Cloned (Comment) | Organism |
---|---|
gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity | Anabaena sp. |
gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity, expression in Escherichia coli strain XL1-blue | Synechocystis sp. |
gene ppa, DNA and amino acid sequence determination and analysis,phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity | Pseudanabaena sp. |
gene ppa, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity | Blastochloris viridis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
diphosphate | native enzyme | Synechocystis sp. | |
0.0029 | - |
diphosphate | - |
Pseudanabaena sp. | |
0.003 | - |
diphosphate | - |
Anabaena sp. | |
0.003 | - |
diphosphate | recombinant enzyme | Synechocystis sp. | |
0.0274 | - |
diphosphate | - |
Blastochloris viridis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Blastochloris viridis | - |
- |
soluble | - |
Synechocystis sp. | - |
- |
soluble | - |
Anabaena sp. | - |
- |
soluble | - |
Pseudanabaena sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 25% | Blastochloris viridis | |
Cu2+ | activates | Blastochloris viridis | |
Fe2+ | activates 34% | Blastochloris viridis | |
Mg2+ | activates, Mg2+ is the highly preferred cofactor for family I sPPases | Synechocystis sp. | |
Mg2+ | activates, Mg2+ is the highly preferred cofactor for family I sPPases | Anabaena sp. | |
Mg2+ | activates, Mg2+ is the highly preferred cofactor for family I sPPases | Pseudanabaena sp. | |
Mg2+ | activates, Mg2+ is the preferred cofactor for family I sPPases | Blastochloris viridis | |
Mn2+ | activates | Blastochloris viridis | |
additional information | the enzyme is absolutely dependent on divalent cations | Blastochloris viridis | |
additional information | the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, and Co2+ | Synechocystis sp. | |
additional information | the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, Zn2+, and Co2+ | Anabaena sp. | |
additional information | the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, Zn2+, and Co2+ | Pseudanabaena sp. | |
Zn2+ | 13% of the activity with Mg2+ | Synechocystis sp. | |
Zn2+ | activates 40% | Blastochloris viridis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | Pseudanabaena sp. |
20000 | - |
6 * 20000, SDS-PAGE | Anabaena sp. |
20000 | - |
12 * 20000 | Blastochloris viridis |
110000 | - |
gel filtration | Synechocystis sp. |
114000 | - |
native PAGE | Anabaena sp. |
120000 | - |
native PAGE | Synechocystis sp. |
240000 | - |
native PAGE | Blastochloris viridis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Blastochloris viridis | an essential and ubiquitous metal-dependent enzyme | 2 phosphate | - |
? | |
diphosphate + H2O | Synechocystis sp. | an essential and ubiquitous metal-dependent enzyme | 2 phosphate | - |
? | |
diphosphate + H2O | Anabaena sp. | an essential and ubiquitous metal-dependent enzyme | 2 phosphate | - |
? | |
diphosphate + H2O | Pseudanabaena sp. | an essential and ubiquitous metal-dependent enzyme | 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | P80562 | strain PCC 7120, gene ppa | - |
Blastochloris viridis | - |
gene ppa | - |
Pseudanabaena sp. | P58733 | strain PCC 6903, gene ppa | - |
Synechocystis sp. | P80507 | strain PCC 6803, gene ppa | - |
Purification (Comment) | Organism |
---|---|
native enzyme | Anabaena sp. |
native enzyme, and recombinant enzyme from Escherichia coli strain XL1-blue | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Blastochloris viridis | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Synechocystis sp. | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Anabaena sp. | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Pseudanabaena sp. | 2 phosphate | - |
? | |
diphosphate + H2O | an essential and ubiquitous metal-dependent enzyme | Blastochloris viridis | 2 phosphate | - |
? | |
diphosphate + H2O | an essential and ubiquitous metal-dependent enzyme | Synechocystis sp. | 2 phosphate | - |
? | |
diphosphate + H2O | an essential and ubiquitous metal-dependent enzyme | Anabaena sp. | 2 phosphate | - |
? | |
diphosphate + H2O | an essential and ubiquitous metal-dependent enzyme | Pseudanabaena sp. | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 20000 | Blastochloris viridis |
hexamer | 6 * 20000, SDS-PAGE | Anabaena sp. |
hexamer | 6 * 19 187, mass spectrometry | Synechocystis sp. |
oligomer | x * 20000, SDS-PAGE | Pseudanabaena sp. |
Synonyms | Comment | Organism |
---|---|---|
family I inorganic pyrophosphatase | - |
Blastochloris viridis |
family I inorganic pyrophosphatase | - |
Synechocystis sp. |
family I inorganic pyrophosphatase | - |
Anabaena sp. |
family I inorganic pyrophosphatase | - |
Pseudanabaena sp. |
soluble inorganic pyrophosphatase | - |
Blastochloris viridis |
soluble inorganic pyrophosphatase | - |
Synechocystis sp. |
soluble inorganic pyrophosphatase | - |
Anabaena sp. |
soluble inorganic pyrophosphatase | - |
Pseudanabaena sp. |
sPPAse | - |
Blastochloris viridis |
sPPAse | - |
Synechocystis sp. |
sPPAse | - |
Anabaena sp. |
sPPAse | - |
Pseudanabaena sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
800 | - |
diphosphate | recombinant enzyme | Synechocystis sp. | |
850 | - |
diphosphate | - |
Anabaena sp. | |
850 | - |
diphosphate | - |
Pseudanabaena sp. | |
916 | - |
diphosphate | native enzyme | Synechocystis sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Synechocystis sp. | column chromatofocusing | - |
4.7 |