Literature summary for 3.6.1.5 extracted from

Beckenkamp, L.R.; Iser, I.C.; Onzi, G.R.; Fontoura, D.M.S.D.; Bertoni, A.P.S.; Sevigny, J.; Lenz, G.; Wink, M.R.
Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level (2019), Biochem. J., 476, 1637-1651 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene Entpdase1, the sequence is constructed comprising the rat coding sequence from Thr38 to Thr476, which removes the nucleotides coding for the hydrophobic regions at the N- and C-terminals, and fused to a sequence coding for the rat IL-2-derived leader sequence that allows the enzyme to be secreted. Donor constructs are driven by EF1A promoter. Stable recombinant expression of wild-type and mutant enzymes in C6 glioma cells by the non-viral transfection with transposon PiggyBac-based method, real-time PCR expression analysis, transfected cells secrete an enzymatically active wild-type soluble NTPDase1/CD39	Rattus norvegicus

Cloned (Comment)

Organism

gene Entpdase1, the sequence is constructed comprising the rat coding sequence from Thr38 to Thr476, which removes the nucleotides coding for the hydrophobic regions at the N- and C-terminals, and fused to a sequence coding for the rat IL-2-derived leader sequence that allows the enzyme to be secreted. Donor constructs are driven by EF1A promoter. Stable recombinant expression of wild-type and mutant enzymes in C6 glioma cells by the non-viral transfection with transposon PiggyBac-based method, real-time PCR expression analysis, transfected cells secrete an enzymatically active wild-type soluble NTPDase1/CD39

Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
E174A	site-directed mutagenesis, inactive mutant	Rattus norvegicus
additional information	efficient PiggyBac-mediated transposition of the recombinant soluble enzymes, method overview. Transfected cells secrete an enzymatically active wild-type soluble NTPDase1/CD39	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + H2O	Rattus norvegicus	-	AMP + phosphate	-	?
ATP + H2O	Rattus norvegicus	-	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P97687	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ADP + H2O	-	Rattus norvegicus	AMP + phosphate	-	?
ATP + H2O	-	Rattus norvegicus	ADP + phosphate	-	?
additional information	production of inorganic phosphate is measured using the malachite green method	Rattus norvegicus	?	-	-

Synonyms

Synonyms	Comment	Organism
ENTPD1	-	Rattus norvegicus
SolCD39/NTPDase1	-	Rattus norvegicus
soluble CD39	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Rattus norvegicus