BRENDA - Enzyme Database show
show all sequences of 3.6.1.22

Identification of UshA as a major enzyme for NAD degradation in Escherichia coli

Wang, L.; Zhou, Y.J.; Ji, D.; Lin, X.; Liu, Y.; Zhang, Y.; Liu, W.; Zhao, Z.K.; Enzyme Microb. Technol. 58-59, 75-79 (2014)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0053
-
NAD+
pH 7.5, 30C
Escherichia coli
0.007
-
NADH
pH 7.5, 30C
Escherichia coli
0.0111
-
NMN
pH 7.5, 30C
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
B1XCS0
-
-
Escherichia coli DH10B
B1XCS0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + H2O
-
733748
Escherichia coli
AMP + NMN
-
-
-
?
NADH + H2O
-
733748
Escherichia coli
NMNH + AMP
-
-
-
?
NADH + H2O
-
733748
Escherichia coli DH10B
NMNH + AMP
-
-
-
?
NMN + H2O
-
733748
Escherichia coli
nicotinamide riboside + phosphate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10
-
NADH
pH 7.5, 30C
Escherichia coli
19.3
-
NAD+
pH 7.5, 30C
Escherichia coli
1492
-
NMN
pH 7.5, 30C
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0053
-
NAD+
pH 7.5, 30C
Escherichia coli
0.007
-
NADH
pH 7.5, 30C
Escherichia coli
0.0111
-
NMN
pH 7.5, 30C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + H2O
-
733748
Escherichia coli
AMP + NMN
-
-
-
?
NADH + H2O
-
733748
Escherichia coli
NMNH + AMP
-
-
-
?
NADH + H2O
-
733748
Escherichia coli DH10B
NMNH + AMP
-
-
-
?
NMN + H2O
-
733748
Escherichia coli
nicotinamide riboside + phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10
-
NADH
pH 7.5, 30C
Escherichia coli
19.3
-
NAD+
pH 7.5, 30C
Escherichia coli
1492
-
NMN
pH 7.5, 30C
Escherichia coli
General Information
General Information
Commentary
Organism
physiological function
deletion of the UshA gene leads to faster cell growth and improves extracellular NAD stability by 3fold under conditions similar to whole-cell biocatalysis
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
deletion of the UshA gene leads to faster cell growth and improves extracellular NAD stability by 3fold under conditions similar to whole-cell biocatalysis
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1400
-
NADH
pH 7.5, 30C
Escherichia coli
3700
-
NAD+
pH 7.5, 30C
Escherichia coli
135000
-
NMN
pH 7.5, 30C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1400
-
NADH
pH 7.5, 30C
Escherichia coli
3700
-
NAD+
pH 7.5, 30C
Escherichia coli
135000
-
NMN
pH 7.5, 30C
Escherichia coli
Other publictions for EC 3.6.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733748
Wang
Identification of UshA as a ma ...
Escherichia coli, Escherichia coli DH10B
Enzyme Microb. Technol.
58-59
75-79
2014
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1
1
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3
3
720498
Wang
Comparative analysis of mycoba ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis variant bovis
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1375-1391
2011
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2
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2
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10
-
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3
4
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8
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2
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8
2
1
1
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1
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2
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2
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10
-
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3
4
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2
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8
2
1
1
-
-
1
-
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2
2
-
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670632
Jambunathan
Analysis of Arabidopsis growth ...
Arabidopsis sp.
Planta
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2006
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4
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1
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1
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668217
Magni
Enzymology of NAD+ homeostasis ...
Homo sapiens
Cell. Mol. Life Sci.
61
19-34
2004
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1
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1
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1
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AbdelRaheim
The NADH diphosphatase encoded ...
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1
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1
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7
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1
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1
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1
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7
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655747
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1
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1
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1
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1
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1
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1
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Cloning and characterization o ...
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2000
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2
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4
4
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6
4
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6
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2
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-
-
-
-
19
3
-
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-
4
3
-
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2
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-
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4
-
4
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6
4
3
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2
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19
3
-
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-
4
3
-
-
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209958
Hagen
Detection and identification o ...
Rattus norvegicus
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1
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2
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1
1
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2
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1
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209957
Frick
Cloning, purification, and pro ...
Escherichia coli
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-
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1
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1
6
-
5
2
1
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2
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1
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13
1
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1
1
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1
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1
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1
1
6
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5
2
1
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1
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13
1
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1
1
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171771
Wagner
The pyridine-nucleotide cycle ...
Nicotiana tabacum
Planta
167
226-232
1986
-
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-
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1
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1
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4
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1
1
1
3
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1
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1
1
3
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171772
Wagner
Regulation in tobacco callus o ...
Nicotiana tabacum
Planta
168
408-413
1986
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209954
Falconer
-
Membrane association of NAD py ...
Salmonella enterica subsp. enterica serovar Typhimurium
Curr. Microbiol.
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1984
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1
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1
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171773
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Pyridine nucleotide cycle of S ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
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1981
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1
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2
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209952
Berghaeuser
-
A simple preparation of an enz ...
Solanum tuberosum
Biotechnol. Lett.
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339-344
1981
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1
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209951
Davies
An enzyme degrading reduced ni ...
Proteus vulgaris
Biochem. J.
175
669-674
1978
1
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5
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4
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Simple methods of preparing ni ...
Solanum tuberosum
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209949
Nakajima
Purification and properties of ...
Proteus vulgaris
Biochim. Biophys. Acta
293
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1973
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15
1
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3
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8
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1
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15
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8
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1
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209948
Anderson
Nicotinamide-adenin dinucleoti ...
Aedes aegypti
Biochem. J.
101
392-396
1966
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