Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | above 10 mM | Sulfolobus acidocaldarius | |
ATP | above 10 mM | Sulfolobus acidocaldarius | |
diphosphate | above 10 mM | Sulfolobus acidocaldarius | |
F- | 20 mM, 80% inhibition | Sulfolobus acidocaldarius | |
additional information | addition of EDTA up to 0.5 mM to the assay mixture does not result in any change in the PPase activity | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.161 | - |
diphosphate | pH 3.0, 55°C | Sulfolobus acidocaldarius | |
0.189 | - |
ATP | pH 3.0, 55°C | Sulfolobus acidocaldarius | |
0.237 | - |
ATP | pH 3.0, 55°C | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sulfolobus acidocaldarius | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | 20 mM, 155% stimulation | Sulfolobus acidocaldarius | |
MgCl2 | 20 mM, 163% stimulation | Sulfolobus acidocaldarius | |
additional information | divalent cations are not essential for the acid PPase activity | Sulfolobus acidocaldarius | |
NiCl2 | 20 mM, 156% stimulation | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
1 * 26000, SDS-PAGE | Sulfolobus acidocaldarius |
35000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | P50308 | - |
- |
Sulfolobus acidocaldarius 7 | P50308 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.8 | - |
pH 3.0, 55°C | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | hydrolyzed at 36.2% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
ADP + H2O | hydrolyzed at 36.2% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
ATP + H2O | hydrolyzed at 19.4% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
ATP + H2O | hydrolyzed at 19.4% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
cyclic tripolyphosphate + H2O | hydrolyzed at 32.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
cyclic tripolyphosphate + H2O | hydrolyzed at 32.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
diphosphate + H2O | as far as the release of phosphate, is concerned, diphosphate, is the best substrate | Sulfolobus acidocaldarius | 2 phosphate | - |
? | |
diphosphate + H2O | as far as the release of phosphate, is concerned, diphosphate, is the best substrate | Sulfolobus acidocaldarius 7 | 2 phosphate | - |
? | |
tripolyphosphate + H2O | hydrolyzed at 44.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
tripolyphosphate + H2O | hydrolyzed at 44.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
acid PPase | - |
Sulfolobus acidocaldarius |
D1C enzyme | - |
Sulfolobus acidocaldarius |
inorganic pyrophosphatase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Sulfolobus acidocaldarius |
75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the purified enzyme is less thermostable than the membrane-bound enzyme in situ. The remaining activity after incubation at 70°C, pH 7, for 15 min, is about 70 and 100% for the purified enzyme and the membrane enzyme, respectively | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
assay at | Sulfolobus acidocaldarius |
3 | 3.5 | - |
Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 4.5 | at pH 2.5 and 4.5, about 50% of the maximal activity is observed | Sulfolobus acidocaldarius |
Organism | Comment | Expression |
---|---|---|
Sulfolobus acidocaldarius | addition of phosphate and/or diphosphate, to the semi-synthetic media results in about twofold increase in the acid PPase activity. However, in the nutrient broth, no induction is observed even when diphosphate is supplemented | up |