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Literature summary for 3.5.4.9 extracted from

  • Sah, S.; Varshney, U.
    Impact of mutating the key residues of a bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Escherichia coli on its activities (2015), Biochemistry, 54, 3504-3513 .
    View publication on PubMed
Search for more literature for 3.5.4.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C58Y kcat/Km for 5,10-methenyltetrahydrofolate is 13.2fold lower than wild-type value Escherichia coli
D121A kcat/Km for 5,10-methenyltetrahydrofolate is 2348fold lower than wild-type value Escherichia coli
G122D kcat/Km for 5,10-methenyltetrahydrofolate is 283fold lower than wild-type value Escherichia coli
K54S very low activity with 5,10-methenyltetrahydrofolate Escherichia coli
Q98K very low activity with 5,10-methenyltetrahydrofolate Escherichia coli
R191E kcat/Km for 5,10-methenyltetrahydrofolate is 1.3fold higher than wild-type value Escherichia coli
Y50S very low activity with 5,10-methenyltetrahydrofolate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D Escherichia coli
0.015
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A Escherichia coli
0.026
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme Escherichia coli
0.028
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme R191E Escherichia coli
0.04
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O Escherichia coli
-
 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O Escherichia coli K12
-
 10-formyltetrahydrofolate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P24186
-
-
Escherichia coli K12 P24186
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli K12 10-formyltetrahydrofolate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A Escherichia coli
0.025
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D Escherichia coli
4.75
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
40.9
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme Escherichia coli
57.4
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme R191E Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.67
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A Escherichia coli
5.55
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D Escherichia coli
118.75
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
1573.1
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme Escherichia coli
2050
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme R191E Escherichia coli