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Literature summary for 3.6.1.5 extracted from
- Kinetic study of NTPDase immobilization and its effect of haemocompatibility on polyethylene terephthalate (2019), J. Biomat. Sci., 30, 437-449 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme in a two-step process: in the first step, carboxyl group on polyethylene terephthalate (PET) surface is activated by the crosslinker 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, and in the second step, the enzyme is covalently attached to the activated carboxyl group, method development and evaluation. Kinetic study of NTPDase immobilization and its effect of hemocompatibility on PET, overview. Surface morphology and chemical composition of the unmodified and modified PET films are examined using scanning electron microscope with energy dispersive X-ray spectroscopy analysis | Solanum tuberosum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic modeling of free and immobilized enzymes, overview | Solanum tuberosum | |
| 0.042 | - |
ADP | free enzyme, pH 7.4, 22°C | Solanum tuberosum |  |
| 0.056 | - |
ADP | immobilized enzyme, pH 7.4, 22°C | Solanum tuberosum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Solanum tuberosum | - |
AMP + phosphate | - |
? | |
| ATP + H2O | Solanum tuberosum | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Solanum tuberosum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Solanum tuberosum | AMP + phosphate | - |
? | |
| ATP + H2O | - |
Solanum tuberosum | ADP + phosphate | - |
? | |
| additional information | phosphate release is measured using the malachite green method | Solanum tuberosum | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTPDase | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Solanum tuberosum |
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Release 2023.1 (January 2023)
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