BRENDA - Enzyme Database
show all sequences of 3.6.1.41

Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus

Sasaki, M.; Takegawa, K.; Kimura, Y.; FEBS Lett. 588, 3395-3402 (2014)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
5 mM, 13.2% residual activity; 5 mM, 2.8% residual activity
Myxococcus xanthus
glycerol phosphate
20 mM, 33.4% residual activity; 20 mM, 46.1% residual activity
Myxococcus xanthus
iodoacetic acid
10 mM, 58.8% residual activity
Myxococcus xanthus
N-ethylmaleimide
1 mM, 62.6% residual activity; 1 mM, 73.8% residual activity
Myxococcus xanthus
sodium orthovanadate
1 mM, 6.7% residual activity
Myxococcus xanthus
trisodium phosphate
20 mM, 24.8% residual activity; 20 mM, 6.2% residual activity
Myxococcus xanthus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.19
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.49
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
0.86
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
about 50% of the activity with Mn2+. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17; required for ApnA hydrolysis
Myxococcus xanthus
Mn2+
best activator. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17
Myxococcus xanthus
Organism
Organism
UniProt
Commentary
Textmining
Myxococcus xanthus
Q1CWE7
-
-
Myxococcus xanthus
Q1DC62
-
-
Myxococcus xanthus DK 1622
Q1CWE7
-
-
Myxococcus xanthus DK 1622
Q1DC62
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
733872
Myxococcus xanthus
?
-
-
-
-
additional information
PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
733872
Myxococcus xanthus
?
-
-
-
-
additional information
enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
733872
Myxococcus xanthus DK 1622
?
-
-
-
-
additional information
PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
733872
Myxococcus xanthus DK 1622
?
-
-
-
-
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus
2 ADP
-
-
-
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus
2 ADP
in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
-
-
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus DK 1622
2 ADP
-
-
-
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus DK 1622
2 ADP
in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
-
-
?
P1,P5-bis(adenosyl)pentaphosphate + H2O
-
733872
Myxococcus xanthus
ADP + ATP
-
-
-
?
P1,P5-bis(adenosyl)pentaphosphate + H2O
-
733872
Myxococcus xanthus DK 1622
ADP + ATP
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
APAH
-
Myxococcus xanthus
MXAN_1509
-
Myxococcus xanthus
MXAN_7163
-
Myxococcus xanthus
PrpA
-
Myxococcus xanthus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.035
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.055
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
7.12
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
24
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
5 mM, 13.2% residual activity
Myxococcus xanthus
EDTA
5 mM, 2.8% residual activity
Myxococcus xanthus
glycerol phosphate
20 mM, 46.1% residual activity
Myxococcus xanthus
glycerol phosphate
20 mM, 33.4% residual activity
Myxococcus xanthus
iodoacetic acid
10 mM, 58.8% residual activity
Myxococcus xanthus
N-ethylmaleimide
1 mM, 73.8% residual activity
Myxococcus xanthus
N-ethylmaleimide
1 mM, 62.6% residual activity
Myxococcus xanthus
sodium orthovanadate
1 mM, 6.7% residual activity
Myxococcus xanthus
trisodium phosphate
20 mM, 24.8% residual activity
Myxococcus xanthus
trisodium phosphate
20 mM, 6.2% residual activity
Myxococcus xanthus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.19
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.49
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
0.86
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
about 50% of the activity with Mn2+. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17
Myxococcus xanthus
Co2+
required for ApnA hydrolysis
Myxococcus xanthus
Mn2+
best activator. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17
Myxococcus xanthus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
733872
Myxococcus xanthus
?
-
-
-
-
additional information
PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
733872
Myxococcus xanthus
?
-
-
-
-
additional information
enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
733872
Myxococcus xanthus DK 1622
?
-
-
-
-
additional information
PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
733872
Myxococcus xanthus DK 1622
?
-
-
-
-
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus
2 ADP
-
-
-
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus
2 ADP
in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
-
-
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus DK 1622
2 ADP
-
-
-
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
-
733872
Myxococcus xanthus DK 1622
2 ADP
in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
-
-
?
P1,P5-bis(adenosyl)pentaphosphate + H2O
-
733872
Myxococcus xanthus
ADP + ATP
-
-
-
?
P1,P5-bis(adenosyl)pentaphosphate + H2O
-
733872
Myxococcus xanthus DK 1622
ADP + ATP
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.035
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.055
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
7.12
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
24
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.18
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.4
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
8.28
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
48.57
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.18
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
0.4
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
Myxococcus xanthus
8.28
-
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
48.57
-
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
Myxococcus xanthus
Other publictions for EC 3.6.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733872
Sasaki
Enzymatic characteristics of a ...
Myxococcus xanthus, Myxococcus xanthus DK 1622
FEBS Lett.
588
3395-3402
2014
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6
4
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2
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5
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11
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4
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4
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10
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4
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3
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11
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4
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4
4
697896
Guranowski
Novel diadenosine polyphosphat ...
Escherichia coli
FEBS J.
276
1546-1553
2009
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6
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1
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3
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1
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1
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2
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6
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1
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1
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1
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667066
Hu
Preparation, crystallization a ...
Shigella flexneri, Shigella flexneri 2a
Acta Crystallogr. Sect. F
61
1078-1080
2005
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-
1
1
-
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1
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6
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1
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2
1
1
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1
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1
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1
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1
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2
1
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-
654033
Guranowski
Selective splitting of 3'-aden ...
Escherichia coli
Acta Biochim. Pol.
50
123-130
2003
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4
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2
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1
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2
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654523
Guranowski
Adenosine-5'-O-phosphorylated ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochem. J.
373
635-640
2003
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-
1
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22
2
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45
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1
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3
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2
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22
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1
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22
22
2
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1
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3
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656153
Ismail
Regulation of dinucleoside pol ...
Salmonella enterica
J. Biol. Chem.
278
32602-32607
2003
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1
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1
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2
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1
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2
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1
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1
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1
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2
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209910
Guranowski
Regiospecificity of the hydrol ...
Escherichia coli
Biochemistry
33
235-240
1994
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2
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4
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246769
McLennan
Recognition of beta beta-subst ...
Escherichia coli
Biochemistry
28
3868-3875
1989
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2
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246768
Taylor
-
Phosphonate analogs of bis(5'- ...
Escherichia coli
Biochem. Soc. Trans.
16
713-714
1988
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1
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1
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246767
Guranowski
Phosphonate analogues of diade ...
Escherichia coli
Biochemistry
26
3425-3429
1987
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4
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3
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1
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4
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1
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246770
Plateau
Catabolism of bis(5-nucleosidy ...
Escherichia coli, Escherichia coli EM20031
Biochemistry
24
914-922
1985
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1
2
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3
2
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3
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1
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1
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18
1
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1
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1
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2
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1
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1
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18
1
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1
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246765
Guranowski
Catabolism of diadenosine 5,5- ...
Acidaminococcus fermentans, Escherichia coli, Pyrodictium occultum
J. Biol. Chem.
258
14784-14789
1983
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8
1
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6
1
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3
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1
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1
-
10
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
6
1
-
-
-
-
1
-
-
1
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246763
Barnes
Isolation and characterization ...
Physarum polycephalum
Biochemistry
21
6123-6128
1982
-
-
-
-
-
-
13
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246764
Garrison
Diadenosine 5,5-P1,P4-tetrapho ...
Physarum polycephalum
Biochemistry
21
6129-6133
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-