2-hydroxy-dATP	at pH 7.4, temperature not specified in the publication	Homo sapiens
0.082	-	8-oxo-rGTP	at pH 7.4, temperature not specified in the publication	Homo sapiens
0.24	-	8-oxo-rATP	at pH 7.4, temperature not specified in the publication	Homo sapiens
0.42	-	dGTP	at pH 7.4, temperature not specified in the publication	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P36639	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and S75 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxy-dATP + H2O	-	Homo sapiens	2-hydroxy-dAMP + diphosphate	-	?
2-hydroxy-rATP + H2O	-	Homo sapiens	2-hydroxy-rAMP + diphosphate	-	?
8-oxo-dATP + H2O	-	Homo sapiens	8-oxo-dAMP + diphosphate	-	?
8-oxo-dGTP + H2O	-	Homo sapiens	8-oxo-dGMP + diphosphate	-	?
8-oxo-rATP + H2O	-	Homo sapiens	8-oxo-rAMP + diphosphate	-	?
8-oxo-rGTP + H2O	-	Homo sapiens	8-oxo-rGMP + diphosphate	-	?
dGTP + H2O	-	Homo sapiens	dGMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
MTH1	-	Homo sapiens
NUDT1	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0015	-	pH and temperature not specified in the publication	Homo sapiens	6-(methylamino)pyrimidine-2,4(1H,3H)-dione
0.0045	-	pH and temperature not specified in the publication	Homo sapiens	3-[(2-amino-6-methylpyrimidin-4-yl)oxy]propan-1-ol
0.059	-	pH and temperature not specified in the publication	Homo sapiens	9-methyl-2-(methylamino)-1,9-dihydro-6H-purin-6-one
0.08	-	IC50 above 0.01 mM, pH and temperature not specified in the publication	Homo sapiens	N-(1-methyl-1H-benzimidazol-5-yl)acetamide
0.1	-	IC50 above 0.1 mM, pH and temperature not specified in the publication	Homo sapiens	2-(dimethylamino)-9-methyl-1,9-dihydro-6H-purin-6-one
0.1	-	IC50 above 0.1 mM, pH and temperature not specified in the publication	Homo sapiens	2-amino-9-methyl-1,9-dihydro-6H-purin-6-one
0.1	-	IC50 above 0.1 mM, pH and temperature not specified in the publication	Homo sapiens	2-amino-9-methyl-7,9-dihydro-1H-purine-6,8-dione

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Release 2023.1 (January 2023)