Crystallization (Comment) | Organism |
---|---|
substrate-docking studies point to a role of His-97 in catalysis by orientation, and to a bidentate water bridging the dinuclear metal center as the potential nucleophile | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
H97A | severe reduction of activtiy | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
ADP-ribose | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.071 | - |
ADP-ribose | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.19 | - |
cyclic ADP-ribose | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.76 | - |
2',3'-cAMP | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
1.7 | - |
2',3'-cAMP | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
2 | - |
CDP-glycerol | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
2.65 | - |
ADP | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
3.9 | - |
CDP-ethanolamine | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
6.3 | - |
CDP-glycerol | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
12 | - |
CDP-choline | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
19 | - |
ADP | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
31 | - |
CDP-ethanolamine | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
43 | - |
CDP-choline | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | about 25% of the activity with Mn2+ | Danio rerio | |
Mn2+ | required, similar activatory effects with all the substrates tested. Km values around 1 microM and 2.5 mM Mn2+ for ADP-ribose hydrolysis | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q7T291 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | - |
Danio rerio | ? | - |
? | |
ADP + H2O | - |
Danio rerio | AMP + phosphate | - |
? | |
ADP-ribose + H2O | - |
Danio rerio | AMP + D-ribose 5-phosphate | - |
? | |
CDP-choline + H2O | - |
Danio rerio | CMP + phosphocholine | - |
? | |
CDP-ethanolamine + H2O | - |
Danio rerio | CMP + phosphoethanolamine | - |
? | |
CDP-glycerol + H2O | - |
Danio rerio | CMP + phosphoglycerol | - |
? | |
cyclic ADP-ribose + H2O | - |
Danio rerio | N1-(5-phosphoribosyl)-AMP | - |
? | |
additional information | very poor substrate: cyclic ADP-ribose. No substrate: ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, AMP or 3',5'-cAMP | Danio rerio | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADPRibase-Mn | - |
Danio rerio |
Adprm | - |
Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
cyclic ADP-ribose | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.2 | - |
ADP | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.4 | - |
ADP-ribose | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.8 | - |
CDP-choline | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.8 | - |
CDP-glycerol | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
1.8 | - |
ADP | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
2.1 | - |
CDP-ethanolamine | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
16.9 | - |
2',3'-cAMP | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
18 | - |
ADP-ribose | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
29.7 | - |
2',3'-cAMP | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
67.2 | - |
CDP-choline | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
95.6 | - |
CDP-glycerol | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
125.2 | - |
CDP-ethanolamine | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Danio rerio |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
half-maximum activity | Danio rerio |
9.2 | - |
half-maximum activity | Danio rerio |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
ADP | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.02 | - |
CDP-choline | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.028 | - |
cyclic ADP-ribose | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.07 | - |
CDP-ethanolamine | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.12 | - |
CDP-glycerol | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
0.66 | - |
ADP | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
5.6 | - |
CDP-choline | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
5.9 | - |
ADP-ribose | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
18 | - |
2',3'-cAMP | mutant H97A, pH 7.5, temperature not specified in the publication | Danio rerio | |
22 | - |
2',3'-cAMP | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
32 | - |
CDP-ethanolamine | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
48 | - |
CDP-glycerol | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio | |
340 | - |
ADP-ribose | wild-type, pH 7.5, temperature not specified in the publication | Danio rerio |