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Information on EC 3.6.1.41 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
for references in articles please use BRENDA:EC3.6.1.41
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EC Tree 3 Hydrolases
3.6 Acting on acid anhydrides
3.6.1 In phosphorus-containing anhydrides
3.6.1.41 bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
IUBMB Comments
Also acts on bis(5′-guanosyl) tetraphosphate and bis(5′-adenosyl) pentaphosphate and, more slowly, on some other polyphosphates, forming a nucleoside bisphosphate as one product in all cases [cf. EC 3.6.1.17 bis(5′-nucleosyl)-tetraphosphatase (asymmetrical)].
The enzyme appears in viruses and cellular organisms
- 3.6.1.41
-
lupin
- hydrolases
-
nudix
- amp
- 5',5'''-p1,p4-tetraphosphate
- luteus
- angustifolius
- phosphorothioate
-
apnas
showSynonyms
diadenosine tetraphosphate hydrolase, diadenosine polyphosphate hydrolase, (symmetrical) ap4a hydrolase, (symmetrical) dinucleoside tetraphosphatase, bis(5'-adenosyl) tetraphosphatase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(symmetrical) Ap4A hydrolase
adenosine tetraphosphate phosphodiesterase
-
-
-
-
Ap4A hydrolase
-
-
-
-
APAH
bis(5'-adenosyl) tetraphosphatase
-
-
-
-
diadenosine 5',5'''-P1,P4-tetraphosphatase
-
-
-
-
diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
-
-
-
-
diadenosine polyphosphate hydrolase
-
-
-
-
Diadenosine tetraphosphatase
-
-
-
-
diadenosine tetraphosphate hydrolase
diadenosinetetraphosphatase (symmetrical)
-
-
-
-
dinucleosidetetraphosphatase (symmetrical)
-
-
-
-
MXAN_1509
MXAN_7163
phosphatase, bis(5'-nucleosyl) tetra- (symmetrical)
-
-
-
-
PrpA
symmetrical diadenosine tetraphosphate hydrolase
-
-
-
-
diadenosine tetraphosphate hydrolase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of diphosphate bonds
hydrolysis of diphosphate bonds
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleosidebisphosphohydrolase
Also acts on bis(5'-guanosyl) tetraphosphate and bis(5'-adenosyl) pentaphosphate and, more slowly, on some other polyphosphates, forming a nucleoside bisphosphate as one product in all cases [cf. EC 3.6.1.17 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)].
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CAS REGISTRY NUMBER
COMMENTARY
85638-48-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-adenylated bis(5'-adenosyl) tetraphosphate + H2O
3'-adenylated ADP + ADP
-
Substrates: hydrolysis of 3'-adenylated Ap3A
Products: -
Products: -
?
3'-adenylated bis(5'-guanosyl) tetraphosphate + H2O
3'-adenylated GDP + GDP
-
Substrates: hydrolysis of 3'-adenylated Gp3G
Products: -
Products: -
?
adenosine 5'-P(1)-tetraphosphate-P(4)-5'''-cytidine + H2O
ADP + CDP
-
Substrates: -
Products: -
Products: -
?
adenosine 5'-P(1)-triphosphate-P(3)-5''-cytidine + H2O
ADP + CMP + CDP + AMP
-
Substrates: -
Products: -
Products: -
?
adenosine 5'-P(1)-triphosphate-P(3)-5''-guanosine + H2O
ADP + GMP
-
Substrates: -
Products: -
Products: -
?
ApCF2ppCF2pA + H2O
?
-
Substrates: at 0.2% the rate of P1,P4-bis(5'-adenosyl) tetraphosphate
Products: -
Products: -
?
Gp6G + H2O
GDP + guanosine 5'-tetraphosphate
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-(2'-deoxy)adenosyl)tetraphosphate + H2O
2 dADP
-
Substrates: -
Products: -
Products: -
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
ADP + ?
-
Substrates: hydrolysis of Ap6A
Products: -
Products: -
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
ADP + ATP
-
Substrates: -
Products: -
Products: -
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
ADP + ATP
-
Substrates: -
Products: -
Products: -
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
ADP + ATP
-
Substrates: -
Products: -
Products: -
?
diadenosine tetraphosphate + H2O
2 adenosine diphosphate
-
Substrates: -
Products: -
Products: -
?
diadenosine tetraphosphate + H2O
2 adenosine diphosphate
-
Substrates: -
Products: -
Products: -
?
Gp3G + H2O
guanosine 3'-diphosphate + guanosine 5'-phosphate
-
Substrates: -
Products: -
Products: -
?
Gp3G + H2O
guanosine 3'-diphosphate + guanosine 5'-phosphate
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
2 ADP
-
Substrates: hydrolysis of Ap4A
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
2 ADP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
-
Substrates: hydrolysis of Ap4A
Products: -
Products: -
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
GTP
-
Substrates: -
Products: -
Products: -
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
GTP
-
Substrates: -
Products: -
Products: -
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
GTP
-
Substrates: -
Products: -
Products: -
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
GTP
-
Substrates: -
Products: -
Products: -
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
GTP
-
Substrates: -
Products: -
Products: -
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
2 ADP
Substrates: -
Products: -
Products: -
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
2 ADP
Substrates: -
Products: in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
Products: in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
2 ADP
Substrates: -
Products: -
Products: -
?
P1,P4-bis(adenosyl)tetraphosphate + H2O
2 ADP
Substrates: -
Products: in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
Products: in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP
?
P1,P5-bis(adenosyl)pentaphosphate + H2O
ADP + ATP
Substrates: -
Products: -
Products: -
?
P1,P5-bis(adenosyl)pentaphosphate + H2O
ADP + ATP
Substrates: -
Products: -
Products: -
?
P1,P6-di(adenosine-5')hexaphosphate + H2O
ADP + adenosine 5'-tetraphosphate
-
Substrates: -
Products: -
Products: -
?
P1,P6-di(adenosine-5')hexaphosphate + H2O
ADP + adenosine 5'-tetraphosphate
-
Substrates: -
Products: -
Products: -
?
P1,P6-di(adenosine-5')hexaphosphate + H2O
ADP + adenosine 5'-tetraphosphate
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: hydrolase from Escherichia coli shows almost no cleavage of dinucleoside polyphosphate molecules modified in their ADP moieties
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
Products: -
Products: -
?
additional information
?
-
Substrates: PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
Products: -
Products: -
?
additional information
?
-
Substrates: PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
Products: -
Products: -
?
additional information
?
-
-
Substrates: PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP
Products: -
Products: -
?
additional information
?
-
Substrates: PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
Products: -
Products: -
?
additional information
?
-
Substrates: PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Mg2+
-
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations
Mn2+
Ca2+
-
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration
Co2+
about 50% of the activity with Mn2+. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17
Mn2+
best activator. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
A-5'-pCH2OpOCH2p-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pCH2OpOpOCH2p-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOCH2pCH2Op-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOCH2pOpCH2Op-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOpCH2OpOp-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOpOCH2pCH2OpOp-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
glycerol phosphate
20 mM, 33.4% residual activity; 20 mM, 46.1% residual activity
N-ethylmaleimide
1 mM, 62.6% residual activity; 1 mM, 73.8% residual activity
trisodium phosphate
20 mM, 24.8% residual activity; 20 mM, 6.2% residual activity
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
-
competitive with respect to Ap4A
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
-
-
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
-
-
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
-
competitive with respect to Ap4A
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
-
-
EDTA
5 mM, 13.2% residual activity; 5 mM, 2.8% residual activity
tetra(adenosine-5'-O-phosphorothio) pentaerythritol
-
-
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
-
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Malaria
Structural and functional attributes of malaria parasite diadenosine tetraphosphate hydrolase.
Neoplasms
Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins.
Neoplasms
Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding.
Neoplasms
Di-, tri- and tetra-5'-O-phosphorothioadenosyl substituted polyols as inhibitors of Fhit: Importance of the alpha-beta bridging oxygen and beta phosphorus replacement.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0026
P1,P4-bis(5'-adenosyl)tetraphosphate
-
and 0.037, biphasic kinetics
0.014
P1,P6-bis(5'-adenosyl)hexaphosphate
-
reaction medium contains 0.1 mM CoCl2
0.005
P1,P4-bis(5'-adenosyl) tetraphosphate
-
pH 7.6, 30°C
0.037
P1,P4-bis(5'-adenosyl) tetraphosphate
-
and 0.0026, biphasic kinetics
0.037
P1,P4-bis(5'-adenosyl) tetraphosphate
-
reaction medium contains 0.1 mM CoCl2
0.14
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
0.86
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
0.19
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
0.49
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.055
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
7.12
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
0.035
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
24
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.4
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
8.28
P1,P4-bis(adenosyl)tetraphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
0.18
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Mn2+
48.57
P1,P5-bis(adenosyl)pentaphosphate
pH 8.0, 50°C, presence of 1 mM Co2+
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0013
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
-
pH 7.6, 30°C
0.0029
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
-
pH 7.6, 30°C
0.0014
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
-
pH 7.6, 30°C
0.0039
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
-
pH 7.6, 30°C
0.00002
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
-
pH 7.6, 30°C
0.00004
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
-
pH 7.6, 30°C
0.0018
tetra(adenosine-5'-O-phosphorothio) pentaerythritol
-
pH 7.6, 30°C
0.00008
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
-
pH 7.6, 30°C
0.0017
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
-
pH 7.6, 30°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (18087 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
monomer